PMID- 24915147
OWN - NLM
STAT- MEDLINE
DCOM- 20150129
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 6
DP  - 2014
TI  - Accumulation of BDCA1(+) dendritic cells in interstitial fibrotic lung diseases and 
      Th2-high asthma.
PG  - e99084
LID - 10.1371/journal.pone.0099084 [doi]
LID - e99084
AB  - Dendritic cells (DCs) significantly contribute to the pathology of several mouse 
      lung disease models. However, little is known of the contribution of DCs to human 
      lung diseases. In this study, we examined infiltration with BDCA1(+) DCs of human 
      lungs in patients with interstitial lung diseases or asthma. Using flow 
      cytometry, we found that these DCs increased by 5 approximately 6 fold in the lungs of patients 
      with idiopathic pulmonary fibrosis or hypersensitivity pneumonitis, which are 
      both characterized by extensive fibrosis in parenchyma. The same DC subset also 
      significantly increased in the lung parenchyma of patients with chronic 
      obstructive pulmonary disease, although the degree of increase was relatively 
      modest. By employing immunofluorescence microscopy using FcepsilonRI and MHCII as the 
      specific markers for BDCA1(+) DCs, we found that the numbers of BDCA1(+) DCs also 
      significantly increased in the airway epithelium of Th2 inflammation-associated 
      asthma. These findings suggest a potential contribution of BDCA1(+) DCs in human 
      lung diseases associated with interstitial fibrosis or Th2 airway inflammation.
FAU - Greer, Alexandra M
AU  - Greer AM
AD  - Department of Microbiology and Immunology, Sandler Asthma Basic Research Center, 
      University of California San Francisco, San Francisco, California, United States 
      of America.
FAU - Matthay, Michael A
AU  - Matthay MA
AD  - Department of Medicine and Anesthesia, University of California San Francisco, 
      San Francisco, California, United States of America.
FAU - Kukreja, Jasleen
AU  - Kukreja J
AD  - Division of Cardiothoracic Surgery, University of California San Francisco, San 
      Francisco, California, United States of America.
FAU - Bhakta, Nirav R
AU  - Bhakta NR
AD  - Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of 
      California San Francisco, San Francisco, California, United States of America.
FAU - Nguyen, Christine P
AU  - Nguyen CP
AD  - Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of 
      California San Francisco, San Francisco, California, United States of America.
FAU - Wolters, Paul J
AU  - Wolters PJ
AD  - Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of 
      California San Francisco, San Francisco, California, United States of America.
FAU - Woodruff, Prescott G
AU  - Woodruff PG
AD  - Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of 
      California San Francisco, San Francisco, California, United States of America.
FAU - Fahy, John V
AU  - Fahy JV
AD  - Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of 
      California San Francisco, San Francisco, California, United States of America.
FAU - Shin, Jeoung-Sook
AU  - Shin JS
AD  - Department of Microbiology and Immunology, Sandler Asthma Basic Research Center, 
      University of California San Francisco, San Francisco, California, United States 
      of America.
LA  - eng
GR  - T32 5T32HL007185/HL/NHLBI NIH HHS/United States
GR  - T32 AI007334/AI/NIAID NIH HHS/United States
GR  - T32 HL007185/HL/NHLBI NIH HHS/United States
GR  - P01 HL107202/HL/NHLBI NIH HHS/United States
GR  - F32 1F32HL110720/HL/NHLBI NIH HHS/United States
GR  - R01 HL051854/HL/NHLBI NIH HHS/United States
GR  - F32 HL110720/HL/NHLBI NIH HHS/United States
GR  - HL095372/HL/NHLBI NIH HHS/United States
GR  - HL51854/HL/NHLBI NIH HHS/United States
GR  - R01 HL095372/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140610
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (FcepsilonRI alpha-chain, human)
RN  - 0 (Receptors, IgE)
SB  - IM
MH  - Animals
MH  - Asthma/*immunology/pathology
MH  - Cell Count
MH  - Cohort Studies
MH  - Dendritic Cells/*immunology
MH  - Epithelium/pathology
MH  - Female
MH  - Humans
MH  - Idiopathic Pulmonary Fibrosis/*immunology/*pathology
MH  - Lung/immunology/pathology
MH  - Lung Diseases, Interstitial/*immunology/*pathology
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Receptors, IgE/metabolism
MH  - Th2 Cells/*immunology
PMC - PMC4051692
COIS- Competing Interests: The authors have read the journal's policy and have the 
      following conflicts: PGW has received research grants from Pfizer and Genentech, 
      serves as a consultant for Medimmune, Genentech, Merck, Boehringer-Ingelheim, 
      Kalobios. This does not alter adherence to all the PLOS ONE policies on sharing 
      data and materials.
EDAT- 2014/06/11 06:00
MHDA- 2015/01/30 06:00
PMCR- 2014/06/10
CRDT- 2014/06/11 06:00
PHST- 2013/07/03 00:00 [received]
PHST- 2014/04/09 00:00 [accepted]
PHST- 2014/06/11 06:00 [entrez]
PHST- 2014/06/11 06:00 [pubmed]
PHST- 2015/01/30 06:00 [medline]
PHST- 2014/06/10 00:00 [pmc-release]
AID - PONE-D-13-27363 [pii]
AID - 10.1371/journal.pone.0099084 [doi]
PST - epublish
SO  - PLoS One. 2014 Jun 10;9(6):e99084. doi: 10.1371/journal.pone.0099084. eCollection 
      2014.