PMID- 24926808
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20151123
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 62
IP  - 28
DP  - 2014 Jul 16
TI  - Decreased immunoglobulin E (IgE) binding to cashew allergens following sodium 
      sulfite treatment and heating.
PG  - 6746-55
LID - 10.1021/jf501117p [doi]
AB  - Cashew nut and other nut allergies can result in serious and sometimes 
      life-threatening reactions. Linear and conformational epitopes within food 
      allergens are important for immunoglobulin E (IgE) binding. Methods that disrupt 
      allergen structure can lower IgE binding and lessen the likelihood of food 
      allergy reactions. Previous structural and biochemical data have indicated that 
      2S albumins from tree nuts and peanuts are potent allergens, and that their 
      structures are sensitive to strong reducing agents such as dithiothreitol. This 
      study demonstrates that the generally regarded as safe (GRAS) compound sodium 
      sulfite effectively disrupted the structure of the cashew 2S albumin, Ana o 3, in 
      a temperature-dependent manner. This study also showed that sulfite is effective 
      at disrupting the disulfide bond within the cashew legumin, Ana o 2. 
      Immunoblotting and ELISA demonstrated that the binding of cashew proteins by 
      rabbit IgG or IgE from cashew-allergic patients was markedly lowered following 
      treatment with sodium sulfite and heating. The results indicate that 
      incorporation of sodium sulfite, or other food grade reagents with similar redox 
      potential, may be useful processing methods to lower or eliminate IgE binding to 
      food allergens.
FAU - Mattison, Christopher P
AU  - Mattison CP
AD  - Southern Regional Research Center, Agricultural Research Service, U.S. Department 
      of Agriculture, New Orleans, Louisiana 70124, United States.
FAU - Desormeaux, Wendy A
AU  - Desormeaux WA
FAU - Wasserman, Richard L
AU  - Wasserman RL
FAU - Yoshioka-Tarver, Megumi
AU  - Yoshioka-Tarver M
FAU - Condon, Brian
AU  - Condon B
FAU - Grimm, Casey C
AU  - Grimm CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140701
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (2S Albumins, Plant)
RN  - 0 (Allergens)
RN  - 0 (Ana o 2 allergen, cashew)
RN  - 0 (Ana o 3 protein, Anacardium occidentale)
RN  - 0 (Antigens, Plant)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Plant Proteins)
RN  - 0 (Sulfites)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - VTK01UQK3G (sodium sulfite)
SB  - IM
MH  - 2S Albumins, Plant/chemistry/immunology
MH  - Adolescent
MH  - Adult
MH  - Allergens/chemistry/drug effects/*immunology
MH  - Amino Acid Sequence
MH  - Anacardium/*immunology
MH  - Animals
MH  - Antigens, Plant/chemistry/drug effects/immunology
MH  - Child
MH  - Food Hypersensitivity/immunology
MH  - *Hot Temperature
MH  - Humans
MH  - Immunoglobulin E/*metabolism
MH  - Immunoglobulin G/metabolism
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Molecular Structure
MH  - Nuts/immunology
MH  - Plant Proteins/chemistry/drug effects/immunology
MH  - Rabbits
MH  - Sulfites/*pharmacology
EDAT- 2014/06/14 06:00
MHDA- 2015/04/10 06:00
CRDT- 2014/06/14 06:00
PHST- 2014/06/14 06:00 [entrez]
PHST- 2014/06/14 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
AID - 10.1021/jf501117p [doi]
PST - ppublish
SO  - J Agric Food Chem. 2014 Jul 16;62(28):6746-55. doi: 10.1021/jf501117p. Epub 2014 
      Jul 1.