PMID- 24901321
OWN - NLM
STAT- MEDLINE
DCOM- 20151005
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 6
DP  - 2014
TI  - Interactome profile of the host cellular proteins and the nonstructural protein 2 
      of porcine reproductive and respiratory syndrome virus.
PG  - e99176
LID - 10.1371/journal.pone.0099176 [doi]
LID - e99176
AB  - The nonstructural protein 2 (NSP2) is considered to be one of crucial viral 
      proteins in the replication and pathogenesis of porcine reproductive and 
      respiratory syndrome virus (PRRSV). In the present study, the host cellular 
      proteins that interact with the NSP2 of PRRSV were immunoprecipitated with 
      anti-Myc antibody from the MARC-145 cells infected by a recombinant PRRSV with 
      3xMyc tag insertion in its NSP2-coding region, and then 285 cellular proteins 
      interacting with NSP2 were identified by LC-MS/MS. The Gene Ontology and enriched 
      KEGG Pathway bioinformatics analyses indicated that the identified proteins could 
      be assigned to different subcellular locations and functional classes. Functional 
      analysis of the interactome profile highlighted cellular pathways associated with 
      infectious disease, translation, immune system, nervous system and signal 
      transduction. Two interested cellular proteins-BCL2-associated athanogene 6 
      (BAG6) and apoptosis-inducing factor 1 (AIF1) which may involve in transporting 
      of NSP2 to Endoplasmic reticulum (ER) or PRRSV-driven apoptosis were validated by 
      Western blot. The interactome data between PRRSV NSP2 and cellular proteins 
      contribute to the understanding of the roles of NSP2 in the replication and 
      pathogenesis of PRRSV, and also provide novel cellular target proteins for 
      elucidating the associated molecular mechanisms of the interaction of host 
      cellular proteins with viral proteins in regulating the viral replication.
FAU - Wang, Li
AU  - Wang L
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing, People's Republic of China.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing, People's Republic of China.
FAU - Zhang, Han
AU  - Zhang H
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing, People's Republic of China.
FAU - Li, Yan
AU  - Li Y
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing, People's Republic of China.
FAU - Ge, Xinna
AU  - Ge X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing, People's Republic of China.
FAU - Guo, Xin
AU  - Guo X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing, People's Republic of China.
FAU - Yu, Kangzhen
AU  - Yu K
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing, People's Republic of China; The Ministry 
      of Agriculture of the People's Republic of China, Beijing, People's Republic of 
      China.
FAU - Yang, Hanchun
AU  - Yang H
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140605
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Apoptosis Inducing Factor)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Apoptosis Inducing Factor/chemistry/*metabolism
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cricetinae
MH  - Endoplasmic Reticulum/metabolism
MH  - HEK293 Cells
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Immunoprecipitation
MH  - Molecular Chaperones/chemistry/*metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism
MH  - *Protein Interaction Maps
MH  - Proto-Oncogene Proteins c-myc/genetics/immunology/metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/chemistry/genetics
MH  - Swine
MH  - Transfection
MH  - Viral Nonstructural Proteins/chemistry/genetics/*metabolism
PMC - PMC4047090
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/06/06 06:00
MHDA- 2015/10/06 06:00
PMCR- 2014/06/05
CRDT- 2014/06/06 06:00
PHST- 2014/01/17 00:00 [received]
PHST- 2014/05/12 00:00 [accepted]
PHST- 2014/06/06 06:00 [entrez]
PHST- 2014/06/06 06:00 [pubmed]
PHST- 2015/10/06 06:00 [medline]
PHST- 2014/06/05 00:00 [pmc-release]
AID - PONE-D-14-02284 [pii]
AID - 10.1371/journal.pone.0099176 [doi]
PST - epublish
SO  - PLoS One. 2014 Jun 5;9(6):e99176. doi: 10.1371/journal.pone.0099176. eCollection 
      2014.