PMID- 24929514
OWN - NLM
STAT- MEDLINE
DCOM- 20140821
LR  - 20161020
IS  - 0253-9772 (Print)
IS  - 0253-9772 (Linking)
VI  - 36
IP  - 6
DP  - 2014 Jun
TI  - [Identification of chromosomal aberration in esophageal cancer cells by mixed BAC 
      DNA probes of chromosome arms and regions].
PG  - 558-65
LID - 10.3724/SP.J.1005.2014.0558 [doi]
AB  - Chromosomal aberration is an important genetic feature of malignant tumor cells. 
      This study aimed to clarify whether BAC DNA could be used to identify chromosome 
      region and arm alterations. For each chromosome region, five to ten 1 Mb BAC DNA 
      clones were selected to construct mixed BAC DNA clones for the particular region. 
      All of the mixed clones from regions which could cover the whole chromosome arm 
      were then mixed to construct mixed BAC DNA clones for the arms. Mixed BAC DNA 
      probes of arms and regions were labeled by degenerate oligonucleotide primed PCR 
      (DOP-PCR) and Nick translation techniques, respectively. The specificities of 
      these probes were validated by fluorescence in situ hybridization (FISH) on the 
      metaphase chromosomes of normal human peripheral blood lymphocytes. FISH with 
      arm-specific mixed BAC DNA probes showed that chromosomal rearrangements and 
      involved chromosome arms were confirmed in several esophageal cancer cells. By 
      using region-specific mixed probes, the breakpoint on 1q from the derivative 
      chromosome t(1q;7q) was identified in 1q32-q41 in esophageal KYSE140 cells. In 
      conclusion, we established an effective labeling method for 1 Mb BAC DNA mixed 
      clone probes, and chromosome arm and region rearrangements could be identified in 
      several esophageal cancer cells by using these probes. Our study provides a more 
      precise method for identification of chromosomal aberration by M-FISH, and the 
      established method may also be applied to the karyotype analysis of hematological 
      malignancies and prenatal diagnosis.
FAU - Jiajie, Hao
AU  - Jiajie H
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, 
      China.
FAU - Chunli, Wang
AU  - Chunli W
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, 
      China; Department of Histology and Embryology, Anhui Medical University, Hefei 
      230032, China;
FAU - Wenyue, Gu
AU  - Wenyue G
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, 
      China; Department of Pathology, Yancheng Third People's Hospital, Yancheng 
      224001, China;
FAU - Xiaoyu, Cheng
AU  - Xiaoyu C
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, 
      China.
FAU - Yu, Zhang
AU  - Yu Z
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, 
      China.
FAU - Xin, Xu
AU  - Xin X
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, 
      China.
FAU - Yan, Cai
AU  - Yan C
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, 
      China.
FAU - Mingrong, Wang
AU  - Mingrong W
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, 
      China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan
JT  - Yi chuan = Hereditas
JID - 9436478
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Chromosomes, Human/*genetics
MH  - DNA Probes/*genetics
MH  - Esophageal Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
EDAT- 2014/06/16 06:00
MHDA- 2014/08/22 06:00
CRDT- 2014/06/16 06:00
PHST- 2014/06/16 06:00 [entrez]
PHST- 2014/06/16 06:00 [pubmed]
PHST- 2014/08/22 06:00 [medline]
AID - 10.3724/SP.J.1005.2014.0558 [doi]
PST - ppublish
SO  - Yi Chuan. 2014 Jun;36(6):558-65. doi: 10.3724/SP.J.1005.2014.0558.