PMID- 24898875
OWN - NLM
STAT- MEDLINE
DCOM- 20150622
LR  - 20141028
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 111
IP  - 12
DP  - 2014 Dec
TI  - Electrospun meshes possessing region-wise differences in fiber orientation, 
      diameter, chemistry and mechanical properties for engineering bone-ligament-bone 
      tissues.
PG  - 2549-59
LID - 10.1002/bit.25299 [doi]
AB  - Although bone-patellar tendon-bone (B-PT-B) autografts are the gold standard for 
      repair of anterior cruciate ligament ruptures, they suffer from drawbacks such as 
      donor site morbidity and limited supply. Engineered tissues modeled after B-PT-B 
      autografts are promising alternatives because they have the potential to 
      regenerate connective tissue and facilitate osseointegration. Towards the 
      long-term goal of regenerating ligaments and their bony insertions, the objective 
      of this study was to construct 2D meshes and 3D cylindrical composite scaffolds - 
      possessing simultaneous region-wise differences in fiber orientation, diameter, 
      chemistry and mechanical properties - by electrospinning two different polymers 
      from off-set spinnerets. Using a dual drum collector, 2D meshes consisting of an 
      aligned polycaprolactone (PCL) fiber region, randomly oriented 
      poly(lactide-co-glycolide) (PLGA) fiber region and a transition region (comprised 
      of both PCL and PLGA fibers) were prepared, and region-wise differences were 
      confirmed by microscopy and tensile testing. Bone marrow stromal cells (BMSCs) 
      cultured on these meshes exhibited random orientations and low aspect ratios on 
      the random PLGA regions, and high aspect ratios and alignment on the aligned PCL 
      regions. Next, meshes containing an aligned PCL region flanked by two transition 
      regions and two randomly oriented PLGA regions were prepared and processed into 
      3D cylindrical composite scaffolds using an interpenetrating photo-crosslinkable 
      polyethylene glycol diacrylate hydrogel to recapitulate the shape of B-PT-B 
      autografts. Tensile testing indicated that cylindrical composites were 
      mechanically robust, and eventually failed due to stress concentration in the 
      aligned PCL region. In summary, this study demonstrates a process to fabricate 
      electrospun meshes possessing region-wise differences in properties that can 
      elicit region-dependent cell responses, and be readily processed into scaffolds 
      with the shape of B-PT-B autografts.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Samavedi, Satyavrata
AU  - Samavedi S
AD  - Department of Chemical Engineering, Virginia Tech, Blacksburg, VA, 24061.
FAU - Vaidya, Prasad
AU  - Vaidya P
FAU - Gaddam, Prudhvidhar
AU  - Gaddam P
FAU - Whittington, Abby R
AU  - Whittington AR
FAU - Goldstein, Aaron S
AU  - Goldstein AS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140926
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Polyesters)
RN  - 102068-15-5 (peptide-Gly-Leu-amide)
RN  - 24980-41-4 (polycaprolactone)
SB  - IM
MH  - Animals
MH  - Antimicrobial Cationic Peptides/chemistry
MH  - Bone Marrow Cells
MH  - Bone and Bones/*cytology
MH  - Cells, Cultured
MH  - Electrochemical Techniques/*methods
MH  - Equipment Design
MH  - Female
MH  - Ligaments/*cytology
MH  - Polyesters/chemistry
MH  - Rats
MH  - Tensile Strength
MH  - Tissue Engineering/*methods
MH  - *Tissue Scaffolds
OTO - NOTNLM
OT  - bone marrow stromal cells
OT  - bone-ligament interface
OT  - contact guidance
OT  - electrospinning
OT  - fiber orientation
OT  - ligament tissue engineering
EDAT- 2014/06/06 06:00
MHDA- 2015/06/24 06:00
CRDT- 2014/06/06 06:00
PHST- 2014/03/12 00:00 [received]
PHST- 2014/05/28 00:00 [revised]
PHST- 2014/05/30 00:00 [accepted]
PHST- 2014/06/06 06:00 [entrez]
PHST- 2014/06/06 06:00 [pubmed]
PHST- 2015/06/24 06:00 [medline]
AID - 10.1002/bit.25299 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2014 Dec;111(12):2549-59. doi: 10.1002/bit.25299. Epub 2014 
      Sep 26.