PMID- 24923741
OWN - NLM
STAT- MEDLINE
DCOM- 20140902
LR  - 20211021
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 47
IP  - 4
DP  - 2014 Aug
TI  - BMP4 promotes SSEA-1(+) hUC-MSC differentiation into male germ-like cells in 
      vitro.
PG  - 299-309
LID - 10.1111/cpr.12115 [doi]
AB  - OBJECTIVES: Recent studies have demonstrated that primordial germ cells (PGC) can 
      be differentiated from human umbilical cord mesenchymal stem cells (hUC-MSCs), 
      and embryonic stem cells (ESCs) in vitro. Nevertheless, efficiencies were low and 
      unstable. Here, whether hUC-MSCs can be induced to differentiate into germ-like 
      cells with the aid of bone morphogenetic protein (BMP4) was investigated. 
      MATERIALS AND METHODS: Human umbilical cord mesenchymal stem cells were freshly 
      isolated and cultured with BMP4. SSEA-1(+/-) cells were purified using 
      magnetic-activated cell sorting (MACS) from the hUC-MSCs, and further induced 
      with BMP4. Quantitative real-time PCR (qRT-PCR) and immunofluorescence analysis 
      were used to determine PGC and germ-like cell-specific markers. RESULTS: Human 
      umbilical cord mesenchymal stem cells differentiated into SSEA-1(+) spherical 
      PGC-like cells efficiently with 12.5 ng/ml BMP4. qRT-PCR and immunofluorescence 
      analysis demonstrated that SSEA-1(+) cells expressed higher levels of 
      PGC-specific markers than SSEA-1(-) cells. Furthermore, SSEA-1(+) cells were 
      induced with BMP4 to differentiate into STRA8, SCP3, DMRT1 and PLZF-positive male 
      germ-like cells, and some sperm-like cells were obtained by 7-14 days after 
      induction. CONCLUSION: These results suggest that SSEA-1(+) hUC-MSCs can 
      differentiate into male germ-like cells in the presence of BMP4. This study 
      provides an efficient protocol to study germ-cell development using hUC-MSCs.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Li, N
AU  - Li N
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & 
      Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, 
      Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Pan, S
AU  - Pan S
FAU - Zhu, H
AU  - Zhu H
FAU - Mu, H
AU  - Mu H
FAU - Liu, W
AU  - Liu W
FAU - Hua, J
AU  - Hua J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140613
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (BMP4 protein, human)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Lewis X Antigen)
SB  - IM
MH  - Bone Morphogenetic Protein 4/*metabolism
MH  - Cells, Cultured
MH  - Humans
MH  - Lewis X Antigen/*metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - *Spermatogenesis
MH  - Umbilical Cord/*cytology
PMC - PMC6495181
EDAT- 2014/06/14 06:00
MHDA- 2014/09/03 06:00
PMCR- 2014/06/13
CRDT- 2014/06/14 06:00
PHST- 2014/01/13 00:00 [received]
PHST- 2014/03/20 00:00 [accepted]
PHST- 2014/06/14 06:00 [entrez]
PHST- 2014/06/14 06:00 [pubmed]
PHST- 2014/09/03 06:00 [medline]
PHST- 2014/06/13 00:00 [pmc-release]
AID - CPR12115 [pii]
AID - 10.1111/cpr.12115 [doi]
PST - ppublish
SO  - Cell Prolif. 2014 Aug;47(4):299-309. doi: 10.1111/cpr.12115. Epub 2014 Jun 13.