PMID- 24910201
OWN - NLM
STAT- MEDLINE
DCOM- 20141217
LR  - 20211021
IS  - 1090-2430 (Electronic)
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 261
DP  - 2014 Nov
TI  - TrkB kinase activity is critical for recovery of respiratory function after 
      cervical spinal cord hemisection.
PG  - 190-5
LID - S0014-4886(14)00186-1 [pii]
LID - 10.1016/j.expneurol.2014.05.027 [doi]
AB  - Neuroplasticity following spinal cord injury contributes to spontaneous recovery 
      over time. Recent studies highlight the important role of brain-derived 
      neurotrophic factor (BDNF) signaling via the high-affinity tropomyosin-related 
      kinase (Trk) receptor subtype B (TrkB) in recovery of rhythmic diaphragm activity 
      following unilateral spinal hemisection at C2 (C2SH). We hypothesized that TrkB 
      kinase activity is necessary for spontaneous recovery of diaphragm activity 
      post-C2SH. A chemical-genetic approach employing adult male TrkB(F616A) mice 
      (n=49) was used to determine the impact of inhibiting TrkB kinase activity by the 
      phosphoprotein phosphatase 1 inhibitor derivative 1NMPP1 on recovery of 
      ipsilateral hemidiaphragm EMG activity. In mice, C2SH was localized primarily to 
      white matter tracts comprising the lateral funiculus. The extent of damaged 
      spinal cord (~27%) was similar regardless of the presence of functional recovery, 
      consistent with spontaneous recovery reflecting neuroplasticity primarily of 
      contralateral spared descending pathways to the phrenic motor pools. Ipsilateral 
      hemidiaphragm EMG activity was verified as absent in all mice at 3days post-C2SH. 
      By 2weeks after C2SH, ipsilateral hemidiaphragm EMG activity was present in 39% 
      of vehicle-treated mice compared to 7% of 1NMPP1-treated mice (P=0.03). These 
      data support the hypothesis that BDNF/TrkB signaling involving TrkB kinase 
      activity plays a critical role in spontaneous recovery of diaphragm activity 
      following cervical spinal cord injury.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Mantilla, Carlos B
AU  - Mantilla CB
AD  - Department of Physiology and Biomedical Engineering, Mayo Clinic College of 
      Medicine, Rochester, MN 55905, USA; Department of Anesthesiology, Mayo Clinic 
      College of Medicine, Rochester, MN 55905, USA. Electronic address: 
      mantilla.carlos@mayo.edu.
FAU - Greising, Sarah M
AU  - Greising SM
AD  - Department of Physiology and Biomedical Engineering, Mayo Clinic College of 
      Medicine, Rochester, MN 55905, USA.
FAU - Stowe, Jessica M
AU  - Stowe JM
AD  - Department of Physiology and Biomedical Engineering, Mayo Clinic College of 
      Medicine, Rochester, MN 55905, USA.
FAU - Zhan, Wen-Zhi
AU  - Zhan WZ
AD  - Department of Physiology and Biomedical Engineering, Mayo Clinic College of 
      Medicine, Rochester, MN 55905, USA.
FAU - Sieck, Gary C
AU  - Sieck GC
AD  - Department of Physiology and Biomedical Engineering, Mayo Clinic College of 
      Medicine, Rochester, MN 55905, USA; Department of Anesthesiology, Mayo Clinic 
      College of Medicine, Rochester, MN 55905, USA.
LA  - eng
GR  - R01 HL096750/HL/NHLBI NIH HHS/United States
GR  - T32 HL105355/HL/NHLBI NIH HHS/United States
GR  - HL096750/HL/NHLBI NIH HHS/United States
GR  - HL105355/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140605
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (1NMPP1 compound)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Pyrazoles)
RN  - 0 (Pyrimidines)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Cervical Cord
MH  - Diaphragm/physiopathology
MH  - Disease Models, Animal
MH  - Electromyography
MH  - Functional Laterality/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Mutation/genetics
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Pyrazoles/pharmacology
MH  - Pyrimidines/pharmacology
MH  - Receptor, trkB/genetics/*metabolism
MH  - Recovery of Function/drug effects/*physiology
MH  - Respiration Disorders/*etiology
MH  - Spinal Cord Injuries/*complications/pathology
MH  - Time Factors
PMC - PMC4194245
MID - NIHMS619521
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - Diaphragm muscle
OT  - EMG
OT  - Spinal cord injury
EDAT- 2014/06/10 06:00
MHDA- 2014/12/18 06:00
PMCR- 2015/11/01
CRDT- 2014/06/10 06:00
PHST- 2014/01/24 00:00 [received]
PHST- 2014/05/27 00:00 [revised]
PHST- 2014/05/30 00:00 [accepted]
PHST- 2014/06/10 06:00 [entrez]
PHST- 2014/06/10 06:00 [pubmed]
PHST- 2014/12/18 06:00 [medline]
PHST- 2015/11/01 00:00 [pmc-release]
AID - S0014-4886(14)00186-1 [pii]
AID - 10.1016/j.expneurol.2014.05.027 [doi]
PST - ppublish
SO  - Exp Neurol. 2014 Nov;261:190-5. doi: 10.1016/j.expneurol.2014.05.027. Epub 2014 
      Jun 5.