PMID- 24927441
OWN - NLM
STAT- MEDLINE
DCOM- 20141016
LR  - 20141120
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 16
IP  - 6
DP  - 2014 Jun
TI  - [A preliminary study of plasma microRNA levels in children with methylmalonic 
      acidemia].
PG  - 629-33
AB  - OBJECTIVE: To screen out differentially expressed microRNAs (miRNAs) in the 
      plasma of children with methylmalonic acidemia (MMA), to determine the expression 
      of miR-9-1 in plasma and to preliminarily evaluate the significance of miR-9-1 as 
      a biomarker in MMA. METHODS: Plasma was obtained from 17 MMA children, 10 
      hyperhomocysteinemia (HHcy) children without MMA (HHcy group), and 10 normal 
      controls. Of 17 MMA children, 12 had HHcy (MMA+HHcy group), and 5 had no HHcy 
      (MMA group). The differentially expressed miRNAs were screened out by miRNA 
      microarray. Differentially expressed miR-9-1 was selected, and plasma miR-9-1 
      levels were determined by RT-PCR. Urine was collected from MMA patients who 
      received vitamin B12 treatment, and plasma miR-9-1 levels were determined by 
      RT-PCR after treatment. RESULTS: The miRNA microarray analysis showed that 26 
      miRNAs were differentially expressed, among which 16 miRNAs (including miR-9-1) 
      were down-regulated over 2 times, while 10 miRNAs were up-regulated over 2 times. 
      The MMA+HHcy , MMA and HHcy groups had significantly down-regulated miR-9-1 
      compared with the normal control group (P<0.01). The patients who showed a good 
      response to vitamin B12 treatment had significantly increased plasma miR-9-1 
      levels, without significant difference compared with the normal control group. 
      CONCLUSIONS: Plasma miR-9-1 is significantly down-regulated in MMA patients, but 
      it is significantly up-regulated after vitamin B12 treatment, suggesting that 
      miR-9-1 may act as a biomarker in monitoring the progression of MMA.
FAU - Li, Yan-Fei
AU  - Li YF
AD  - Department of Neurology, First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, China. jiayanjie1971@yahoo.com.cn.
FAU - Peng, Tao
AU  - Peng T
FAU - Duan, Ran-Ran
AU  - Duan RR
FAU - Wang, Xiao-Han
AU  - Wang XH
FAU - Gao, Hui-Li
AU  - Gao HL
FAU - Wang, Jing-Tao
AU  - Wang JT
FAU - Teng, Jun-Fang
AU  - Teng JF
FAU - Jia, Yan-Jie
AU  - Jia YJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - 0 (MIRN92 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - Homocysteinemia
RN  - Methylmalonic acidemia
SB  - IM
MH  - Adolescent
MH  - Amino Acid Metabolism, Inborn Errors/*genetics
MH  - Child
MH  - Female
MH  - Humans
MH  - Hyperhomocysteinemia/genetics
MH  - Male
MH  - MicroRNAs/*blood
EDAT- 2014/06/14 06:00
MHDA- 2014/10/17 06:00
CRDT- 2014/06/14 06:00
PHST- 2014/06/14 06:00 [entrez]
PHST- 2014/06/14 06:00 [pubmed]
PHST- 2014/10/17 06:00 [medline]
AID - 10.7499/j.issn.1008-8830.2014.06.014 [pii]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2014 Jun;16(6):629-33.