PMID- 24905764
OWN - NLM
STAT- MEDLINE
DCOM- 20151214
LR  - 20150320
IS  - 2161-5063 (Electronic)
IS  - 2161-5063 (Linking)
VI  - 4
IP  - 3
DP  - 2015 Mar 20
TI  - Mapping of amino acid substitutions conferring herbicide resistance in wheat 
      glutathione transferase.
PG  - 221-7
LID - 10.1021/sb500242x [doi]
AB  - We have used design of experiments (DOE) and systematic variance to efficiently 
      explore glutathione transferase substrate specificities caused by amino acid 
      substitutions. Amino acid substitutions selected using phylogenetic analysis were 
      synthetically combined using a DOE design to create an information-rich set of 
      gene variants, termed infologs. We used machine learning to identify and quantify 
      protein sequence-function relationships against 14 different substrates. The 
      resulting models were quantitative and predictive, serving as a guide for 
      engineering of glutathione transferase activity toward a diverse set of 
      herbicides. Predictive quantitative models like those presented here have broad 
      applicability for bioengineering.
FAU - Govindarajan, Sridhar
AU  - Govindarajan S
AD  - daggerDNA2.0, Inc., 1140 O'Brien Drive, Suite A, Menlo Park, California 94025, United 
      States.
FAU - Mannervik, Bengt
AU  - Mannervik B
AD  - double daggerDepartment of Neurochemistry, Stockholm University, SE-10691 Stockholm, Sweden.
FAU - Silverman, Joshua A
AU  - Silverman JA
AD  -  section signCalysta, 1140 O'Brien Drive, Suite B, Menlo Park, California 94025, United 
      States.
FAU - Wright, Kathy
AU  - Wright K
AD  - daggerDNA2.0, Inc., 1140 O'Brien Drive, Suite A, Menlo Park, California 94025, United 
      States.
FAU - Regitsky, Drew
AU  - Regitsky D
AD  -  section signCalysta, 1140 O'Brien Drive, Suite B, Menlo Park, California 94025, United 
      States.
FAU - Hegazy, Usama
AU  - Hegazy U
AD  -  parallelNational Research Centre, Dokki, Cairo 12311, Egypt.
FAU - Purcell, Thomas J
AU  - Purcell TJ
AD  - daggerDNA2.0, Inc., 1140 O'Brien Drive, Suite A, Menlo Park, California 94025, United 
      States.
FAU - Welch, Mark
AU  - Welch M
AD  - daggerDNA2.0, Inc., 1140 O'Brien Drive, Suite A, Menlo Park, California 94025, United 
      States.
FAU - Minshull, Jeremy
AU  - Minshull J
AD  - daggerDNA2.0, Inc., 1140 O'Brien Drive, Suite A, Menlo Park, California 94025, United 
      States.
FAU - Gustafsson, Claes
AU  - Gustafsson C
AD  - daggerDNA2.0, Inc., 1140 O'Brien Drive, Suite A, Menlo Park, California 94025, United 
      States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140613
PL  - United States
TA  - ACS Synth Biol
JT  - ACS synthetic biology
JID - 101575075
RN  - 0 (Plant Proteins)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution/*genetics
MH  - Glutathione Transferase/*chemistry/genetics/metabolism
MH  - Herbicide Resistance/*genetics
MH  - Machine Learning
MH  - Molecular Sequence Data
MH  - Plant Proteins/*chemistry/genetics/metabolism
MH  - Research Design
MH  - Sequence Analysis, Protein
MH  - Synthetic Biology/*methods
MH  - Triticum/*genetics
OTO - NOTNLM
OT  - bioengineering
OT  - design of experiment
OT  - enzymes
OT  - herbicide resistance
OT  - machine learning
OT  - optimization
OT  - sequence space
OT  - synthetic biology
EDAT- 2014/06/07 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/06/07 06:00
PHST- 2014/06/07 06:00 [entrez]
PHST- 2014/06/07 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1021/sb500242x [doi]
PST - ppublish
SO  - ACS Synth Biol. 2015 Mar 20;4(3):221-7. doi: 10.1021/sb500242x. Epub 2014 Jun 13.