PMID- 24921856
OWN - NLM
STAT- MEDLINE
DCOM- 20150706
LR  - 20211021
IS  - 1528-1159 (Electronic)
IS  - 0362-2436 (Print)
IS  - 0362-2436 (Linking)
VI  - 39
IP  - 19
DP  - 2014 Sep 1
TI  - Upregulation of BDNF and NGF in cervical intervertebral discs exposed to painful 
      whole-body vibration.
PG  - 1542-8
LID - 10.1097/BRS.0000000000000457 [doi]
AB  - STUDY DESIGN: In vivo study defining expression of the neurotrophins, 
      brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), in 
      cervical intervertebral discs after painful whole-body vibration (WBV). 
      OBJECTIVE: The goal of this study is to determine if BDNF and NGF are expressed 
      in cervical discs after painful WBV in a rat model. SUMMARY OF BACKGROUND DATA: 
      WBV is a possible source of neck pain and has been implicated as increasing the 
      risk for disc disorders. Typically, aneural regions of painful human lumbar discs 
      exhibit hyperinnervation, suggesting nerve ingrowth as potentially contributing 
      to disc degeneration and pain. BDNF and NGF are upregulated in painfully 
      degenerate lumbar discs and hypothesized to contribute to this pathology. 
      METHODS: Male Holtzman rats underwent 7 days of repeated WBV (15 Hz, 30 min/d) or 
      sham exposures, followed by 7 days of rest. Cervical discs were collected for 
      analysis of BDNF and NGF expression through RT-qPCR and Western blot analysis. 
      Immunohistochemistry also evaluated their regional expression in the disc. 
      RESULTS: Vibration significantly increases BDNF messenger ribonucleic acid (mRNA) 
      levels (P=0.036), as well as total-NGF mRNA (P=0.035). Protein expression of both 
      BDNF (P=0.006) and the 75-kDa NGF (P=0.045) increase by nearly 4- and 10-fold, 
      respectively. Both BDNF mRNA (R=0.396; P=0.012) and protein (R=0.280; P=0.035) 
      levels are significantly correlated with the degree of behavioral sensitivity 
      (i.e., pain) at day 14. Total-NGF mRNA is also significantly correlated with the 
      extent of behavioral sensitivity (R=0.276; P=0.044). Both neurotrophins are most 
      increased in the inner annulus fibrosus and nucleus pulposus. CONCLUSION: The 
      increases in BDNF and NGF in the cervical discs after painful vibration are 
      observed in typically aneural regions of the disc, consistent with reports of its 
      hyperinnervation. Yet, the induction of nerve ingrowth into the disc was not 
      explicitly investigated. Neurotrophin expression also correlates with behavioral 
      sensitivity, suggesting a role for both neurotrophins in the development of disc 
      pain. LEVEL OF EVIDENCE: N/A.
FAU - Kartha, Sonia
AU  - Kartha S
AD  - Departments of *Bioengineering and daggerNeurosurgery, University of Pennsylvania, 
      Philadelphia, PA.
FAU - Zeeman, Martha E
AU  - Zeeman ME
FAU - Baig, Hassam A
AU  - Baig HA
FAU - Guarino, Benjamin B
AU  - Guarino BB
FAU - Winkelstein, Beth A
AU  - Winkelstein BA
LA  - eng
GR  - R01 AR064157/AR/NIAMS NIH HHS/United States
GR  - AR064157/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Spine (Phila Pa 1976)
JT  - Spine
JID - 7610646
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Cervical Vertebrae
MH  - Cumulative Trauma Disorders/etiology/genetics/*metabolism
MH  - Intervertebral Disc/*metabolism
MH  - Male
MH  - Neck Pain/*etiology/genetics/metabolism
MH  - Nerve Growth Factor/*biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Up-Regulation
MH  - Vibration/*adverse effects
PMC - PMC4149599
MID - NIHMS599757
EDAT- 2014/06/13 06:00
MHDA- 2015/07/07 06:00
PMCR- 2015/09/01
CRDT- 2014/06/13 06:00
PHST- 2014/06/13 06:00 [entrez]
PHST- 2014/06/13 06:00 [pubmed]
PHST- 2015/07/07 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - 10.1097/BRS.0000000000000457 [doi]
PST - ppublish
SO  - Spine (Phila Pa 1976). 2014 Sep 1;39(19):1542-8. doi: 
      10.1097/BRS.0000000000000457.