PMID- 24901676
OWN - NLM
STAT- MEDLINE
DCOM- 20150202
LR  - 20191210
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 62
IP  - 27
DP  - 2014 Jul 9
TI  - Mast-cell-based fluorescence biosensor for rapid detection of major fish allergen 
      parvalbumin.
PG  - 6473-80
LID - 10.1021/jf501382t [doi]
AB  - In this study, we developed a rat basophilic leukemia cell (RBL-2H3) fluorescence 
      sensor to detect and identify the major fish allergen parvalbumin (PV). We 
      constructed and transfected a CD63-enhanced green fluorescent protein (EGFP) 
      plasmid into RBL cells through a highly efficient, lipid-mediated, 
      DNA-transfection procedure. Stable transfectant RBL cells were then obtained for 
      a cell fluorescence assay with confocal laser scanning microscopy. Results show 
      that the cell surface expression of CD63 reflects degranulation, indicating that 
      a fluorescence assay with these cells could efficiently measure the activation of 
      antigen-stimulated transfectant cells and detect antigens with a nanogram level. 
      Therefore, this cell-based fluorescence biosensor technique for detecting fish PV 
      exhibits promise for quantifying fish PV after anti-PV immunoglobulin E (IgE) 
      stimulation. Results show that fluorescence intensities increased with purified 
      PV concentrations from 1 to 100 ng/mL, with a detection limit of 0.35 ng/mL 
      [relative standard deviation (RSD) of 4.5%], confirmed by beta-hexosaminidase 
      assays. These rat basophilic leukemia (RBL) mast cells transfected with the 
      CD63-EGFP gene and responded to PV only when they were sensitized with the 
      specific IgE antibody. This demonstrates the utility of this highly sensitive 
      biosensor for food allergen detection and prediction.
FAU - Jiang, Donglei
AU  - Jiang D
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan 
      University , Wuxi, Jiangsu 214122, People's Republic of China.
FAU - Jiang, Hui
AU  - Jiang H
FAU - Ji, Jian
AU  - Ji J
FAU - Sun, Xiulan
AU  - Sun X
FAU - Qian, He
AU  - Qian H
FAU - Zhang, Genyi
AU  - Zhang G
FAU - Tang, Lili
AU  - Tang L
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140626
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 0 (Parvalbumins)
RN  - 0 (Tetraspanin 30)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Allergens/*analysis/immunology
MH  - Animals
MH  - Biosensing Techniques/*methods
MH  - Cell Line
MH  - Fishes
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Mast Cells/*chemistry/immunology
MH  - Mice
MH  - Parvalbumins/*analysis/immunology
MH  - Plasmids/genetics
MH  - Rats
MH  - Tetraspanin 30/genetics/immunology
MH  - Transfection
EDAT- 2014/06/06 06:00
MHDA- 2015/02/03 06:00
CRDT- 2014/06/06 06:00
PHST- 2014/06/06 06:00 [entrez]
PHST- 2014/06/06 06:00 [pubmed]
PHST- 2015/02/03 06:00 [medline]
AID - 10.1021/jf501382t [doi]
PST - ppublish
SO  - J Agric Food Chem. 2014 Jul 9;62(27):6473-80. doi: 10.1021/jf501382t. Epub 2014 
      Jun 26.