PMID- 24929550
OWN - NLM
STAT- MEDLINE
DCOM- 20150212
LR  - 20201209
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 963
DP  - 2014 Jul 15
TI  - Simultaneous quantitative determination of microcystin-LR and its glutathione 
      metabolites in rat liver by liquid chromatography-tandem mass spectrometry.
PG  - 54-61
LID - S1570-0232(14)00361-4 [pii]
LID - 10.1016/j.jchromb.2014.05.046 [doi]
AB  - The roles of glutathione (GSH) and cysteine (Cys) in the detoxification of 
      Microcystin-LR (MC-LR) have recently become a popular area of research. However, 
      lacking analysis methods for MC-LR-GSH and MC-LR-Cys (two main GSH pathway 
      metabolites) in mammals, elucidation of the detoxification mechanism and 
      metabolic pathway of MC-LR in mammals is difficult. In this study, a novel method 
      for the simultaneous quantitative analysis of MC-LR, MC-LR-GSH and MC-LR-Cys in 
      rat liver was developed and validated. The analytes were simultaneously extracted 
      from rat liver using 3M sodium chloride solution containing 0.01M EDTA-Na2-5% 
      acetic acid, followed by solid-phase extraction (SPE) on Oasis HLB and silica 
      cartridges and determination by liquid chromatography-electrospray ionization 
      mass spectrometry (LC-ESI-MS/MS). Under the optimized pretreatment conditions and 
      instrument parameters, good recoveries of MC-LR, MC-LR-GSH and MC-LR-Cys were 
      obtained at three concentrations (0.2, 1.0 and 2.5 mug g(-1) dry weight (DW)) with 
      values ranging from 97.7 +/- 4.2 to 98.7 +/- 5.1%, 70.1 +/- 4.8 to 71.1 +/- 4.1% and 79.8 
      +/- 3.5 to 81.4 +/- 4.0%, respectively. The relative standard deviations (RSDs) of 
      these compounds at 0.2, 1.0 and 2.5 mug g(-1) DW were between 4.3% and 6.9%. The 
      limits of detection (LODs) were 0.005, 0.007 and 0.006 mug g(-1) DW and the limits 
      of quantification (LOQs) were 0.017, 0.023 and 0.020 mug g(-1) DW for MC-LR, 
      MC-LR-GSH and MC-LR-Cys, respectively. Furthermore, this method was successfully 
      applied to both time- and dosage-effect studies of MC-LR, MC-LR-GSH and MC-LR-Cys 
      in vivo.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Guo, Xiaochun
AU  - Guo X
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, 
      China. Electronic address: guoxiaochun419@163.com.
FAU - Xie, Ping
AU  - Xie P
AD  - Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of 
      Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese 
      Academy of Sciences, Donghu South Road 7, Wuhan 430072, China. Electronic 
      address: xieping@ihb.ac.cn.
FAU - Chen, Jun
AU  - Chen J
AD  - Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of 
      Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese 
      Academy of Sciences, Donghu South Road 7, Wuhan 430072, China. Electronic 
      address: chenjun@ihb.ac.cn.
FAU - Tuo, Xun
AU  - Tuo X
AD  - Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of 
      Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese 
      Academy of Sciences, Donghu South Road 7, Wuhan 430072, China.
FAU - Deng, Xuwei
AU  - Deng X
AD  - Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of 
      Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese 
      Academy of Sciences, Donghu South Road 7, Wuhan 430072, China.
FAU - Li, Shangchun
AU  - Li S
AD  - Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of 
      Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese 
      Academy of Sciences, Donghu South Road 7, Wuhan 430072, China.
FAU - Yu, Dezhao
AU  - Yu D
AD  - Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of 
      Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese 
      Academy of Sciences, Donghu South Road 7, Wuhan 430072, China.
FAU - Zeng, Cheng
AU  - Zeng C
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20140602
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - EQ8332842Y (cyanoginosin LR)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/methods
MH  - Glutathione/*analysis/*metabolism
MH  - Limit of Detection
MH  - Liver/*metabolism
MH  - Male
MH  - Marine Toxins
MH  - Microcystins/*analysis/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Solid Phase Extraction/methods
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - LC-ESI-MS/MS
OT  - Microcystin-LR
OT  - Microcystin-LR-Cys
OT  - Microcystin-LR-GSH
OT  - Quantitative determination
OT  - Rat liver
EDAT- 2014/06/16 06:00
MHDA- 2015/02/13 06:00
CRDT- 2014/06/16 06:00
PHST- 2014/02/07 00:00 [received]
PHST- 2014/05/18 00:00 [revised]
PHST- 2014/05/23 00:00 [accepted]
PHST- 2014/06/16 06:00 [entrez]
PHST- 2014/06/16 06:00 [pubmed]
PHST- 2015/02/13 06:00 [medline]
AID - S1570-0232(14)00361-4 [pii]
AID - 10.1016/j.jchromb.2014.05.046 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jul 15;963:54-61. doi: 
      10.1016/j.jchromb.2014.05.046. Epub 2014 Jun 2.