PMID- 24912661
OWN - NLM
STAT- MEDLINE
DCOM- 20150803
LR  - 20220311
IS  - 1097-0223 (Electronic)
IS  - 0197-3851 (Linking)
VI  - 34
IP  - 11
DP  - 2014 Nov
TI  - A new marker set that identifies fetal cells in maternal circulation with high 
      specificity.
PG  - 1066-72
LID - 10.1002/pd.4429 [doi]
AB  - OBJECTIVE: Fetal cells from the maternal circulation (FCMBs) have the potential 
      to replace cells from amniotic fluid or chorionic villi in a diagnosis of common 
      chromosomal aneuploidies. Good markers for enrichment and identification are 
      lacking. METHOD: Blood samples from 78 normal pregnancies were used for testing 
      the marker-set CD105 and CD141 for fetal cell enrichment. Fetal cell candidates 
      were subsequently stained by a cocktail of cytokeratin antibodies, and the gender 
      of the fetal cells was explored by fluorescence in situ hybridization (FISH) of 
      the X and Y chromosomes. RESULTS: Fetal cell candidates could be detected in 91% 
      of the samples, and in 85% of the samples, it was possible to obtain X and Y 
      chromosomal FISH results for gender determination. The concordance between gender 
      determined by FISH on fetal cells in maternal blood and gender found at birth 
      reached 100% if three or more fetal cells with FISH signals could be found in a 
      sample. CONCLUSION: The marker set identifies fetal cells with specificity high 
      enough to make cell-based noninvasive prenatal diagnosis realistic.
CI  - (c) 2014 John Wiley & Sons, Ltd.
FAU - Hatt, Lotte
AU  - Hatt L
AD  - Department of Clinical Genetics, Vejle Hospital, University of Southern Denmark, 
      Vejle, Denmark; FCMB ApS, Vejle, Denmark.
FAU - Brinch, Marie
AU  - Brinch M
FAU - Singh, Ripudaman
AU  - Singh R
FAU - Moller, Kristine
AU  - Moller K
FAU - Lauridsen, Rune Hoff
AU  - Lauridsen RH
FAU - Schlutter, Jacob Morup
AU  - Schlutter JM
FAU - Uldbjerg, Niels
AU  - Uldbjerg N
FAU - Christensen, Britta
AU  - Christensen B
FAU - Kolvraa, Steen
AU  - Kolvraa S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140629
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (Biomarkers)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Biomarkers/*blood
MH  - Blood Cells/*cytology
MH  - Female
MH  - Fetus/*cytology
MH  - Hematologic Tests
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Keratins/analysis/blood
MH  - Male
MH  - Mothers
MH  - Pregnancy/*blood
MH  - Pregnancy Trimester, First/blood
MH  - Prenatal Diagnosis/*methods
MH  - Sensitivity and Specificity
MH  - Sex Determination Analysis/methods
EDAT- 2014/06/11 06:00
MHDA- 2015/08/04 06:00
CRDT- 2014/06/11 06:00
PHST- 2014/02/03 00:00 [received]
PHST- 2014/05/09 00:00 [revised]
PHST- 2014/06/06 00:00 [accepted]
PHST- 2014/06/11 06:00 [entrez]
PHST- 2014/06/11 06:00 [pubmed]
PHST- 2015/08/04 06:00 [medline]
AID - 10.1002/pd.4429 [doi]
PST - ppublish
SO  - Prenat Diagn. 2014 Nov;34(11):1066-72. doi: 10.1002/pd.4429. Epub 2014 Jun 29.