PMID- 24893265
OWN - NLM
STAT- MEDLINE
DCOM- 20150629
LR  - 20211021
IS  - 1557-7716 (Electronic)
IS  - 1523-0864 (Print)
IS  - 1523-0864 (Linking)
VI  - 21
IP  - 14
DP  - 2014 Nov 10
TI  - The role of SUMO-1 in cardiac oxidative stress and hypertrophy.
PG  - 1986-2001
LID - 10.1089/ars.2014.5983 [doi]
AB  - AIMS: Small ubiquitin-like modifier type 1 (SUMO-1) has been shown to play a 
      critical role in the dysfunction of the cardiac isoform of sarcoplasmic reticulum 
      calcium ATPase (SERCA2a) pump in the setting of heart failure. In cardiac 
      hypertrophy, the role of SUMO-1 has not been defined and our study's goals were 
      to examine the effects of modulating SUMO-1 on the hypertrophic response both in 
      vitro and in vivo and to examine whether oxidative stress (during cardiac 
      hypertrophy) is abrogated by SUMO-1 gene transfer. RESULTS: In mice undergoing 
      transverse aortic constriction (TAC), SUMO-1 levels increased slightly during the 
      compensated stage of hypertrophy and then dropped sharply during the transition 
      to heart failure. In isolated cardiomyocytes, SUMO-1 gene transfer inhibited the 
      hypertrophic response in the presence of phenylephrine. Adeno-associated vector 
      type 9 (AAV9) gene transfer of SUMO-1 prevented the heart from undergoing 
      hypertrophy after TAC and prevented the development of left ventricular 
      dysfunction. Furthermore, SUMO-1 gene transfer blocked the negative effects of 
      H2O2 on SERCA2a activity in cardiac myocytes, while in vivo indices of oxidative 
      stress were decreased by SUMO-1 in cardiac hypertrophy and heart failure. 
      INNOVATION AND CONCLUSION: The results of this study indicate that 
      post-translational modifications of SERCA2a caused by the toxic environment of 
      the hypertrophied and failing myocardium can be prevented by SUMO-1. Antioxid. 
      Redox Signal. 21, 1986-2001.
FAU - Lee, Ahyoung
AU  - Lee A
AD  - 1 Department of Cardiology, Cardiovascular Research Center , Icahn School of 
      Medicine at Mount Sinai, New York, New York.
FAU - Jeong, Dongtak
AU  - Jeong D
FAU - Mitsuyama, Shinichi
AU  - Mitsuyama S
FAU - Oh, Jae Gyun
AU  - Oh JG
FAU - Liang, Lifan
AU  - Liang L
FAU - Ikeda, Yoshiyuki
AU  - Ikeda Y
FAU - Sadoshima, Junichi
AU  - Sadoshima J
FAU - Hajjar, Roger J
AU  - Hajjar RJ
FAU - Kho, Changwon
AU  - Kho C
LA  - eng
GR  - R01 HL091469/HL/NHLBI NIH HHS/United States
GR  - HHSN268201000045C/HL/NHLBI NIH HHS/United States
GR  - R01 HL117505/HL/NHLBI NIH HHS/United States
GR  - P20HL100396/HL/NHLBI NIH HHS/United States
GR  - R00 HL116645/HL/NHLBI NIH HHS/United States
GR  - R01 HL067724/HL/NHLBI NIH HHS/United States
GR  - HL117505 01A1/HL/NHLBI NIH HHS/United States
GR  - R01 HL112330/HL/NHLBI NIH HHS/United States
GR  - R01 AG023039/AG/NIA NIH HHS/United States
GR  - R01 HL102738/HL/NHLBI NIH HHS/United States
GR  - HL088434/HL/NHLBI NIH HHS/United States
GR  - R01 HL093183/HL/NHLBI NIH HHS/United States
GR  - K99 HL116645/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140804
PL  - United States
TA  - Antioxid Redox Signal
JT  - Antioxidants & redox signaling
JID - 100888899
RN  - 0 (SUMO-1 Protein)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Cardiomegaly/genetics/*metabolism/pathology
MH  - Cells, Cultured
MH  - Gene Targeting
MH  - Heart Failure/genetics/*metabolism/pathology
MH  - Mice
MH  - Myocardium/metabolism/pathology
MH  - Myocytes, Cardiac/metabolism/pathology
MH  - *Oxidative Stress
MH  - SUMO-1 Protein/*genetics/metabolism
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases/*metabolism
PMC - PMC4208582
EDAT- 2014/06/04 06:00
MHDA- 2015/06/30 06:00
PMCR- 2014/11/10
CRDT- 2014/06/04 06:00
PHST- 2014/06/04 06:00 [entrez]
PHST- 2014/06/04 06:00 [pubmed]
PHST- 2015/06/30 06:00 [medline]
PHST- 2014/11/10 00:00 [pmc-release]
AID - 10.1089/ars.2014.5983 [pii]
AID - 10.1089/ars.2014.5983 [doi]
PST - ppublish
SO  - Antioxid Redox Signal. 2014 Nov 10;21(14):1986-2001. doi: 10.1089/ars.2014.5983. 
      Epub 2014 Aug 4.