PMID- 24928046
OWN - NLM
STAT- MEDLINE
DCOM- 20140902
LR  - 20140614
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 458-459
DP  - 2014 Jun
TI  - Biogenesis of non-structural protein 1 (nsp1) and nsp1-mediated type I interferon 
      modulation in arteriviruses.
PG  - 136-50
LID - S0042-6822(14)00151-2 [pii]
LID - 10.1016/j.virol.2014.04.028 [doi]
AB  - Type I interferons (IFNs-alpha/beta) play a key role for the antiviral state of host, 
      and the porcine arterivirus; porcine reproductive and respiratory syndrome virus 
      (PRRSV), has been shown to down-regulate the production of IFNs during infection. 
      Non-structural protein (nsp) 1 of PRRSV has been identified as a viral IFN 
      antagonist, and the nsp1alpha subunit of nsp1 has been shown to degrade the 
      CREB-binding protein (CBP) and to inhibit the formation of enhanceosome thus 
      resulting in the suppression of IFN production. The study was expanded to other 
      member viruses in the family Arteriviridae: equine arteritis virus (EAV), murine 
      lactate dehydrogenase-elevating virus (LDV), and simian hemorrhagic fever virus 
      (SHFV). While PRRSV-nsp1 and LDV-nsp1 were auto-cleaved to produce the nsp1alpha and 
      nsp1beta subunits, EAV-nsp1 remained uncleaved. SHFV-nsp1 was initially predicted to 
      be cleaved to generate three subunits (nsp1alpha, nsp1beta, and nsp1gamma), but only two 
      subunits were generated as SHFV-nsp1alphabeta and SHFV-nsp1gamma. The papain-like cysteine 
      protease (PLP) 1alpha motif in nsp1alpha remained inactive for SHFV, and only the PLP1beta 
      motif of nsp1beta was functional to generate SHFV-nsp1gamma subunit. All subunits of 
      arterivirus nsp1 were localized in the both nucleus and cytoplasm, but 
      PRRSV-nsp1beta, LDV-nsp1beta, EAV-nsp1, and SHFV-nsp1gamma were predominantly found in the 
      nucleus. All subunits of arterivirus nsp1 contained the IFN suppressive activity 
      and inhibited both interferon regulatory factor 3 (IRF3) and NF-kappaB mediated IFN 
      promoter activities. Similar to PRRSV-nsp1alpha, CBP degradation was evident in cells 
      expressing LDV-nsp1alpha and SHFV-nsp1gamma, but no such degradation was observed for 
      EAV-nsp1. Regardless of CBP degradation, all subunits of arterivirus nsp1 
      suppressed the IFN-sensitive response element (ISRE)-promoter activities. Our 
      data show that the nsp1-mediated IFN modulation is a common strategy for all 
      arteriviruses but their mechanism of action may differ from each other.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Han, Mingyuan
AU  - Han M
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA.
FAU - Kim, Chi Yong
AU  - Kim CY
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA.
FAU - Rowland, Raymond R R
AU  - Rowland RR
AD  - Department of Diagnostic Medicine and Pathobiology, Kansas State University, 
      Manhattan, KS 66506, USA.
FAU - Fang, Ying
AU  - Fang Y
AD  - Department of Diagnostic Medicine and Pathobiology, Kansas State University, 
      Manhattan, KS 66506, USA.
FAU - Kim, Daewoo
AU  - Kim D
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA.
FAU - Yoo, Dongwan
AU  - Yoo D
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA. Electronic address: 
      dyoo@illinois.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140513
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Interferon Type I)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Arteriviridae/genetics/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Gene Expression Regulation, Viral/*physiology
MH  - Humans
MH  - Interferon Type I/*metabolism
MH  - Viral Nonstructural Proteins/genetics/*metabolism
MH  - Virus Replication
OTO - NOTNLM
OT  - Arteriviridae
OT  - CBP degradation
OT  - EAV
OT  - IFN antagonism
OT  - LDV
OT  - PRRSV
OT  - SHFV
OT  - nsp1
EDAT- 2014/06/15 06:00
MHDA- 2014/09/03 06:00
CRDT- 2014/06/15 06:00
PHST- 2014/02/07 00:00 [received]
PHST- 2014/03/03 00:00 [revised]
PHST- 2014/04/22 00:00 [accepted]
PHST- 2014/06/15 06:00 [entrez]
PHST- 2014/06/15 06:00 [pubmed]
PHST- 2014/09/03 06:00 [medline]
AID - S0042-6822(14)00151-2 [pii]
AID - 10.1016/j.virol.2014.04.028 [doi]
PST - ppublish
SO  - Virology. 2014 Jun;458-459:136-50. doi: 10.1016/j.virol.2014.04.028. Epub 2014 
      May 13.