PMID- 24896329
OWN - NLM
STAT- MEDLINE
DCOM- 20150207
LR  - 20220224
IS  - 2768-6698 (Electronic)
IS  - 2768-6698 (Linking)
VI  - 19
IP  - 6
DP  - 2014 Jun 1
TI  - Hyperhomocysteinemia promotes vascular remodeling in vein graph in mice.
PG  - 958-66
AB  - This study investigated the role and mechanism of Hyperhomocysteinemia (HHcy) on 
      vascular remodeling in mice. We assessed the effect of HHcy on vascular 
      remodeling using a carotid arterial vein patch model in mice with the gene 
      deletion of cystathionine-beta-synthase (Cbs). Vein grafts were harvested 4 weeks 
      after surgery. Cross sections were analyzed using Verhoeff-van Gieson staining, 
      Masson;s Trichrome staining, and immunostaining for morphological analysis and 
      protein level assessment. The effect of Hcy on collagen secretion was examined in 
      cultured rat aortic smooth muscle cells (RASMC). We found that Cbs-/- mice with 
      severe HHcy exhibited thicker neointima and a higher percentage of luminal 
      narrowing in vein grafts. In addition, severe HHcy increased elastin and collagen 
      deposition in the neointima. Further, severe HHcy increases CD45 positive cells 
      and proliferative cells in vein grafts. Finally, Hcy increases collagen secretion 
      in RASMC. These results demonstrate that HHcy increases neointima formation, 
      elastin and collagen deposition following a carotid arterial vein patch. The 
      capacity of Hcy to promote vascular fibrosis and inflammation may contribute to 
      the development of vascular remodeling.
FAU - Tan, Hongmei
AU  - Tan H
AD  - Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen 
      University, #74 Zhonshan Road 2, Guangzhou, China 510080.
FAU - Shi, Chengwei
AU  - Shi C
AD  - Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen 
      University, #74 Zhonshan Road 2, Guangzhou, China 510080.
FAU - Jiang, Xiaohua
AU  - Jiang X
AD  - Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen 
      University, #74 Zhonshan Road 2, Guangzhou, China 510080.
FAU - Lavelle, Muriel
AU  - Lavelle M
AD  - Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen 
      University, #74 Zhonshan Road 2, Guangzhou, China 510080.
FAU - Yu, Caijia
AU  - Yu C
AD  - Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen 
      University, #74 Zhonshan Road 2, Guangzhou, China 510080.
FAU - Yang, Xiaofeng
AU  - Yang X
AD  - Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen 
      University, #74 Zhonshan Road 2, Guangzhou, China 510080.
FAU - Wang, Hong
AU  - Wang H
AD  - Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen 
      University, #74 Zhonshan Road 2, Guangzhou, China 510080.
LA  - eng
GR  - HL82774/HL/NHLBI NIH HHS/United States
GR  - R01 HL108910/HL/NHLBI NIH HHS/United States
GR  - R01 HL077288/HL/NHLBI NIH HHS/United States
GR  - HL108910/HL/NHLBI NIH HHS/United States
GR  - R01 HL117654/HL/NHLBI NIH HHS/United States
GR  - HL67033/HL/NHLBI NIH HHS/United States
GR  - R01 HL082774/HL/NHLBI NIH HHS/United States
GR  - R01 HL110764/HL/NHLBI NIH HHS/United States
GR  - HL110764/HL/NHLBI NIH HHS/United States
GR  - HL116917/HL/NHLBI NIH HHS/United States
GR  - R01 HL094451/HL/NHLBI NIH HHS/United States
GR  - HL9445/HL/NHLBI NIH HHS/United States
GR  - F32 HL009445/HL/NHLBI NIH HHS/United States
GR  - HL77288/HL/NHLBI NIH HHS/United States
GR  - HL117654/HL/NHLBI NIH HHS/United States
GR  - R01 HL067033/HL/NHLBI NIH HHS/United States
GR  - R01 HL116917/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140601
PL  - Singapore
TA  - Front Biosci (Landmark Ed)
JT  - Frontiers in bioscience (Landmark edition)
JID - 101612996
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 9007-34-5 (Collagen)
RN  - 9007-58-3 (Elastin)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 4.2.1.22 (Cystathionine beta-Synthase)
RN  - Homocysteinemia
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Carotid Arteries/metabolism/*surgery
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Collagen/metabolism
MH  - Cystathionine beta-Synthase/genetics/metabolism
MH  - Elastin/metabolism
MH  - Homocysteine/pharmacology
MH  - Hyperhomocysteinemia/genetics/*metabolism
MH  - Jugular Veins/metabolism/*transplantation
MH  - Leukocyte Common Antigens/metabolism
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Muscle, Smooth, Vascular/cytology
MH  - Myocytes, Smooth Muscle/drug effects/metabolism
MH  - Neointima/genetics/metabolism
MH  - Rats
MH  - *Vascular Remodeling
PMC - PMC4331018
MID - NIHMS662419
EDAT- 2014/06/05 06:00
MHDA- 2015/02/11 06:00
PMCR- 2015/02/17
CRDT- 2014/06/05 06:00
PHST- 2014/06/05 06:00 [entrez]
PHST- 2014/06/05 06:00 [pubmed]
PHST- 2015/02/11 06:00 [medline]
PHST- 2015/02/17 00:00 [pmc-release]
AID - 4260 [pii]
AID - 10.2741/4260 [doi]
PST - epublish
SO  - Front Biosci (Landmark Ed). 2014 Jun 1;19(6):958-66. doi: 10.2741/4260.