PMID- 24915541
OWN - NLM
STAT- MEDLINE
DCOM- 20150129
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 6
DP  - 2014
TI  - Intracellular bacteria interfere with dendritic cell functions: role of the type 
      I interferon pathway.
PG  - e99420
LID - 10.1371/journal.pone.0099420 [doi]
LID - e99420
AB  - Dendritic cells (DCs) orchestrate host defenses against microorganisms. In 
      infectious diseases due to intracellular bacteria, the inefficiency of the immune 
      system to eradicate microorganisms has been attributed to the hijacking of DC 
      functions. In this study, we selected intracellular bacterial pathogens with 
      distinct lifestyles and explored the responses of monocyte-derived DCs (moDCs). 
      Using lipopolysaccharide as a control, we found that Orientia tsutsugamushi, the 
      causative agent of scrub typhus that survives in the cytosol of target cells, 
      induced moDC maturation, as assessed by decreased endocytosis activity, the 
      ability to induce lymphocyte proliferation and the membrane expression of 
      phenotypic markers. In contrast, Coxiella burnetii, the agent of Q fever, and 
      Brucella abortus, the agent of brucellosis, both of which reside in vacuolar 
      compartments, only partly induced the maturation of moDCs, as demonstrated by a 
      phenotypic analysis. To analyze the mechanisms used by C. burnetii and B. abortus 
      to alter moDC activation, we performed microarray and found that C. burnetii and 
      B. abortus induced a specific signature consisting of TLR4, TLR3, STAT1 and 
      interferon response genes. These genes were down-modulated in response to C. 
      burnetii and B. abortus but up-modulated in moDCs activated by lipopolysaccharide 
      and O. tsutsugamushi. This transcriptional alteration was associated with the 
      defective interferon-beta production. This study demonstrates that intracellular 
      bacteria specifically affect moDC responses and emphasizes how C. burnetii and B. 
      abortus interfere with moDC activation and the antimicrobial immune response. We 
      believe that comparing infection by several bacterial species may be useful for 
      defining new pathways and biomarkers and for developing new treatment strategies.
FAU - Gorvel, Laurent
AU  - Gorvel L
AD  - Centre National de la Recherche Scientifique UMR 7278, IRD198, INSERM U1095, 
      Aix-Marseille Universite, Marseille, France.
FAU - Textoris, Julien
AU  - Textoris J
AD  - Centre National de la Recherche Scientifique UMR 7278, IRD198, INSERM U1095, 
      Aix-Marseille Universite, Marseille, France.
FAU - Banchereau, Romain
AU  - Banchereau R
AD  - Baylor Institute for Immunology Research, Dallas, Texas, United States of 
      America.
FAU - Ben Amara, Amira
AU  - Ben Amara A
AD  - Centre National de la Recherche Scientifique UMR 7278, IRD198, INSERM U1095, 
      Aix-Marseille Universite, Marseille, France.
FAU - Tantibhedhyangkul, Wiwit
AU  - Tantibhedhyangkul W
AD  - Centre National de la Recherche Scientifique UMR 7278, IRD198, INSERM U1095, 
      Aix-Marseille Universite, Marseille, France; Department of Immunology, Faculty of 
      Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - von Bargen, Kristin
AU  - von Bargen K
AD  - Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 
      INSERM, U1104, CNRS, UMR7280, Marseille, France.
FAU - Ka, Mignane B
AU  - Ka MB
AD  - Centre National de la Recherche Scientifique UMR 7278, IRD198, INSERM U1095, 
      Aix-Marseille Universite, Marseille, France.
FAU - Capo, Christian
AU  - Capo C
AD  - Centre National de la Recherche Scientifique UMR 7278, IRD198, INSERM U1095, 
      Aix-Marseille Universite, Marseille, France.
FAU - Ghigo, Eric
AU  - Ghigo E
AD  - Centre National de la Recherche Scientifique UMR 7278, IRD198, INSERM U1095, 
      Aix-Marseille Universite, Marseille, France.
FAU - Gorvel, Jean-Pierre
AU  - Gorvel JP
AD  - Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 
      INSERM, U1104, CNRS, UMR7280, Marseille, France.
FAU - Mege, Jean-Louis
AU  - Mege JL
AD  - Centre National de la Recherche Scientifique UMR 7278, IRD198, INSERM U1095, 
      Aix-Marseille Universite, Marseille, France.
LA  - eng
PT  - Journal Article
DEP - 20140610
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 77238-31-4 (Interferon-beta)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Brucella abortus/*physiology
MH  - Cell Differentiation
MH  - Coxiella burnetii/*physiology
MH  - Dendritic Cells/*immunology
MH  - Gene Expression Profiling
MH  - Humans
MH  - Interferon-beta/metabolism
MH  - Intracellular Space/*microbiology
MH  - Mice, Inbred BALB C
MH  - Monocytes/cytology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phenotype
MH  - Phosphorylation
MH  - *Signal Transduction
MH  - Transcription, Genetic
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
PMC - PMC4051653
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/06/11 06:00
MHDA- 2015/01/30 06:00
PMCR- 2014/06/10
CRDT- 2014/06/11 06:00
PHST- 2014/01/28 00:00 [received]
PHST- 2014/05/14 00:00 [accepted]
PHST- 2014/06/11 06:00 [entrez]
PHST- 2014/06/11 06:00 [pubmed]
PHST- 2015/01/30 06:00 [medline]
PHST- 2014/06/10 00:00 [pmc-release]
AID - PONE-D-14-03256 [pii]
AID - 10.1371/journal.pone.0099420 [doi]
PST - epublish
SO  - PLoS One. 2014 Jun 10;9(6):e99420. doi: 10.1371/journal.pone.0099420. eCollection 
      2014.