PMID- 24926684
OWN - NLM
STAT- MEDLINE
DCOM- 20151026
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 6
DP  - 2014
TI  - Certain adenylated non-coding RNAs, including 5' leader sequences of primary 
      microRNA transcripts, accumulate in mouse cells following depletion of the RNA 
      helicase MTR4.
PG  - e99430
LID - 10.1371/journal.pone.0099430 [doi]
LID - e99430
AB  - RNA surveillance plays an important role in posttranscriptional regulation. 
      Seminal work in this field has largely focused on yeast as a model system, 
      whereas exploration of RNA surveillance in mammals is only recently begun. The 
      increased transcriptional complexity of mammalian systems provides a wider array 
      of targets for RNA surveillance, and, while many questions remain unanswered, 
      emerging data suggest the nuclear RNA surveillance machinery exhibits increased 
      complexity as well. We have used a small interfering RNA in mouse N2A cells to 
      target the homolog of a yeast protein that functions in RNA surveillance (Mtr4p). 
      We used high-throughput sequencing of polyadenylated RNAs (PA-seq) to quantify 
      the effects of the mMtr4 knockdown (KD) on RNA surveillance. We demonstrate that 
      overall abundance of polyadenylated protein coding mRNAs is not affected, but 
      several targets of RNA surveillance predicted from work in yeast accumulate as 
      adenylated RNAs in the mMtr4KD. microRNAs are an added layer of transcriptional 
      complexity not found in yeast. After Drosha cleavage separates the pre-miRNA from 
      the microRNA's primary transcript, the byproducts of that transcript are 
      generally thought to be degraded. We have identified the 5' leading segments of 
      pri-miRNAs as novel targets of mMtr4 dependent RNA surveillance.
FAU - Dorweiler, Jane E
AU  - Dorweiler JE
AD  - Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin, 
      United States of America.
FAU - Ni, Ting
AU  - Ni T
AD  - DNA Sequencing and Genomics Core, Genetics and Development Biology Center, 
      National Institutes of Health, National Heart Lung and Blood Institute, Bethesda, 
      Maryland, United States of America.
FAU - Zhu, Jun
AU  - Zhu J
AD  - DNA Sequencing and Genomics Core, Genetics and Development Biology Center, 
      National Institutes of Health, National Heart Lung and Blood Institute, Bethesda, 
      Maryland, United States of America.
FAU - Munroe, Stephen H
AU  - Munroe SH
AD  - Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin, 
      United States of America.
FAU - Anderson, James T
AU  - Anderson JT
AD  - Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin, 
      United States of America.
LA  - eng
GR  - R15 GM100445/GM/NIGMS NIH HHS/United States
GR  - ImNIH/Intramural NIH HHS/United States
GR  - 1R15GM100445-01A1/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140613
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (5' Untranslated Regions)
RN  - 0 (MicroRNAs)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA-Binding Proteins)
RN  - 24937-83-5 (Poly A)
RN  - EC 3.1.26.3 (Drosha protein, mouse)
RN  - EC 3.1.26.3 (Ribonuclease III)
RN  - EC 3.6.4.13 (RNA Helicases)
RN  - EC 3.6.4.13 (SKIV2L2 protein, mouse)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Animals
MH  - Cell Line
MH  - Gene Knockdown Techniques
MH  - High-Throughput Nucleotide Sequencing
MH  - Mice
MH  - MicroRNAs/*genetics/*metabolism
MH  - Nuclear Proteins/*genetics/*metabolism
MH  - Poly A/metabolism
MH  - RNA Helicases
MH  - RNA Stability
MH  - RNA, Small Interfering/metabolism
MH  - RNA-Binding Proteins/*genetics/*metabolism
MH  - Ribonuclease III/metabolism
MH  - Sequence Analysis, RNA
PMC - PMC4057207
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/06/14 06:00
MHDA- 2015/10/27 06:00
PMCR- 2014/06/13
CRDT- 2014/06/14 06:00
PHST- 2014/03/27 00:00 [received]
PHST- 2014/05/14 00:00 [accepted]
PHST- 2014/06/14 06:00 [entrez]
PHST- 2014/06/14 06:00 [pubmed]
PHST- 2015/10/27 06:00 [medline]
PHST- 2014/06/13 00:00 [pmc-release]
AID - PONE-D-14-13808 [pii]
AID - 10.1371/journal.pone.0099430 [doi]
PST - epublish
SO  - PLoS One. 2014 Jun 13;9(6):e99430. doi: 10.1371/journal.pone.0099430. eCollection 
      2014.