PMID- 24924836
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20211021
IS  - 1525-1470 (Electronic)
IS  - 0736-8046 (Print)
IS  - 0736-8046 (Linking)
VI  - 31
IP  - 5
DP  - 2014 Sep-Oct
TI  - Fluorescence in situ hybridization analysis of atypical melanocytic 
      proliferations and melanoma in young patients.
PG  - 561-9
LID - 10.1111/pde.12382 [doi]
AB  - Morphologic heterogeneity among melanocytic proliferations is a common challenge 
      in the diagnosis of melanoma. In particular, atypical melanocytic lesions in 
      children, adolescents, and young adults may be difficult to classify because of 
      significant morphologic overlap with melanoma. Recently a four-probe fluorescence 
      in situ hybridization (FISH) protocol to detect chromosomal abnormalities in 
      chromosomes 6 and 11 has shown promise for improving the classification of 
      melanocytic lesions. We sought to determine the correlation between FISH results, 
      morphology, and clinical outcomes in a series of challenging melanocytic 
      proliferations in young patients. We retrospectively performed the standard 
      four-probe FISH analysis on 21 melanocytic neoplasms from 21 patients younger 
      than 25 years of age (range 5-25 years, mean 14.6 years) from Stanford University 
      Medical Center who were prospectively followed for a median of 51 months (range 
      1-136 months). The study cohort included patients with 5 confirmed melanomas, 2 
      melanocytic tumors of uncertain malignant potential (MelTUMPs), 10 
      morphologically challenging atypical Spitz tumors (ASTs), and 4 typical Spitz 
      nevi. FISH detected chromosomal aberrations in all five melanomas and in one 
      MelTUMP, in which the patient developed subsequent lymph node and distant 
      metastasis. All 10 ASTs, 4 Spitz nevi, and 1 of 2 MelTUMPs were negative for 
      significant gains or losses in chromosomes 6 and 11q. Our findings demonstrated a 
      strong correlation between positive FISH results and the histomorphologic 
      impression of melanoma. This finding was also true for the MelTUMP with poor 
      clinical outcome. Therefore FISH may serve as a helpful adjunct in the 
      classification of controversial melanocytic tumors in young patients.
CI  - (c) 2014 The Authors. Pediatric Dermatology published by Wiley Periodicals, Inc.
FAU - DeMarchis, Emilia H
AU  - DeMarchis EH
AD  - School of Medicine, Stanford University, Stanford, California, USA.
FAU - Swetter, Susan M
AU  - Swetter SM
FAU - Jennings, Charay D
AU  - Jennings CD
FAU - Kim, Jinah
AU  - Kim J
LA  - eng
PT  - Journal Article
DEP - 20140613
PL  - United States
TA  - Pediatr Dermatol
JT  - Pediatric dermatology
JID - 8406799
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*diagnosis/*genetics
MH  - Nevus, Pigmented/*diagnosis/*genetics
MH  - Precancerous Conditions/*diagnosis/*genetics
MH  - Prospective Studies
MH  - Retrospective Studies
MH  - Skin Neoplasms/*diagnosis/*genetics
PMC - PMC4282368
EDAT- 2014/06/14 06:00
MHDA- 2015/05/15 06:00
PMCR- 2015/01/02
CRDT- 2014/06/14 06:00
PHST- 2014/06/14 06:00 [entrez]
PHST- 2014/06/14 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
PHST- 2015/01/02 00:00 [pmc-release]
AID - 10.1111/pde.12382 [doi]
PST - ppublish
SO  - Pediatr Dermatol. 2014 Sep-Oct;31(5):561-9. doi: 10.1111/pde.12382. Epub 2014 Jun 
      13.