PMID- 24929145
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20140804
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 75
IP  - 8
DP  - 2014 Aug
TI  - HLA-G gene expression influenced at allelic level in association with end stage 
      renal disease and acute allograft rejection.
PG  - 833-9
LID - S0198-8859(14)00169-4 [pii]
LID - 10.1016/j.humimm.2014.06.005 [doi]
AB  - BACKGROUND: Human leukocyte antigen (HLA)-G is a non-classical 
      major-histocompatibility complex class-I molecule associated with 
      immunosuppressive function. We have evaluated the impact of HLA-G allele 
      associated with untranslated-region (UTR)-haplotype in end stage renal disease 
      (ESRD) and acute allograft rejection (AR) cases. The mRNA levels of different 
      HLA-G isoforms were evaluated in ESRD and AR cases. Subsequently, the total HLA-G 
      mRNA levels and protein concentration were evaluated against its UTR-haplotype 
      among ESRD and AR cases. METHODOLOGY: Sequence based typing of the promoter 
      region was carried-out to evaluate the impact of HLA-G haplotype in 350 ESRD 
      cases and 300 controls. HLA-G gene expression was evaluated at the 
      transcriptional level using semi-quantitative and quantitative PCR, whereas 
      protein concentration was determined by ELISA among both cases and control. 
      RESULTS: Increased risk was observed for G*01:01:01:03, G*01:01:02, G*01:06 and 
      G*01:05:N haplotypes while G*01:01:01:01 and G*01:04:01 haplotypes showed a 
      protective effect in ESRD and AR cases. Higher level of soluble HLA-G isoforms 
      (G5 and G6) was observed among ESRD cases. Reduced levels of soluble isoform (G5) 
      and increased levels of membrane bound (G1 and G3) isoforms were found among AR 
      cases, revealing risk association. Decreased HLA-G expression was observed at 
      both mRNA and protein level for G*01:01:01:03 and G*01:05:N haplotypes in ESRD 
      and AR cases. CONCLUSIONS: These results suggest that the variation in the 
      expression profile of membrane bound and soluble isoforms may modulate the risk 
      for ESRD and AR. UTR-haplotypes appear to be involved in different HLA-G 
      expression patterns at transcriptional and translational levels.
CI  - Copyright (c) 2014 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Misra, Maneesh Kumar
AU  - Misra MK
AD  - Department of Medical Genetics, Sanjay Gandhi Post-graduate Institute of Medical 
      Sciences, Raebareli Road, Lucknow 226014, UP, India; Department of Anatomy, 
      Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, UP, 
      India.
FAU - Pandey, Shashi Kant
AU  - Pandey SK
AD  - Department of Anatomy, Institute of Medical Sciences, Banaras Hindu University, 
      Varanasi 221005, UP, India.
FAU - Kapoor, Rakesh
AU  - Kapoor R
AD  - Department of Urology and Renal Transplantation, SGPGIMS, Lucknow 226014, UP, 
      India.
FAU - Sharma, Raj Kumar
AU  - Sharma RK
AD  - Department of Nephrology, SGPGIMS, Lucknow 226014, UP, India.
FAU - Kapoor, Rohit
AU  - Kapoor R
AD  - Department of Surgery, Era's Lucknow Medical College and Hospital, Lucknow 
      226003, India.
FAU - Prakash, Swayam
AU  - Prakash S
AD  - Department of Medical Genetics, Sanjay Gandhi Post-graduate Institute of Medical 
      Sciences, Raebareli Road, Lucknow 226014, UP, India.
FAU - Agrawal, Suraksha
AU  - Agrawal S
AD  - Department of Medical Genetics, Sanjay Gandhi Post-graduate Institute of Medical 
      Sciences, Raebareli Road, Lucknow 226014, UP, India. Electronic address: 
      sur_ksha_agrawal@yahoo.co.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140611
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-G Antigens)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Alleles
MH  - Case-Control Studies
MH  - Female
MH  - Gene Expression
MH  - Gene Frequency
MH  - Graft Rejection/*genetics/immunology/pathology
MH  - HLA-G Antigens/*genetics/immunology
MH  - Haplotypes
MH  - Humans
MH  - Kidney Failure, Chronic/*genetics/immunology/pathology/surgery
MH  - *Kidney Transplantation
MH  - Male
MH  - Middle Aged
MH  - *Promoter Regions, Genetic
MH  - Protein Isoforms/genetics/immunology
MH  - Risk
MH  - Solubility
MH  - Transplantation, Homologous
OTO - NOTNLM
OT  - Acute allograft rejection
OT  - End stage renal disease
OT  - HLA-G allele
OT  - HLA-G expression
OT  - UTR haplotype
EDAT- 2014/06/15 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/06/15 06:00
PHST- 2013/11/12 00:00 [received]
PHST- 2014/06/03 00:00 [revised]
PHST- 2014/06/03 00:00 [accepted]
PHST- 2014/06/15 06:00 [entrez]
PHST- 2014/06/15 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - S0198-8859(14)00169-4 [pii]
AID - 10.1016/j.humimm.2014.06.005 [doi]
PST - ppublish
SO  - Hum Immunol. 2014 Aug;75(8):833-9. doi: 10.1016/j.humimm.2014.06.005. Epub 2014 
      Jun 11.