PMID- 24927918
OWN - NLM
STAT- MEDLINE
DCOM- 20150611
LR  - 20211021
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 358
IP  - 1
DP  - 2014 Oct
TI  - Characterization and evaluation of mesenchymal stem cells derived from human 
      embryonic stem cells and bone marrow.
PG  - 149-64
LID - 10.1007/s00441-014-1926-5 [doi]
AB  - Embryonic stem cells (ESCs) and mesenchymal stem cells (MSCs) have been studied 
      for years as primary cell sources for regenerative biology and medicine. MSCs 
      have been derived from cell and tissue sources, such as bone marrow (BM), and 
      more recently from ESCs. This study investigated MSCs derived from BM, H1- and 
      H9-ESC lines in terms of morphology, surface marker and growth factor receptor 
      expression, proliferative capability, modulation of immune cell growth and 
      multipotency, in order to evaluate ESC-MSCs as a cell source for potential 
      regenerative applications. The results showed that ESC-MSCs exhibited 
      spindle-shaped morphology similar to BM-MSCs but of various sizes, and flow 
      cytometric immunophenotyping revealed expression of characteristic MSC surface 
      markers on all tested cell lines except H9-derived MSCs. Differences in growth 
      factor receptor expression were also shown between cell lines. In addition, 
      ESC-MSCs showed greater capabilities for cell proliferation, and suppression of 
      leukocyte growth compared to BM-MSCs. Using standard protocols, induction of 
      ESC-MSC differentiation along the adipogenic, osteogenic, or chondrogenic 
      lineages was less effective compared to that of BM-MSCs. By adding bone 
      morphogenetic protein 7 (BMP7) into transforming growth factor beta 1 
      (TGFbeta1)-supplemented induction medium, chondrogenesis of ESC-MSCs was 
      significantly enhanced. Our findings suggest that ESC-MSCs and BM-MSCs show 
      differences in their surface marker profiles and the capacities of proliferation, 
      immunomodulation, and most importantly multi-lineage differentiation. Using 
      modified chondrogenic medium with BMP7 and TGFbeta1, H1-MSCs can be effectively 
      induced as BM-MSCs for chondrogenesis.
FAU - Brown, Patrick T
AU  - Brown PT
AD  - Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, 
      1685 Highland Avenue, UWMFCB Room 6227, Madison, WI, 53705, USA, 
      ptbrown2@wisc.edu.
FAU - Squire, Matthew W
AU  - Squire MW
FAU - Li, Wan-Ju
AU  - Li WJ
LA  - eng
GR  - R25 GM083252/GM/NIGMS NIH HHS/United States
GR  - T32 GM008692/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140614
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (BMP7 protein, human)
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - Bone Morphogenetic Protein 7/pharmacology
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cell Line
MH  - Cell Proliferation/drug effects/*physiology
MH  - Cell Shape/drug effects/physiology
MH  - Embryonic Stem Cells/cytology/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Transforming Growth Factor beta1/pharmacology
PMC - PMC4329984
MID - NIHMS661013
COIS- Disclosure statement The authors have no financial conflicts to disclose.
EDAT- 2014/06/15 06:00
MHDA- 2015/06/13 06:00
PMCR- 2015/02/16
CRDT- 2014/06/15 06:00
PHST- 2013/07/19 00:00 [received]
PHST- 2014/05/15 00:00 [accepted]
PHST- 2014/06/15 06:00 [entrez]
PHST- 2014/06/15 06:00 [pubmed]
PHST- 2015/06/13 06:00 [medline]
PHST- 2015/02/16 00:00 [pmc-release]
AID - 10.1007/s00441-014-1926-5 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2014 Oct;358(1):149-64. doi: 10.1007/s00441-014-1926-5. Epub 
      2014 Jun 14.