PMID- 24929352
OWN - NLM
STAT- MEDLINE
DCOM- 20140923
LR  - 20181202
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 154
DP  - 2014 Sep
TI  - Effects of water accommodated fractions of crude oils and diesel on a suite of 
      biomarkers in Atlantic cod (Gadus morhua).
PG  - 240-52
LID - S0166-445X(14)00172-6 [pii]
LID - 10.1016/j.aquatox.2014.05.013 [doi]
AB  - The aim of this study was to characterize concentration- and time-dependent 
      responses in juvenile Atlantic cod (Gadus morhua) following exposure for one and 
      three weeks to the water-soluble fraction (WAF) of three weathered oils: Arabian 
      Light crude oil (ALC), North Sea crude oil (NSC) and ship-diesel. The sum of 
      polycyclic aromatic hydrocarbons (PAH) in water was highest after one week of 
      exposure and within environmentally relevant concentrations. PAH metabolites in 
      bile confirmed exposure to and uptake of PAHs. Hepatic cytochrome P450 1A (CYP1A) 
      gene expression (mRNA quantification) increased dramatically following exposure 
      to all three oil types (fold-change up to 165) and there was a time lag between 
      gene and protein expression. Hepatic CYP1A protein concentration and 
      ethoxyresorufin-O-deethylase (EROD) activity were more variable among individuals 
      and treatments than gene expression. EROD activity in liver and gills increased 
      in fish exposed to WAF from the two crude oils, but not in fish exposed to WAF 
      from diesel. Exposure to diesel appeared to induce oxidative stress to a greater 
      extent than exposure to crude oils. Other biomarkers (glutathione S-transferases, 
      acetylcholine esterase, vitellogenin) did not appear to respond to the exposure 
      and hence did not discriminate among oils. Biomarker responses in cod after 
      exposure to weathered crude oils and diesel suggested that the CYP1A system and 
      oxidative stress markers have the highest potential for discriminating among 
      different oil types and to monitor the environmental consequences of spills.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Holth, T F
AU  - Holth TF
AD  - Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, N-0316 
      Oslo, Norway. Electronic address: t.f.holth@ibv.uio.no.
FAU - Eidsvoll, D P
AU  - Eidsvoll DP
AD  - Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, N-0316 
      Oslo, Norway.
FAU - Farmen, E
AU  - Farmen E
AD  - Norwegian Institute for Water Research (NIVA), Gaustadaleen 21, N-0349 Oslo, 
      Norway.
FAU - Sanders, M B
AU  - Sanders MB
AD  - CEFAS Weymouth Laboratory, The Nothe, Barrack Road, Weymouth, Dorset DT4 8UB, 
      England, United Kingdom.
FAU - Martinez-Gomez, C
AU  - Martinez-Gomez C
AD  - Instituto Espanol de Oceanografia, San Pedro del Pinatar, 30740 Varadero 1, 
      Spain.
FAU - Budzinski, H
AU  - Budzinski H
AD  - University of Bordeaux, EPOC/LPTC (UMR 5805 CNRS), 351 crs de la Liberation, 
      Talence, France.
FAU - Burgeot, T
AU  - Burgeot T
AD  - IFREMER, Unit of Research in Biogeochemistry and Ecotoxicology, Rue de I'lle 
      d'Yeu, BP 21105, 44311 Nantes, France.
FAU - Guilhermino, L
AU  - Guilhermino L
AD  - CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, 
      Laboratory of Ecotoxicology and Ecology & ICBAS - Institute of Biomedical 
      Sciences of Abel Salazar, Department of Population Studies, Laboratory of 
      Ecotoxicology, University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal.
FAU - Hylland, K
AU  - Hylland K
AD  - Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, N-0316 
      Oslo, Norway.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140516
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Biomarkers)
RN  - 0 (Gasoline)
RN  - 0 (Petroleum)
RN  - 0 (Water Pollutants, Chemical)
RN  - 059QF0KO0R (Water)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Bile/chemistry
MH  - Biomarkers/*analysis
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - Gadus morhua/*physiology
MH  - Gasoline/*toxicity
MH  - Gills/chemistry
MH  - Humans
MH  - Liver/chemistry/drug effects
MH  - North Sea
MH  - Oxidative Stress/*drug effects
MH  - Petroleum/analysis/*toxicity
MH  - Water/*chemistry
MH  - Water Pollutants, Chemical/analysis/toxicity
OTO - NOTNLM
OT  - Atlantic cod
OT  - Biomarkers
OT  - Ship-diesel
OT  - Time-course
OT  - Water accommodated fraction
OT  - Weathered crude oil
EDAT- 2014/06/16 06:00
MHDA- 2014/09/24 06:00
CRDT- 2014/06/16 06:00
PHST- 2013/12/30 00:00 [received]
PHST- 2014/05/05 00:00 [revised]
PHST- 2014/05/10 00:00 [accepted]
PHST- 2014/06/16 06:00 [entrez]
PHST- 2014/06/16 06:00 [pubmed]
PHST- 2014/09/24 06:00 [medline]
AID - S0166-445X(14)00172-6 [pii]
AID - 10.1016/j.aquatox.2014.05.013 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2014 Sep;154:240-52. doi: 10.1016/j.aquatox.2014.05.013. Epub 2014 
      May 16.