PMID- 24911002
OWN - NLM
STAT- MEDLINE
DCOM- 20150109
LR  - 20140930
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 230
IP  - 1
DP  - 2015 Jan
TI  - A functional transient receptor potential vanilloid 4 (TRPV4) channel is 
      expressed in human endothelial progenitor cells.
PG  - 95-104
LID - 10.1002/jcp.24686 [doi]
AB  - Endothelial progenitor cells (EPCs) are mobilized into circulation to replace 
      damaged endothelial cells and recapitulate the vascular network of injured 
      tissues. Intracellular Ca(2+) signals are key to EPC activation, but it is yet to 
      be elucidated whether they are endowed with the same blend of Ca(2+) -permeable 
      channels expressed by mature endothelial cells. For instance, endothelial colony 
      forming cells (ECFCs), the only EPC subset truly committed to acquire a mature 
      endothelial phenotype, lack canonical transient receptor potential channels 3, 5 
      and 6 (TRPC3, 5 and 6), which are widely distributed in vascular endothelium; on 
      the other hand, they express a functional store-operated Ca(2+) entry (SOCE). The 
      present study was undertaken to assess whether human circulating EPCs possess TRP 
      vanilloid channel 4 (TRPV4), which plays a master signalling role in mature 
      endothelium, by controlling both vascular remodelling and arterial pressure. We 
      found that EPCs express both TRPV4 mRNA and protein. Moreover, both GSK1016790A 
      (GSK) and phorbol myristate acetate and, two widely employed TRPV4 agonists, 
      induced intracellular Ca(2+) signals uniquely in presence of extracellular 
      Ca(2+). GSK- and PMA-induced Ca(2+) elevations were inhibited by RN-1734 and 
      ruthenium red, which selectively target TRPV4 in mature endothelium. However, 
      TRPV4 stimulation with GSK did not cause EPC proliferation, while the 
      pharmacological blockade of TRPV4 only modestly affected EPC growth in the 
      presence of a growth factor-enriched culture medium. Conversely, SOCE inhibition 
      with BTP-2, La(3+) and Gd(3+) dramatically decreased cell proliferation. These 
      data indicate that human circulating EPCs possess a functional TRPV4 protein 
      before their engraftment into nascent vessels.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Dragoni, Silvia
AU  - Dragoni S
AD  - Department of Biology and Biotechnology "Lazzaro Spallanzani", University of 
      Pavia, Pavia, Italy.
FAU - Guerra, Germano
AU  - Guerra G
FAU - Fiorio Pla, Alessandra
AU  - Fiorio Pla A
FAU - Bertoni, Giuseppe
AU  - Bertoni G
FAU - Rappa, Alessandra
AU  - Rappa A
FAU - Poletto, Valentina
AU  - Poletto V
FAU - Bottino, Cinzia
AU  - Bottino C
FAU - Aronica, Adele
AU  - Aronica A
FAU - Lodola, Francesco
AU  - Lodola F
FAU - Cinelli, Maria Pia
AU  - Cinelli MP
FAU - Laforenza, Umberto
AU  - Laforenza U
FAU - Rosti, Vittorio
AU  - Rosti V
FAU - Tanzi, Franco
AU  - Tanzi F
FAU - Munaron, Luca
AU  - Munaron L
FAU - Moccia, Francesco
AU  - Moccia F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 
      (4-methyl-4'-(3,5-bis(trifluoromethyl)-1H-pyrazol-1-yl)-1,2,3-thiadiazole-5-carboxanilide)
RN  - 0 (Anilides)
RN  - 0 (Cation Transport Proteins)
RN  - 0 
      (N-(1-((4-(2-(((2,4-dichlorophenyl)sulfonyl)amino)-3-hydroxypropanoyl)-1-piperazinyl)carbonyl)-3-methylbutyl)-1-benzothiophene-2-carboxamide)
RN  - 0 (RN 1734)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sulfonamides)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (TRPV4 protein, human)
RN  - 0 (Thiadiazoles)
RN  - 11103-72-3 (Ruthenium Red)
RN  - GMW67QNF9C (Leucine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Anilides/pharmacology
MH  - Calcium/metabolism
MH  - Cation Transport Proteins/biosynthesis
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Endothelial Cells/cytology/*metabolism
MH  - Endothelium, Vascular/*metabolism
MH  - Humans
MH  - Leucine/analogs & derivatives/pharmacology
MH  - Neovascularization, Physiologic/*physiology
MH  - RNA, Messenger/biosynthesis
MH  - Ruthenium Red/pharmacology
MH  - Stem Cells/cytology/*metabolism
MH  - Sulfonamides/pharmacology
MH  - TRPV Cation Channels/agonists/antagonists & inhibitors/*biosynthesis/genetics
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Thiadiazoles/pharmacology
MH  - Young Adult
EDAT- 2014/06/10 06:00
MHDA- 2015/01/13 06:00
CRDT- 2014/06/10 06:00
PHST- 2014/03/31 00:00 [received]
PHST- 2014/05/22 00:00 [accepted]
PHST- 2014/06/10 06:00 [entrez]
PHST- 2014/06/10 06:00 [pubmed]
PHST- 2015/01/13 06:00 [medline]
AID - 10.1002/jcp.24686 [doi]
PST - ppublish
SO  - J Cell Physiol. 2015 Jan;230(1):95-104. doi: 10.1002/jcp.24686.