PMID- 24914312
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140610
LR  - 20211021
IS  - 0973-1296 (Print)
IS  - 0976-4062 (Electronic)
IS  - 0973-1296 (Linking)
VI  - 10
IP  - Suppl 1
DP  - 2014 Jan
TI  - Simultaneous quantification of nine major active components in traditional 
      Chinese prescription Mahuang decoction and the influence of herbal compatibility 
      on their contents.
PG  - S72-9
LID - 10.4103/0973-1296.127346 [doi]
AB  - BACKGROUND: Mahuang decoction (MHD), a famous classic traditional Chinese 
      formula, has been extensively applied for treating cold, influenza, asthma, acute 
      bronchitis, and other pulmonary diseases. However, the interaction among four 
      drugs of MHD has not been clearly deciphered from the aspect of molecular 
      composition. OBJECTIVE: To assess the quality of MHD and explore the interplay 
      among different prescription drugs. MATERIALS AND METHODS: A reversed-phase high 
      performance liquid chromatography (RP-HPLC) coupled with diode array detector 
      (DAD) method for the simultaneous separation and determination of nine bioactive 
      components was developed. A somatomedin A (SMA)-phenyl column (4.6 mm x 250 mm, 5 
      mum) was eluted by a gradient mobile phase contained acetonitrile and 0.05% formic 
      acid-0.05% triethylamine aqueous solution. Four detection wavelengths (210, 252, 
      278, and 291 nm) were utilized for the quantitative analysis due to the different 
      ultraviolet (UV) spectra of these compounds. RESULTS: Satisfactory separation was 
      obtained for all the components, and the assay was fully validated in respects of 
      linearity, precision, stability, and accuracy. It was found that the calibration 
      curves for all analytes showed good linearity (R (2)>/= 0.9991) within the test 
      ranges. The relative standard deviations (RSDs) for intra- and interday 
      repeatability were not more than 1.70 and 2.66%, respectively. The spike 
      recoveries of nine components varied from 97.50 +/- 1.69 to 99.27 +/- 1.37%. 
      CONCLUSION: The established method was successfully applied to analyze nine 
      active compounds in decoction samples of various drug compatibilities of MHD. The 
      variations of contents were obvious for different combinations, which hinted the 
      mutual promotion or inhibition of componential dissolution among four herbs of 
      MHD.
FAU - He, Yu
AU  - He Y
AD  - Department of Medicinal Chemistry, College of Pharmaceutical Science, Zhejiang 
      Chinese Medical University, Hangzhou, People's Republic of China.
FAU - Zhu, Ying
AU  - Zhu Y
AD  - Department of Medicinal Chemistry, College of Pharmaceutical Science, Zhejiang 
      Chinese Medical University, Hangzhou, People's Republic of China.
FAU - Zhang, Ruping
AU  - Zhang R
AD  - Department of Medicinal Chemistry, College of Pharmaceutical Science, Zhejiang 
      Chinese Medical University, Hangzhou, People's Republic of China.
FAU - Ge, Lijun
AU  - Ge L
AD  - College of Bioengineering, Zhejiang Chinese Medical University, Hangzhou, 
      People's Republic of China.
FAU - Wan, Haitong
AU  - Wan H
AD  - College of Bioengineering, Zhejiang Chinese Medical University, Hangzhou, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pharmacogn Mag
JT  - Pharmacognosy magazine
JID - 101300403
PMC - PMC4047573
OTO - NOTNLM
OT  - Determination
OT  - HPLC-DAD
OT  - Mahuang decoction
OT  - herbal compatibility
OT  - traditional Chinese prescription
COIS- Conflict of Interest: None declared.
EDAT- 2014/06/11 06:00
MHDA- 2014/06/11 06:01
PMCR- 2014/01/01
CRDT- 2014/06/11 06:00
PHST- 2013/04/13 00:00 [received]
PHST- 2013/05/16 00:00 [revised]
PHST- 2014/02/21 00:00 [accepted]
PHST- 2014/06/11 06:00 [entrez]
PHST- 2014/06/11 06:00 [pubmed]
PHST- 2014/06/11 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - PM-10-72 [pii]
AID - 10.4103/0973-1296.127346 [doi]
PST - ppublish
SO  - Pharmacogn Mag. 2014 Jan;10(Suppl 1):S72-9. doi: 10.4103/0973-1296.127346.