PMID- 24906794
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20211021
IS  - 1557-3117 (Electronic)
IS  - 1053-2498 (Print)
IS  - 1053-2498 (Linking)
VI  - 33
IP  - 9
DP  - 2014 Sep
TI  - Efficacy of extracorporeal photopheresis in clearance of antibodies to 
      donor-specific and lung-specific antigens in lung transplant recipients.
PG  - 950-6
LID - S1053-2498(14)01118-8 [pii]
LID - 10.1016/j.healun.2014.04.020 [doi]
AB  - BACKGROUND: Extracorporeal photopheresis (ECP) has been used to treat chronic 
      rejection after lung transplantation (LTx). We investigated the effect of ECP on 
      several immune parameters that have been associated with poor lung function, 
      including donor-specific antibodies (DSA) to human leukocyte antigen (HLA), 
      antibodies against the lung-associated self-antigens (SAg), Kalpha1-tubulin (Kalpha1T), 
      collagen I and V, and circulating levels of pro-inflammatory and 
      anti-inflammatory cytokines. METHODS: Sera were collected from post-LTx patients 
      diagnosed with bronchiolitis obliterans before and 6 months after initiation of 
      ECP. DSA and cytokine levels were measured by Luminex (Invitrogen, Carlsbad, CA). 
      Changes in lung function over the 6 months preceding and after the initiation of 
      ECP were measured by retrospective analysis of spirometry performed at routine 
      clinic visits. RESULTS: ECP was associated with a significant decline in DSA 
      levels as well as antibodies to lung-associated SAg. ECP also reduced circulating 
      levels of pro-inflammatory cytokines and increased levels of anti-inflammatory 
      cytokines. These immunologic changes were associated with a significant 63% 
      reduction in the rate of decline in forced expiratory volume in 1 second over a 
      1-year period. Though statistically insignificant, a higher rate of clearance of 
      antibodies to lung-associated SAg was strongly associated with better response to 
      ECP. CONCLUSIONS: ECP is associated with a reduction in the levels of circulating 
      DSA, antibodies to lung-associated SAg (Kalpha1T, collagen I, and collagen V), and 
      circulating levels of several pro-inflammatory cytokines. We propose that these 
      changes contribute to the beneficial effect of ECP in reducing the decline in 
      lung function.
CI  - Copyright (c) 2014 International Society for Heart and Lung Transplantation. 
      Published by Elsevier Inc. All rights reserved.
FAU - Baskaran, Gautam
AU  - Baskaran G
AD  - Department of Medicine, Washington University School of Medicine, St. Louis, 
      Missouri.
FAU - Tiriveedhi, Venkataswarup
AU  - Tiriveedhi V
AD  - Department of Surgery, Washington University School of Medicine, St. Louis, 
      Missouri.
FAU - Ramachandran, Sabarinathan
AU  - Ramachandran S
AD  - Department of Surgery, Washington University School of Medicine, St. Louis, 
      Missouri.
FAU - Aloush, Aviva
AU  - Aloush A
AD  - Department of Medicine, Washington University School of Medicine, St. Louis, 
      Missouri.
FAU - Grossman, Brenda
AU  - Grossman B
AD  - Department of Pathology & Immunology, Washington University School of Medicine, 
      St. Louis, Missouri.
FAU - Hachem, Ramsey
AU  - Hachem R
AD  - Department of Medicine, Washington University School of Medicine, St. Louis, 
      Missouri.
FAU - Mohanakumar, Thalachallour
AU  - Mohanakumar T
AD  - Department of Surgery, Washington University School of Medicine, St. Louis, 
      Missouri; Department of Pathology & Immunology, Washington University School of 
      Medicine, St. Louis, Missouri. Electronic address: kumart@wustl.edu.
LA  - eng
GR  - R01 HL056643/HL/NHLBI NIH HHS/United States
GR  - HL-056643/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140509
PL  - United States
TA  - J Heart Lung Transplant
JT  - The Journal of heart and lung transplantation : the official publication of the 
      International Society for Heart Transplantation
JID - 9102703
RN  - 0 (Antibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type V)
RN  - 0 (Cytokines)
RN  - 0 (HLA Antigens)
RN  - 0 (TUBA1A protein, human)
RN  - 0 (Tubulin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antibodies/*blood
MH  - Autoantigens/*immunology
MH  - Bronchiolitis Obliterans/immunology/physiopathology/*surgery
MH  - Collagen Type I/immunology
MH  - Collagen Type V/immunology
MH  - Cytokines/blood
MH  - Female
MH  - Forced Expiratory Volume/physiology
MH  - Graft Rejection/blood/prevention & control
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Lung/*immunology
MH  - *Lung Transplantation
MH  - Male
MH  - Middle Aged
MH  - Photopheresis/*methods
MH  - Retrospective Studies
MH  - Tissue Donors
MH  - Transplant Recipients
MH  - Treatment Outcome
MH  - Tubulin/immunology
PMC - PMC4130746
MID - NIHMS602825
OTO - NOTNLM
OT  - chronic rejection
OT  - cytokines
OT  - donor-specific antibodies
OT  - extracorporeal photopheresis
OT  - lung transplantation
EDAT- 2014/06/08 06:00
MHDA- 2015/05/15 06:00
PMCR- 2015/09/01
CRDT- 2014/06/08 06:00
PHST- 2014/01/07 00:00 [received]
PHST- 2014/04/30 00:00 [revised]
PHST- 2014/04/30 00:00 [accepted]
PHST- 2014/06/08 06:00 [entrez]
PHST- 2014/06/08 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - S1053-2498(14)01118-8 [pii]
AID - 10.1016/j.healun.2014.04.020 [doi]
PST - ppublish
SO  - J Heart Lung Transplant. 2014 Sep;33(9):950-6. doi: 10.1016/j.healun.2014.04.020. 
      Epub 2014 May 9.