PMID- 24919714
OWN - NLM
STAT- MEDLINE
DCOM- 20150721
LR  - 20181202
IS  - 1743-1328 (Electronic)
IS  - 0161-6412 (Linking)
VI  - 37
IP  - 1
DP  - 2015 Jan
TI  - Transplanted hUCB-MSCs migrated to the damaged area by SDF-1/CXCR4 signaling to 
      promote functional recovery after traumatic brain injury in rats.
PG  - 50-6
LID - 10.1179/1743132814Y.0000000399 [doi]
AB  - Transplanted human umbilical cord mesenchymal stem cells (hUC-MSCs) have 
      exhibited considerable therapeutic potential for traumatic brain injury (TBI). 
      However, how hUC-MSCs migrating to the injury region and the mechanism of 
      hUC-MSCs promoting functional recovery after TBI are still unclear. In this 
      study, we investigated whether stromal cell-derived factor-1 (SDF-1) was involved 
      in the hUC-MSCs migration and the possible mechanisms that might be involved in 
      the beneficial effect on functional recovery. In vitro experiments demonstrated 
      that SDF-1 induces a concentration-dependent migration of hUC-MSCs. Furthermore, 
      pre-treatment with the CXCR4-specific antagonist AMD3100 significantly prevented 
      the migration of hUC-MSCs in vitro. We found that the expression of SDF-1 
      increased significantly around the damaged area. Transplanted hUC-MSCs were 
      localized to regions where SDF-1 was highly expressed. Additionally, our results 
      showed that hUC-MSCs-treated animals showed significantly improved functional 
      recovery compared with controls. In hUC-MSCs-transplanted group, terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive 
      cells were decreased and BrdU-positive cells were significantly increased 
      compared with control group, more of BrdU-positive cells co-localized with GFAP. 
      These suggest that SDF-1 plays an important role in the migration of hUC-MSCs to 
      the damaged area and hUC-MSCs are beneficial for functional recovery after TBI.
FAU - Ma, Jianhua
AU  - Ma J
FAU - Liu, Ning
AU  - Liu N
FAU - Yi, Bo
AU  - Yi B
FAU - Zhang, Xiaochong
AU  - Zhang X
FAU - Gao, Bing Bing
AU  - Gao BB
FAU - Zhang, Yesen
AU  - Zhang Y
FAU - Xu, Ruxiang
AU  - Xu R
FAU - Li, Xin
AU  - Li X
FAU - Dai, Yiwu
AU  - Dai Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140612
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (CXCL12 protein, rat)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcr4 protein, rat)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Brain/drug effects/pathology/physiopathology
MH  - Brain Injuries/pathology/physiopathology/*therapy
MH  - Cell Movement/drug effects/*physiology
MH  - Cells, Cultured
MH  - Chemokine CXCL12/*metabolism
MH  - Disease Models, Animal
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/drug effects/pathology/*physiology
MH  - Neurogenesis/physiology
MH  - RNA, Messenger
MH  - Rats, Sprague-Dawley
MH  - Receptors, CXCR4/antagonists & inhibitors/*metabolism
MH  - Recovery of Function/physiology
MH  - Signal Transduction
MH  - Treatment Outcome
OTO - NOTNLM
OT  - SDF-1/CXCR4
OT  - Traumatic brain injury,
OT  - hUCB-MSCs,
EDAT- 2014/06/13 06:00
MHDA- 2015/07/22 06:00
CRDT- 2014/06/13 06:00
PHST- 2014/06/13 06:00 [entrez]
PHST- 2014/06/13 06:00 [pubmed]
PHST- 2015/07/22 06:00 [medline]
AID - 10.1179/1743132814Y.0000000399 [doi]
PST - ppublish
SO  - Neurol Res. 2015 Jan;37(1):50-6. doi: 10.1179/1743132814Y.0000000399. Epub 2014 
      Jun 12.