PMID- 24927715
OWN - NLM
STAT- MEDLINE
DCOM- 20151207
LR  - 20181202
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 16
IP  - 10
DP  - 2014 Oct
TI  - Mechanism of mesenchymal stem cell-induced neuron recovery and anti-inflammation.
PG  - 1336-44
LID - S1465-3249(14)00605-7 [pii]
LID - 10.1016/j.jcyt.2014.05.007 [doi]
AB  - BACKGROUND AIMS: After ischemic or hemorrhagic stroke, neurons in the penumbra 
      surrounding regions of irreversible injury are vulnerable to delayed but 
      progressive damage as a result of ischemia and hemin-induced neurotoxicity. There 
      is no effective treatment to rescue such dying neurons. Mesenchymal stem cells 
      (MSCs) hold promise for rescue of these damaged neurons. In this study, we 
      evaluated the efficacy and mechanism of MSC-induced neuro-regeneration and immune 
      modulation. METHODS: Oxygen-glucose deprivation (OGD) was used in our study. M17 
      neuronal cells were subjected to OGD stress then followed by co-culture with 
      MSCs. Rescue effects were evaluated using proliferation and apoptosis assays. 
      Cytokine assay and quantitative polymerase chain reaction were used to explore 
      the underlying mechanism. Antibody and small molecule blocking experiments were 
      also performed to further understand the mechanism. RESULTS: We showed that M17 
      proliferation was significantly decreased and the rate of apoptosis increased 
      after exposure to OGD. These effects could be alleviated via co-culture with 
      MSCs. Tumor necrosis factor-alpha was found elevated after OGD stress and was back to 
      normal levels after co-culture with MSCs. We believe these effects involve 
      interleukin-6 and vascular endothelial growth factor signaling pathways. 
      DISCUSSION: Our studies have shown that MSCs have anti-inflammatory properties 
      and the capacity to rescue injured neurons.
CI  - Copyright (c) 2014 International Society for Cellular Therapy. Published by 
      Elsevier Inc. All rights reserved.
FAU - Huang, Peng
AU  - Huang P
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, 
      Florida, USA.
FAU - Gebhart, Nichole
AU  - Gebhart N
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, 
      Florida, USA.
FAU - Richelson, Elliott
AU  - Richelson E
AD  - Department of Pharmacology and Psychiatry, Mayo Clinic, Jacksonville, Florida, 
      USA.
FAU - Brott, Thomas G
AU  - Brott TG
AD  - Department of Neurology, Mayo Clinic, Jacksonville, Florida, USA.
FAU - Meschia, James F
AU  - Meschia JF
AD  - Department of Neurology, Mayo Clinic, Jacksonville, Florida, USA.
FAU - Zubair, Abba C
AU  - Zubair AC
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, 
      Florida, USA. Electronic address: Zubair.Abba@Mayo.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140611
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (TNF protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Apoptosis/physiology
MH  - *Cell Communication
MH  - Cell Hypoxia/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Glucose/deficiency/metabolism
MH  - Humans
MH  - Inflammation/metabolism/pathology/*prevention & control
MH  - Interleukin-6/metabolism/pharmacology
MH  - Mesenchymal Stem Cells/metabolism/*physiology
MH  - Neuritis/metabolism/*prevention & control
MH  - Neurons/drug effects/pathology/*physiology
MH  - Stroke/pathology/*therapy
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism/pharmacology
OTO - NOTNLM
OT  - MSC
OT  - immune modulation
OT  - neuro-regeneration
OT  - stroke
EDAT- 2014/06/15 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/06/15 06:00
PHST- 2014/01/10 00:00 [received]
PHST- 2014/04/05 00:00 [revised]
PHST- 2014/05/02 00:00 [accepted]
PHST- 2014/06/15 06:00 [entrez]
PHST- 2014/06/15 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S1465-3249(14)00605-7 [pii]
AID - 10.1016/j.jcyt.2014.05.007 [doi]
PST - ppublish
SO  - Cytotherapy. 2014 Oct;16(10):1336-44. doi: 10.1016/j.jcyt.2014.05.007. Epub 2014 
      Jun 11.