PMID- 24907761
OWN - NLM
STAT- MEDLINE
DCOM- 20150127
LR  - 20231213
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 41
DP  - 2014 Aug 1
TI  - Enhanced cytotoxicity and apoptosis-induced anticancer effect of silibinin-loaded 
      nanoparticles in oral carcinoma (KB) cells.
PG  - 274-82
LID - S0928-4931(14)00248-3 [pii]
LID - 10.1016/j.msec.2014.04.056 [doi]
AB  - Silibinin (SIL) is a plant derived flavonoid isolated from the fruits and seeds 
      of the milk thistle (Silybum marianum). Silibinin possesses a wide variety of 
      biological applications including anticancer activities but poor aqueous 
      solubility and poor bioavailability limit its potential and efficacy at the tumor 
      sites. In the present study, silibinin was encapsulated in Eudragit(R) E (EE) 
      nanoparticles in the presence of stabilizing agent polyvinyl alcohol (PVA) and 
      its anticancer efficacy in oral carcinoma (KB) cells was studied. Silibinin 
      loaded nanoparticles (SILNPs) were prepared by nanoprecipitation technique and 
      characterized in terms of size distribution, morphology, surface charge, 
      encapsulation efficiency and in vitro drug release. MTT assay revealed higher 
      cytotoxic efficacy of SILNPs than free SIL in KB cells. Meanwhile, reactive 
      oxygen species (ROS) determination revealed the significantly higher 
      intracellular ROS levels in SILNPs treated cells compared to free SIL treated 
      cells. Therefore, the differential cytotoxicity between SILNPs and SIL may be 
      mediated by the discrepancy of intracellular ROS levels. Moreover, acridine 
      orange (AO) and ethidium bromide (EB) dual staining and reduced mitochondrial 
      membrane potential (MMP) confirmed the induction of apoptosis with nanoparticle 
      treatment. Further, the extent of DNA damage (evaluated by comet assay) was 
      significantly increased in SILNPs than free SIL in KB cells. Taken together, the 
      present study suggests that silibinin-loaded nanoparticles can be used as an 
      effective drug delivery system to produce a better chemopreventive response for 
      the treatment of cancer.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Gohulkumar, M
AU  - Gohulkumar M
AD  - Department of Physics, Annamalai University, Annamalainagar 608 002, Tamilnadu, 
      India.
FAU - Gurushankar, K
AU  - Gurushankar K
AD  - Department of Physics, Annamalai University, Annamalainagar 608 002, Tamilnadu, 
      India.
FAU - Rajendra Prasad, N
AU  - Rajendra Prasad N
AD  - Department of Biochemistry and Biotechnology, Annamalai University, 
      Annamalainagar, 608 002, Tamilnadu, India.
FAU - Krishnakumar, N
AU  - Krishnakumar N
AD  - Department of Physics, Annamalai University, Annamalainagar 608 002, Tamilnadu, 
      India. Electronic address: nskumarphyamu@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20140502
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Silymarin)
RN  - 4RKY41TBTF (Silybin)
RN  - 9002-89-5 (Polyvinyl Alcohol)
SB  - IM
MH  - Antineoplastic Agents/*chemistry/pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - DNA Damage/drug effects
MH  - Humans
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Silybum marianum/chemistry/metabolism
MH  - Nanoparticles/*chemistry
MH  - Polyvinyl Alcohol/chemistry
MH  - Reactive Oxygen Species/metabolism
MH  - Silybin
MH  - Silymarin/*chemistry/*pharmacology
OTO - NOTNLM
OT  - Apoptosis
OT  - Cytotoxicity
OT  - KB cells
OT  - Nanoparticles
OT  - Silibinin
EDAT- 2014/06/09 06:00
MHDA- 2015/01/28 06:00
CRDT- 2014/06/09 06:00
PHST- 2013/12/02 00:00 [received]
PHST- 2014/03/09 00:00 [revised]
PHST- 2014/04/23 00:00 [accepted]
PHST- 2014/06/09 06:00 [entrez]
PHST- 2014/06/09 06:00 [pubmed]
PHST- 2015/01/28 06:00 [medline]
AID - S0928-4931(14)00248-3 [pii]
AID - 10.1016/j.msec.2014.04.056 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2014 Aug 1;41:274-82. doi: 
      10.1016/j.msec.2014.04.056. Epub 2014 May 2.