PMID- 24913063
OWN - NLM
STAT- MEDLINE
DCOM- 20150403
LR  - 20140804
IS  - 1096-7206 (Electronic)
IS  - 1096-7192 (Linking)
VI  - 112
IP  - 4
DP  - 2014 Aug
TI  - Methionine-induced hyperhomocysteinemia and bleomycin hydrolase deficiency alter 
      the expression of mouse kidney proteins involved in renal disease.
PG  - 339-46
LID - S1096-7192(14)00172-3 [pii]
LID - 10.1016/j.ymgme.2014.05.010 [doi]
AB  - SCOPE: Hyperhomocysteinemia (HHcy) induced by dietary or genetic factors is 
      linked to kidney disease. Bleomycin hydrolase (Blmh) metabolizes Hcy-thiolactone 
      to Hcy. We aimed to explain the role of dietary HHcy in kidney disease. METHODS 
      AND RESULTS: We examined kidney proteome in dietary HHcy and Blmh-knockout mouse 
      models using 2D IEF/SDS-PAGE gel electrophoresis and MALDI-TOF mass spectrometry. 
      We found that the kidney proteome was altered by dietary HHcy and the Blmh(-/-) 
      genotype. Proteins involved in metabolism of lipoprotein (ApoA1), amino acid and 
      protein (Acy1, Hspd1), carbohydrate (Pdhb, Fbp1-isoform 1, Eno1), and energy 
      metabolism (Ndufs8, Ldhd) were down-regulated. Proteins involved in carbohydrate 
      metabolism (Fbp1-isoform 2), oxidative stress response (Prdx2), and 
      detoxification (Glod4) were up-regulated. The Blmh(-/-) genotype down-regulated 
      Glod4 isoform 3 mRNA but did not affect isoform 1 mRNA expression in mouse 
      kidneys, suggesting post-transcriptional regulation of the Glod4 protein by the 
      Blmh(+/+) genotype. Responses of ApoA1, Acy1, Hspd1, Ndufs8, Fbp1, Eno1, and 
      Prdx2 to HHcy and/or Blmh deficiency mimic their responses to renal disease. 
      CONCLUSION: Our findings indicate that Blmh interacts with diverse cellular 
      processes--lipoprotein, amino acid and protein, carbohydrate, and energy 
      metabolisms, detoxification, antioxidant defenses--that are essential for normal 
      kidney homeostasis and that deregulation of these processes can account for the 
      involvement of HHcy in kidney disease.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Suszynska-Zajczyk, Joanna
AU  - Suszynska-Zajczyk J
AD  - Institute of Bioorganic Chemistry, Poznan, Poland.
FAU - Utyro, Olga
AU  - Utyro O
AD  - Institute of Bioorganic Chemistry, Poznan, Poland; Department of Biochemistry and 
      Biotechnology, University of Life Sciences, Poznan, Poland.
FAU - Jakubowski, Hieronim
AU  - Jakubowski H
AD  - Institute of Bioorganic Chemistry, Poznan, Poland; Department of Biochemistry and 
      Biotechnology, University of Life Sciences, Poznan, Poland; Department of 
      Microbiology & Molecular Genetics, Rutgers-New Jersey Medical School, 
      International Center for Public Health, Newark, NJ, USA. Electronic address: 
      jakubows@njms.rutgers.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140524
PL  - United States
TA  - Mol Genet Metab
JT  - Molecular genetics and metabolism
JID - 9805456
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
RN  - AE28F7PNPL (Methionine)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.40 (bleomycin hydrolase)
RN  - Homocysteinemia
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cysteine Endopeptidases/*deficiency/genetics/metabolism
MH  - Diet
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Gene Expression Regulation, Enzymologic
MH  - Genotype
MH  - Hyperhomocysteinemia/*enzymology/genetics/pathology
MH  - Isoelectric Focusing
MH  - Kidney/*metabolism
MH  - Kidney Diseases/*enzymology/*pathology
MH  - Methionine
MH  - Mice, Inbred C57BL
MH  - Protein Isoforms/metabolism
MH  - Proteins/*metabolism
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Bleomycin hydrolase
OT  - Blmh-knockout mouse
OT  - Dietary hyperhomocysteinemia
OT  - High-methionine diet
OT  - Kidney disease
OT  - Mouse kidney proteome
EDAT- 2014/06/11 06:00
MHDA- 2015/04/04 06:00
CRDT- 2014/06/11 06:00
PHST- 2014/04/10 00:00 [received]
PHST- 2014/05/18 00:00 [revised]
PHST- 2014/05/18 00:00 [accepted]
PHST- 2014/06/11 06:00 [entrez]
PHST- 2014/06/11 06:00 [pubmed]
PHST- 2015/04/04 06:00 [medline]
AID - S1096-7192(14)00172-3 [pii]
AID - 10.1016/j.ymgme.2014.05.010 [doi]
PST - ppublish
SO  - Mol Genet Metab. 2014 Aug;112(4):339-46. doi: 10.1016/j.ymgme.2014.05.010. Epub 
      2014 May 24.