PMID- 24893272
OWN - NLM
STAT- MEDLINE
DCOM- 20150818
LR  - 20191210
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 86
IP  - 13
DP  - 2014 Jul 1
TI  - Water-soluble conjugated polymer as a platform for adenosine deaminase sensing 
      based on fluorescence resonance energy transfer technique.
PG  - 6433-8
LID - 10.1021/ac500837f [doi]
AB  - We report a new biosensor for adenosine deaminase (ADA) sensing based on 
      water-soluble conjugated poly(9,9-bis(6'-N,N,N-trimethylammonium)hexyl)fluorine 
      phenylene (PFP) and fluorescence resonance energy transfer technique. In this 
      biosensor, PFP, DNAc-FI labeled with fluorescein (FAM), and ethidium bromide (EB) 
      were used as the fluorescence energy donor, resonance gate, and the final 
      fluorescence energy acceptor, respectively. In the absence of ADA, the adenosine 
      aptamer forms a hairpin-like conformation with adenosine, which is far from its 
      complementary single-stranded DNA (DNAc-FI). When PFP is excited at 380 nm, 
      fluorescein emits strong green fluorescence via one-step FRET while EB has no 
      fluorescence. After addition of ADA, adenosine is hydrolyzed to inosine and then 
      double-stranded DNA (dsDNA) is formed between the aptamer and DNAc-FI, followed 
      by EB intercalating into dsDNA. Once PFP is excited, EB will emit strong yellow 
      fluorescence after two-step FRET from PFP to fluorescein and from fluorescein to 
      EB. The sensitive ADA detection then is realized with a low detection limit of 
      0.5 U/L by measuring the FRET ratio of EB to fluorescein. Most importantly, the 
      assay is accomplished homogeneously in 25 min without further treatments, which 
      is much more simple and rapid than that reported in literature. Hence, this 
      method demonstrates the sensitive, cost-effective, and rapid detection of ADA 
      activity. It also opens an opportunity for designing promising sensors for other 
      enzymes.
FAU - Wang, Chun
AU  - Wang C
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, 
      Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, 
      School of Chemistry and Chemical Engineering, Shaanxi Normal University , Xi'an 
      710062, People's Republic of China.
FAU - Tang, Yanli
AU  - Tang Y
FAU - Liu, Yue
AU  - Liu Y
FAU - Guo, Yang
AU  - Guo Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140612
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (poly(9,9-bis(6'-N,N,N-trimethylammonium)hexyl)fluorine phenylene)
RN  - 059QF0KO0R (Water)
RN  - EC 3.5.4.4 (Adenosine Deaminase)
RN  - EN464416SI (Ethidium)
RN  - K72T3FS567 (Adenosine)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Adenosine/chemistry
MH  - Adenosine Deaminase/*analysis
MH  - Animals
MH  - Aptamers, Nucleotide/chemistry
MH  - Biosensing Techniques/methods
MH  - Cattle
MH  - Enzyme Assays/methods
MH  - Ethidium/chemistry
MH  - Fluorescein/chemistry
MH  - Fluorescence Resonance Energy Transfer/*methods
MH  - Humans
MH  - Limit of Detection
MH  - Quaternary Ammonium Compounds/*chemistry
MH  - Water/chemistry
EDAT- 2014/06/04 06:00
MHDA- 2015/08/19 06:00
CRDT- 2014/06/04 06:00
PHST- 2014/06/04 06:00 [entrez]
PHST- 2014/06/04 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
AID - 10.1021/ac500837f [doi]
PST - ppublish
SO  - Anal Chem. 2014 Jul 1;86(13):6433-8. doi: 10.1021/ac500837f. Epub 2014 Jun 12.