PMID- 24919066
OWN - NLM
STAT- MEDLINE
DCOM- 20150202
LR  - 20240314
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 6
DP  - 2014
TI  - Meiotic recombination analyses of individual chromosomes in male domestic pigs 
      (Sus scrofa domestica).
PG  - e99123
LID - 10.1371/journal.pone.0099123 [doi]
LID - e99123
AB  - For the first time in the domestic pig, meiotic recombination along the 18 
      porcine autosomes was directly studied by immunolocalization of MLH1 protein. In 
      total, 7,848 synaptonemal complexes from 436 spermatocytes were analyzed, and 
      13,969 recombination sites were mapped. Individual chromosomes for 113 of the 436 
      cells (representing 2,034 synaptonemal complexes) were identified by 
      immunostaining and fluorescence in situ hybridization (FISH). The average total 
      length of autosomal synaptonemal complexes per cell was 190.3 microm, with 32.0 
      recombination sites (crossovers), on average, per cell. The number of crossovers 
      and the lengths of the autosomal synaptonemal complexes showed significant intra- 
      (i.e. between cells) and inter-individual variations. The distributions of 
      recombination sites within each chromosomal category were similar: crossovers in 
      metacentric and submetacentric chromosomes were concentrated in the telomeric 
      regions of the p- and q-arms, whereas two hotspots were located near the 
      centromere and in the telomeric region of acrocentrics. Lack of MLH1 foci was 
      mainly observed in the smaller chromosomes, particularly chromosome 18 (SSC18) 
      and the sex chromosomes. All autosomes displayed positive interference, with a 
      large variability between the chromosomes.
FAU - Mary, Nicolas
AU  - Mary N
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
FAU - Barasc, Harmonie
AU  - Barasc H
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
FAU - Ferchaud, Stephane
AU  - Ferchaud S
AD  - UE1372 GenESI Genetique, Experimentation et Systeme Innovants, Surgeres, France.
FAU - Billon, Yvon
AU  - Billon Y
AD  - UE1372 GenESI Genetique, Experimentation et Systeme Innovants, Surgeres, France.
FAU - Meslier, Frederic
AU  - Meslier F
AD  - UE1372 GenESI Genetique, Experimentation et Systeme Innovants, Surgeres, France.
FAU - Robelin, David
AU  - Robelin D
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
FAU - Calgaro, Anne
AU  - Calgaro A
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
FAU - Loustau-Dudez, Anne-Marie
AU  - Loustau-Dudez AM
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
FAU - Bonnet, Nathalie
AU  - Bonnet N
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
FAU - Yerle, Martine
AU  - Yerle M
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
FAU - Acloque, Herve
AU  - Acloque H
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
FAU - Ducos, Alain
AU  - Ducos A
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
FAU - Pinton, Alain
AU  - Pinton A
AD  - INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage, Castanet-Tolosan, 
      France; Universite de Toulouse INPT ENSAT, UMR1388 Genetique, Physiologie et 
      Systemes d'Elevage, Castanet-Tolosan, France; Universite de Toulouse INPT ENVT, 
      UMR1388 Genetique, Physiologie et Systemes d'Elevage, Toulouse, France.
LA  - eng
PT  - Journal Article
DEP - 20140611
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - *Chromosomes
MH  - Male
MH  - *Meiosis
MH  - *Recombination, Genetic
MH  - Swine/*genetics
PMC - PMC4053413
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/06/12 06:00
MHDA- 2015/02/03 06:00
PMCR- 2014/06/11
CRDT- 2014/06/12 06:00
PHST- 2014/03/19 00:00 [received]
PHST- 2014/05/09 00:00 [accepted]
PHST- 2014/06/12 06:00 [entrez]
PHST- 2014/06/12 06:00 [pubmed]
PHST- 2015/02/03 06:00 [medline]
PHST- 2014/06/11 00:00 [pmc-release]
AID - PONE-D-14-12344 [pii]
AID - 10.1371/journal.pone.0099123 [doi]
PST - epublish
SO  - PLoS One. 2014 Jun 11;9(6):e99123. doi: 10.1371/journal.pone.0099123. eCollection 
      2014.