PMID- 24917652
OWN - NLM
STAT- MEDLINE
DCOM- 20151116
LR  - 20191210
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 34
IP  - 2
DP  - 2015 Feb
TI  - Nuclear factor erythroid 2-related factor 2 rescues the oxidative stress induced 
      by di-N-butylphthalate in testicular Leydig cells.
PG  - 145-52
LID - 10.1177/0960327114530744 [doi]
AB  - AIM: This study aimed to determine whether nuclear factor erythroid 2-related 
      factor 2 antagonized the oxidative stress induced by di-N-butylphthalate (DBP) in 
      testicular Leydig cells. METHODS: Mouse TM3 testicular Leydig cells were treated 
      with Nrf2 knockdown (KD) or overexpression in the presence and absence of DBP. 
      Oxidative profiles were examined. Nrf2 target antioxidant genes were studied, and 
      the effects of Nrf2 inducer sulphoraphane (SFN) were tested. RESULTS: DBP induced 
      intracellular oxidative stress to a similar extent with Nrf2 KD. Expression and 
      protein levels of Nrf2 were increased together with its target genes, namely heme 
      oxygenase 1, nicotinamide adenine dinucleotide phosphate quinone oxidoreductase 1 
      and peroxiredoxin 6, following DBP stimulation. Use of SFN not only restored the 
      intracellular oxidative toxicity but also cell proliferation and testosterone 
      secretion in response to DBP. CONCLUSION: Increased Nrf2 activity, for example, 
      by SFN can effectively antagonize the oxidative stress in testicular Leydig cells 
      caused by DBP.
CI  - (c) The Author(s) 2015.
FAU - Shen, B
AU  - Shen B
AD  - Department of Urology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China.
FAU - Wang, W
AU  - Wang W
AD  - Department of Urology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China.
FAU - Ding, L
AU  - Ding L
AD  - Department of Urology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China.
FAU - Sao, Y
AU  - Sao Y
AD  - Department of Urology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China.
FAU - Huang, Y
AU  - Huang Y
AD  - Department of Urology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China.
FAU - Shen, Z
AU  - Shen Z
AD  - Department of Urology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China.
FAU - Zhuo, Y
AU  - Zhuo Y
AD  - Department of Urology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China.
FAU - Wei, Z
AU  - Wei Z
AD  - Department of Urology, The Second Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China.
FAU - Zhang, W
AU  - Zhang W
AD  - Department of Urology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, People's Republic of China dravanti@163.com wzq@nmu.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140610
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Environmental Pollutants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Reactive Oxygen Species)
RN  - 2286E5R2KE (Dibutyl Phthalate)
RN  - 3XMK78S47O (Testosterone)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - G9481N71RO (Deoxyguanosine)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Animals
MH  - Cell Line
MH  - Deoxyguanosine/analogs & derivatives/metabolism
MH  - Dibutyl Phthalate/*toxicity
MH  - Endocrine Disruptors/*toxicity
MH  - Environmental Pollutants/*toxicity
MH  - Gene Knockdown Techniques
MH  - Glutathione/metabolism
MH  - Lentivirus/genetics
MH  - Leydig Cells/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - NF-E2-Related Factor 2/genetics/*metabolism
MH  - Oxidative Stress/*drug effects
MH  - RNA, Small Interfering/genetics
MH  - Reactive Oxygen Species/metabolism
MH  - Testosterone/metabolism
OTO - NOTNLM
OT  - Di-N-butylphthalate
OT  - Nrf2
OT  - oxidative stress
OT  - testicular Leydig cells
EDAT- 2014/06/12 06:00
MHDA- 2015/11/17 06:00
CRDT- 2014/06/12 06:00
PHST- 2014/06/12 06:00 [entrez]
PHST- 2014/06/12 06:00 [pubmed]
PHST- 2015/11/17 06:00 [medline]
AID - 0960327114530744 [pii]
AID - 10.1177/0960327114530744 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2015 Feb;34(2):145-52. doi: 10.1177/0960327114530744. Epub 2014 
      Jun 10.