PMID- 24906204 OWN - NLM STAT- MEDLINE DCOM- 20151026 LR - 20140911 IS - 1097-4644 (Electronic) IS - 0730-2312 (Linking) VI - 115 IP - 11 DP - 2014 Nov TI - Bone morphogenetic protein-10 induces cardiomyocyte proliferation and improves cardiac function after myocardial infarction. PG - 1868-76 LID - 10.1002/jcb.24856 [doi] AB - Heart disease is among the leading causes of death worldwide, and the limited proliferation of mammalian cardiomyocytes prevents heart regeneration in response to injury. Bone morphogenetic protein-10 (BMP10) exerts multiple roles in various developmental events; however, the effect of BMP10 and the underlying mechanism involved in cardiac repair remains unclear. After stimulation with the recombinant BMP10, an obvious dose-dependent cardiomyocyte proliferation and reentry of differentiated mammalian cardiomyocytes into the cell cycle was observed. Furthermore, BMP10 stimulation strikingly enhanced Tbx20 expression. Further analysis demonstrated that T-box 20 (Tbx20) was involved in BMP10-induced proliferation of differentiated cardiomyocytes as preconditioning with Tbx20 siRNA significantly attenuated BMP10-induced DNA synthesis. In vivo, BMP10 induced rat cardiomyocyte DNA synthesis and cytokinesis. After myocardial infarction (MI), BMP10 stimulated cardiomyocyte cell-cycle reentry and mitosis, resulting in the decrease of infarct size and improvement of cardiac repair. Taken together, these data indicated that BMP10 stimulated cardiomyocyte proliferation and repaired cardiac function after heart injury. Consequently, BMP10 may be a potential target for innovative strategies against heart failure. CI - (c) 2014 Wiley Periodicals, Inc. FAU - Sun, Lijun AU - Sun L AD - Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. FAU - Yu, Jing AU - Yu J FAU - Qi, Shun AU - Qi S FAU - Hao, Yuewen AU - Hao Y FAU - Liu, Ying AU - Liu Y FAU - Li, Zhenwu AU - Li Z LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Cell Biochem JT - Journal of cellular biochemistry JID - 8205768 RN - 0 (Bone Morphogenetic Proteins) RN - 0 (Recombinant Proteins) RN - 0 (T-Box Domain Proteins) RN - EC 3.4.24.19 (Bmp1 protein, rat) RN - EC 3.4.24.19 (Bone Morphogenetic Protein 1) SB - IM MH - Animals MH - Bone Morphogenetic Protein 1/*metabolism MH - Bone Morphogenetic Proteins/*metabolism MH - Cell Cycle MH - Cell Differentiation MH - Cell Proliferation MH - Genetic Therapy MH - Heart/*physiopathology MH - Heart Function Tests MH - Male MH - Myocardial Infarction/metabolism/physiopathology/*therapy MH - Myocytes, Cardiac/*cytology MH - Rats MH - Rats, Inbred Lew MH - Recombinant Proteins/administration & dosage MH - T-Box Domain Proteins/genetics/*metabolism OTO - NOTNLM OT - BMP10 OT - CARDIAC REPAIR OT - CARDIOMYOCYTE PROLIFERATION OT - MYOCARDIAL INFARCTION EDAT- 2014/06/07 06:00 MHDA- 2015/10/27 06:00 CRDT- 2014/06/07 06:00 PHST- 2014/02/11 00:00 [received] PHST- 2014/05/30 00:00 [accepted] PHST- 2014/06/07 06:00 [entrez] PHST- 2014/06/07 06:00 [pubmed] PHST- 2015/10/27 06:00 [medline] AID - 10.1002/jcb.24856 [doi] PST - ppublish SO - J Cell Biochem. 2014 Nov;115(11):1868-76. doi: 10.1002/jcb.24856.