PMID- 24903066
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR  - 20140815
IS  - 2047-2927 (Electronic)
IS  - 2047-2919 (Linking)
VI  - 2
IP  - 5
DP  - 2014 Sep
TI  - Detection and quantification of human herpes viruses types 4-6 in sperm samples 
      of patients with fertility disorders and chronic inflammatory urogenital tract 
      diseases.
PG  - 687-94
LID - 10.1111/j.2047-2927.2014.00232.x [doi]
AB  - Acute and chronic infections of the seminal tract are among the most common 
      causes of male infertility. As at least half of male infertility cases are 
      classified as idiopathic, some of these cases might be attributed to asymptomatic 
      infection. The detection and quantification of Epstein-Barr virus (EBV), 
      cytomegalovirus (CMV) and human herpes virus type 6 (HHV-6) DNA in semen samples 
      were performed. A total of 232 patients were divided into five groups: (i) 
      infertile men with varicocoele; (ii) men with idiopathic infertility; (iii) 
      infertile men with chronic inflammatory urogenital tract diseases (IUTD); (iv) 
      fertile men with IUTD and (v) men whose partners had a history of pregnancy loss. 
      In the study population, the prevalence of viral DNA was 17.7, 3.4% for EBV, 5.2% 
      for CMV, 6.5% for HHV-6, 0.43% for EBV + CMV, 0.87% for EBV + HHV-6 and 1.3% for 
      CMV + HHV-6. The median viral loads for EBV, CMV and HHV-6 were 500, 2250 and 250 
      copies/mL respectively. Of the sperm cell fractions, derived from infected 
      samples 87.5% contained viral DNA. No association between EBV and fertility 
      disorders or IUTD was found. CMV detection was much higher in the group of 
      patients with infertility and concomitant IUTD compared with the other groups 
      combined (18.5% vs. 5.4%, p = 0.03) and associated with reduced sperm cell count 
      (39.5 x 10(6) /mL vs. 72.5 x 10(6) /mL, p = 0.036). Immunostaining of spermatozoa 
      from infected samples and in vitro-infected cells detected CMV in sperm heads, 
      tails and connecting pieces and revealed attachment to sperm membrane and 
      intracellular localization. HHV-6 was the more common in fertile men with chronic 
      IUTD than in the other groups combined (19% vs. 6.3%, p = 0.018) and had no 
      effect on sperm parameters. The results suggest that both CMV and HHV-6 may 
      contribute to the aetiology of IUTD and, moreover, CMV-associated IUTD can lead 
      to male sterility.
CI  - (c) 2014 American Society of Andrology and European Academy of Andrology.
FAU - Naumenko, V
AU  - Naumenko V
AD  - D.I. Ivanovsky Institute of Virology, Moscow, Russia.
FAU - Tyulenev, Y
AU  - Tyulenev Y
FAU - Kurilo, L
AU  - Kurilo L
FAU - Shileiko, L
AU  - Shileiko L
FAU - Sorokina, T
AU  - Sorokina T
FAU - Evdokimov, V
AU  - Evdokimov V
FAU - Yakovleva, V
AU  - Yakovleva V
FAU - Kovalyk, V
AU  - Kovalyk V
FAU - Malolina, E
AU  - Malolina E
FAU - Kulibin, A
AU  - Kulibin A
FAU - Gomberg, M
AU  - Gomberg M
FAU - Kushch, A
AU  - Kushch A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140605
PL  - England
TA  - Andrology
JT  - Andrology
JID - 101585129
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Cytomegalovirus/isolation & purification
MH  - DNA, Viral/*isolation & purification
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 4, Human/isolation & purification
MH  - Herpesvirus 6, Human/isolation & purification
MH  - Humans
MH  - Infertility, Male/*virology
MH  - Male
MH  - Male Urogenital Diseases/*virology
MH  - Semen Analysis
MH  - Spermatozoa/virology
MH  - Urogenital System/virology
MH  - Varicocele
OTO - NOTNLM
OT  - Epstein-Barr virus
OT  - cytomegalovirus
OT  - human herpes virus type 6
OT  - infertility
OT  - pelvic pain syndrome
OT  - prostatitis
OT  - spermatozoa
OT  - uretheritis
EDAT- 2014/06/07 06:00
MHDA- 2015/05/13 06:00
CRDT- 2014/06/07 06:00
PHST- 2013/10/10 00:00 [received]
PHST- 2014/05/04 00:00 [revised]
PHST- 2014/05/07 00:00 [accepted]
PHST- 2014/06/07 06:00 [entrez]
PHST- 2014/06/07 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
AID - 10.1111/j.2047-2927.2014.00232.x [doi]
PST - ppublish
SO  - Andrology. 2014 Sep;2(5):687-94. doi: 10.1111/j.2047-2927.2014.00232.x. Epub 2014 
      Jun 5.