PMID- 24920235
OWN - NLM
STAT- MEDLINE
DCOM- 20150407
LR  - 20220318
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Linking)
VI  - 52
IP  - 7
DP  - 2014 Jul
TI  - Caffeic acid phenethyl ester promotes anti-inflammatory effects by inhibiting 
      MAPK and NF-kappaB signaling in activated HMC-1 human mast cells.
PG  - 926-32
LID - 10.3109/13880209.2013.865243 [doi]
AB  - CONTEXT: Caffeic acid phenethyl ester (CAPE), an active component of honeybee 
      propolis, is known to have antioxidant, anti-inflammatory, and other beneficial 
      medicinal properties. However, the molecular mechanisms underlying its 
      anti-allergic effects in mast cells are unknown. OBJECTIVE: The purpose of the 
      present study was to examine whether CAPE modulates the immunoglobulin E 
      (IgE)-mediated local allergic reaction in animals, as well as to elucidate the 
      effects of CAPE on mast cells in vitro. MATERIALS AND METHODS: To investigate the 
      bioactive potential of CAPE (10 or 20 microM), HMC-1 cells were stimulated with 
      phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI) for 24 h in 
      the presence or absence of CAPE. To study the pharmacological effects of CAPE, 
      enzyme-linked immunosorbent assays (ELISAs), RT-PCR, Western blot analysis, 
      electrophoretic mobility shift assays (EMSAs), and fluorescence assays were used. 
      RESULTS: CAPE (10 mg/kg) inhibited local IgE-mediated allergic reactions (0.164 
      versus 0.065 O.D.) in a mouse model. Additionally, CAPE (20 microM) attenuated 
      PMACI-stimulated histamine release (3146.42 versus 2564.83 pg/ml) and the 
      production of inflammatory cytokines, such as interleukin (IL)-1beta (4.775 versus 
      0.713 pg/ml, IC50 = 6.67 microM), IL-6 (4771.5 versus 449.1 pg/ml, IC50 = 5.25 microM), 
      and IL-8 (5991.7 versus 2213.1 pg/ml, IC50 = 9.95 microM) in HMC-1 cells. In 
      activated HMC-1 cells, pretreatment with CAPE decreased the phosphorylation of 
      c-Jun N-terminal kinase. In addition, CAPE inhibited PMACI-induced nuclear factor 
      (NF)-kappaB activation by suppressing IkappaBalpha phosphorylation and its degradation. 
      DISCUSSION AND CONCLUSION: Our results indicated that CAPE can modulate mast 
      cell-mediated allergic disease.
FAU - Cho, Mi Suk
AU  - Cho MS
AD  - Department of Dental Hygiene, Choonhae College of Health Sciences , Ulsan , 
      Republic of Korea .
FAU - Park, Won Sun
AU  - Park WS
FAU - Jung, Won-Kyo
AU  - Jung WK
FAU - Qian, Zhong-Ji
AU  - Qian ZJ
FAU - Lee, Dae-Sung
AU  - Lee DS
FAU - Choi, Jung-Sik
AU  - Choi JS
FAU - Lee, Da-Young
AU  - Lee DY
FAU - Park, Sae-Gwang
AU  - Park SG
FAU - Seo, Su-Kil
AU  - Seo SK
FAU - Kim, Hak-Ju
AU  - Kim HJ
FAU - Won, Jun Yeon
AU  - Won JY
FAU - Yu, Byeng Chul
AU  - Yu BC
FAU - Choi, Il-Whan
AU  - Choi IW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Caffeic Acids)
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - G960R9S5SK (caffeic acid phenethyl ester)
RN  - ML9LGA7468 (Phenylethyl Alcohol)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - Caffeic Acids/*pharmacology
MH  - Calcium/metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cytokines/drug effects
MH  - Histamine/metabolism
MH  - Histamine Release/drug effects
MH  - Humans
MH  - Hypersensitivity/prevention & control
MH  - Immunoglobulin E/metabolism
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - MAP Kinase Signaling System/*drug effects
MH  - Male
MH  - Mast Cells/cytology/*drug effects/metabolism
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/*antagonists & inhibitors/metabolism
MH  - NF-kappa B/*antagonists & inhibitors/metabolism
MH  - Phenylethyl Alcohol/*analogs & derivatives/pharmacology
MH  - Phosphorylation/drug effects
OTO - NOTNLM
OT  - Allergy
OT  - cytokine
OT  - histamine
OT  - immunoglobulin E
OT  - passive cutaneous anaphylaxis
EDAT- 2014/06/13 06:00
MHDA- 2015/04/08 06:00
CRDT- 2014/06/13 06:00
PHST- 2014/06/13 06:00 [entrez]
PHST- 2014/06/13 06:00 [pubmed]
PHST- 2015/04/08 06:00 [medline]
AID - 10.3109/13880209.2013.865243 [doi]
PST - ppublish
SO  - Pharm Biol. 2014 Jul;52(7):926-32. doi: 10.3109/13880209.2013.865243.