PMID- 24895123
OWN - NLM
STAT- MEDLINE
DCOM- 20150211
LR  - 20221207
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 289
IP  - 30
DP  - 2014 Jul 25
TI  - An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via 
      sialylation.
PG  - 20694-705
AB  - Recently, the Golgi phosphoprotein 3 (GOLPH3) and its yeast homolog Vps74p have 
      been characterized as essential for the Golgi localization of glycosyltransferase 
      in yeast. GOLPH3 has been identified as a new oncogene that is commonly amplified 
      in human cancers to modulate mammalian target of rapamycin signaling. However, 
      the molecular mechanisms of the carcinogenic signaling pathway remain largely 
      unclear. To investigate whether the expression of GOLPH3 was involved in the 
      glycosylation processes in mammalian cells, and whether it affected cell 
      behavior, we performed a loss-of-function study. Cell migration was suppressed in 
      GOLPH3 knockdown (KD) cells, and the suppression was restored by a 
      re-introduction of the GOLPH3 gene. HPLC and LC/MS analysis showed that the 
      sialylation of N-glycans was specifically decreased in KD cells. The specific 
      interaction between sialyltransferases and GOLPH3 was important for the 
      sialylation. Furthermore, overexpression of alpha2,6-sialyltransferase-I rescued cell 
      migration and cellular signaling, both of which were blocked in GOLPH3 knockdown 
      cells. These results are the first direct demonstration of the role of GOLPH3 in 
      N-glycosylation to regulate cell biological functions.
FAU - Isaji, Tomoya
AU  - Isaji T
FAU - Im, Sanghun
AU  - Im S
FAU - Gu, Wei
AU  - Gu W
FAU - Wang, Yuqin
AU  - Wang Y
FAU - Hang, Qinglei
AU  - Hang Q
FAU - Lu, Jishun
AU  - Lu J
FAU - Fukuda, Tomohiko
AU  - Fukuda T
FAU - Hashii, Noritaka
AU  - Hashii N
FAU - Takakura, Daisuke
AU  - Takakura D
FAU - Kawasaki, Nana
AU  - Kawasaki N
FAU - Miyoshi, Hiroyuki
AU  - Miyoshi H
FAU - Gu, Jianguo
AU  - Gu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (GOLPH3 protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
RN  - EC 2.4.99.1 (beta-D-Galactoside alpha 2-6-Sialyltransferase)
SB  - IM
MH  - Cell Movement/*physiology
MH  - Gene Knockdown Techniques
MH  - Glycosylation
MH  - HeLa Cells
MH  - Humans
MH  - Membrane Proteins/genetics/*metabolism
MH  - N-Acetylneuraminic Acid/genetics/*metabolism
MH  - Phosphoproteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Sialyltransferases/genetics/metabolism
MH  - Signal Transduction/*physiology
MH  - beta-D-Galactoside alpha 2-6-Sialyltransferase
PMC - PMC4110280
EDAT- 2014/06/05 06:00
MHDA- 2015/02/12 06:00
PMCR- 2015/07/25
CRDT- 2014/06/05 06:00
PHST- 2014/06/05 06:00 [entrez]
PHST- 2014/06/05 06:00 [pubmed]
PHST- 2015/02/12 06:00 [medline]
PHST- 2015/07/25 00:00 [pmc-release]
AID - S0021-9258(20)47590-8 [pii]
AID - M113.542688 [pii]
AID - 10.1074/jbc.M113.542688 [doi]
PST - ppublish
SO  - J Biol Chem. 2014 Jul 25;289(30):20694-705. doi: 10.1074/jbc.M113.542688.