PMID- 24931007
OWN - NLM
STAT- MEDLINE
DCOM- 20150414
LR  - 20240321
IS  - 1530-0277 (Electronic)
IS  - 0145-6008 (Print)
IS  - 0145-6008 (Linking)
VI  - 38
IP  - 7
DP  - 2014 Jul
TI  - Dysmorphogenic effects of first trimester-equivalent ethanol exposure in mice: a 
      magnetic resonance microscopy-based study.
PG  - 2008-14
LID - 10.1111/acer.12464 [doi]
AB  - BACKGROUND: The first trimester of human development and the equivalent 
      developmental period in animal models is a time when teratogenic ethanol (EtOH) 
      exposure induces the major structural birth defects that fall within fetal 
      alcohol spectrum disorder (FASD). Previous FASD research employing an acute high 
      dose maternal intraperitoneal EtOH treatment paradigm has identified sensitive 
      periods for a number of these defects. Extending this work, this investigation 
      utilized high resolution magnetic resonance microscopy (MRM)-based analyses to 
      examine the dysmorphology resulting from maternal dietary EtOH intake occurring 
      during selected first trimester-equivalent time periods. METHODS: Female C57Bl/6J 
      mice were acclimated to a liquid 4.8% EtOH (v/v)-containing diet, then bred while 
      on standard chow. Dams were again provided the EtOH-containing liquid diet for a 
      period that extended either from the beginning of gestational day (GD) 7 to the 
      end of GD 11 or from the beginning of GD 12 to the end of GD 16. On GD 17, a 
      subset of fetuses was selected for MRM-based analyses. Group comparisons were 
      made for litter characteristics and gross dysmorphology, as well as whole and 
      regional brain volumes. RESULTS: EtOH-induced stage of exposure-dependent 
      structural brain abnormalities were observed. The GD 7 to 11 EtOH-exposed group 
      presented with a significant decrease in cerebellar volume and an increase in 
      septal volume, while GD 12 to 16 EtOH treatment resulted in a reduction in right 
      hippocampal volume accompanied by enlarged pituitaries. Additionally, the GD 12 
      to 16 EtOH exposure caused a high incidence of edema/fetal hydrops. CONCLUSIONS: 
      These results illustrate the teratogenic impact of maternal dietary EtOH intake 
      occurring at time periods approximately equivalent to weeks 3 through 6 (GD 7 to 
      11 in mice) and weeks 7 through 12 (GD 12 to 16 in mice) of human gestation, 
      further documenting EtOH's stage of exposure-dependent neuroteratogenic end 
      points and highlighting the vulnerability of selected brain regions during the 
      first trimester. Additionally they suggest that clinical attention should be paid 
      to fetal hydrops as a likely component of FASD.
CI  - Copyright (c) 2014 by the Research Society on Alcoholism.
FAU - Parnell, Scott E
AU  - Parnell SE
AD  - Bowles Center for Alcohol Studies, University of North Carolina, Chapel Hill, 
      North Carolina; Carolina Institute for Developmental Disabilities, University of 
      North Carolina, Chapel Hill, North Carolina; Department of Cell Biology and 
      Physiology, University of North Carolina, Chapel Hill, North Carolina.
FAU - Holloway, Hunter E
AU  - Holloway HE
FAU - Baker, Lorinda K
AU  - Baker LK
FAU - Styner, Martin A
AU  - Styner MA
FAU - Sulik, Kathleen K
AU  - Sulik KK
LA  - eng
GR  - P41 EB015897/EB/NIBIB NIH HHS/United States
GR  - P30-HD03110/HD/NICHD NIH HHS/United States
GR  - U54 HD079124/HD/NICHD NIH HHS/United States
GR  - P30 HD003110/HD/NICHD NIH HHS/United States
GR  - U01-AA021651/AA/NIAAA NIH HHS/United States
GR  - K99 AA018697/AA/NIAAA NIH HHS/United States
GR  - K99/R00-AA018697/AA/NIAAA NIH HHS/United States
GR  - P60-AA011605/AA/NIAAA NIH HHS/United States
GR  - U01-AA017124/AA/NIAAA NIH HHS/United States
GR  - P60 AA011605/AA/NIAAA NIH HHS/United States
GR  - U54 EB005149/EB/NIBIB NIH HHS/United States
GR  - P41-EB015897/EB/NIBIB NIH HHS/United States
GR  - U01 AA017124/AA/NIAAA NIH HHS/United States
GR  - U01 AA021651/AA/NIAAA NIH HHS/United States
GR  - U54-EB005149-01/EB/NIBIB NIH HHS/United States
GR  - R00 AA018697/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140613
PL  - England
TA  - Alcohol Clin Exp Res
JT  - Alcoholism, clinical and experimental research
JID - 7707242
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Abnormalities, Drug-Induced/diagnosis/*pathology
MH  - Animals
MH  - Brain/*abnormalities/*drug effects
MH  - Ethanol/*toxicity
MH  - Female
MH  - Hydrops Fetalis/chemically induced/pathology
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Mice
MH  - Neuroimaging
MH  - Pituitary Gland/abnormalities
MH  - Pregnancy
MH  - *Pregnancy Trimester, First/drug effects
PMC - PMC4107075
MID - NIHMS586695
OTO - NOTNLM
OT  - Brain
OT  - Ethanol
OT  - Fetal Alcohol Spectrum Disorder
OT  - Magnetic Resonance Microscopy
OT  - Mouse
EDAT- 2014/06/17 06:00
MHDA- 2015/04/15 06:00
PMCR- 2015/07/01
CRDT- 2014/06/17 06:00
PHST- 2013/12/16 00:00 [received]
PHST- 2014/04/09 00:00 [accepted]
PHST- 2014/06/17 06:00 [entrez]
PHST- 2014/06/17 06:00 [pubmed]
PHST- 2015/04/15 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - 10.1111/acer.12464 [doi]
PST - ppublish
SO  - Alcohol Clin Exp Res. 2014 Jul;38(7):2008-14. doi: 10.1111/acer.12464. Epub 2014 
      Jun 13.