PMID- 24915811
OWN - NLM
STAT- MEDLINE
DCOM- 20141201
LR  - 20160818
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 43
IP  - 4
DP  - 2014 Apr
TI  - [Assessment of HER2 gene amplification in breast cancer: a comparison of 
      dual-color in-situ hybridization and fluorescence in-situ hybridization].
PG  - 226-30
AB  - OBJECTIVE: To compare dual-color in-situ hybridization (DISH) with fluorescence 
      in situ hybridization(FISH) in evaluating the human HER2 gene status in invasive 
      breast cancer. METHODS: HER2 gene status in 110 cases of breast invasive ductal 
      carcinomas with a 2+ score on immunohistochemistry (IHC) was investigated by FISH 
      and DISH. The 2007 and 2013 ASCO (American Society of Clinical Oncology)/CAP 
      (College of American Pathologists) HER2 guideline were used respectively to 
      evaluate the agreement between these two techniques. RESULTS: (1) Using the 2007 
      ASCO/CAP guideline, the overall concordance between FISH and DISH was 97.3% 
      (107/110). Fifty of 51 samples with amplification by FISH were also detected by 
      DISH and the remaining one sample was equivocal.Eight of 10 equivocal samples by 
      FISH were equivocal by DISH and the remaining two samples were negative. 
      Forty-nine samples with no amplification by FISH were all negative by DISH. The 
      concordance was 98.0%, 8/10 and 100.0% respectively for the FISH positive, 
      equivocal and negative groups. (2) Using the 2013 ASCO/CAP guideline, the overall 
      concordance between FISH and DISH was 90.0% (99/110). Fifty-three of 55 samples 
      with amplification by FISH were also detected by DISH and the remaining two were 
      equivocal and negative respectively. Two of 12 equivocal samples by FISH were 
      equivocal by DISH and the remaining ten samples were negative in 7 cases and 
      equivocal in 3 cases. Forty-three samples with no amplification by FISH were all 
      negative by DISH. The concordance was 96.4%, 3/12 and 100.0% respectively for the 
      FISH positive, equivocal and negative groups. Pearson correlation analysis 
      indicated that the HER2 status detected by FISH and DISH were significantly 
      correlated with each other (R=0.584, P<0.01). CONCLUSIONS: There is a high 
      concordance between DISH and FISH for assessing the HER2 gene status in invasive 
      breast cancer. DISH is a new option for assessing HER2 gene status of breast 
      cancer in clinical practice. The clinical significance of the discordance between 
      DISH and FISH in equivocal cases warrants further study.
FAU - Xu, Yan
AU  - Xu Y
AD  - Department of Pathology, Cancer Hospital, Fudan University and Department of 
      Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
FAU - Yang, Wentao
AU  - Yang W
AD  - Department of Pathology, Cancer Hospital, Fudan University and Department of 
      Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
FAU - Yang, Fei
AU  - Yang F
AD  - Department of Pathology, Cancer Hospital, Fudan University and Department of 
      Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
FAU - Lu, Yongming
AU  - Lu Y
AD  - Department of Pathology, Cancer Hospital, Fudan University and Department of 
      Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
FAU - Cai, Xu
AU  - Cai X
AD  - Department of Pathology, Cancer Hospital, Fudan University and Department of 
      Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
FAU - Zhou, Xiaoyan
AU  - Zhou X
AD  - Department of Pathology, Cancer Hospital, Fudan University and Department of 
      Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. 
      E-mail:xyzhou100@163.com.
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Carcinoma, Ductal, Breast/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - *Genes, erbB-2
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/methods
EDAT- 2014/06/12 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/06/12 06:00
PHST- 2014/06/12 06:00 [entrez]
PHST- 2014/06/12 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2014 Apr;43(4):226-30.