PMID- 24931255
OWN - NLM
STAT- MEDLINE
DCOM- 20150406
LR  - 20140616
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 127
IP  - 12
DP  - 2014
TI  - Targeting gallbladder carcinoma: bone marrow-derived stem cells as therapeutic 
      delivery vehicles of myxoma virus.
PG  - 2350-6
AB  - BACKGROUND: Gallbladder carcinoma (GBC) has a high mortality rate, requiring 
      synergistic anti-tumor management for effective treatment. The myxoma virus 
      (MYXV) exhibits a modest clinical value through its oncolytic potential and 
      narrow host tropism. METHODS: We performed viral replication assays, cell 
      viability assays, migration assays, and xenograft tumor models to demonstrate 
      that bone marrow-derived stem cells (BMSCs) may enhance efficiency of intravenous 
      MYXV delivery. RESULTS: We examined the permissiveness of various GBC cell lines 
      towards MYXV infection and found two supported single and multiple rounds of MYXV 
      replication, leading to an oncolytic effect. Furthermore, we found that BMSCs 
      exhibited tropism for GBC cells within a Matrigel migration system. BMSCs failed 
      to affect the growth of GBC cells, in terms of tumor volume and survival time. 
      Finally, we demonstrated in vivo that intravenous injection of MYXV-infected 
      BMSCs significantly improves the oncolytic effect of MYXV alone, almost to the 
      same extent as intratumoral injection of MYXV. CONCLUSION: This study indicates 
      that BMSCs are a promising novel vehicle for MYXV to clinically address 
      gallbladder tumors.
FAU - Weng, Mingzhe
AU  - Weng M
AD  - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200092, China.
FAU - Zhang, Mingdi
AU  - Zhang M
AD  - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200092, China.
FAU - Qin, Yiyu
AU  - Qin Y
AD  - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200092, China.
FAU - Gong, Wei
AU  - Gong W
AD  - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200092, China.
FAU - Tang, Zhaohui
AU  - Tang Z
AD  - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200092, China.
FAU - Quan, Zhiwei
AU  - Quan Z
AD  - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200092, China. Email: xinhuawmz@126.com.
FAU - Wu, Kejin
AU  - Wu K
AD  - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200092, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Cell Movement/physiology
MH  - Cell Survival/physiology
MH  - Female
MH  - Gallbladder Neoplasms/*therapy/virology
MH  - Humans
MH  - Immunohistochemistry
MH  - Mice
MH  - Myxoma virus/*pathogenicity
MH  - Stem Cells/*cytology/physiology
MH  - Virus Replication/physiology
MH  - Xenograft Model Antitumor Assays
EDAT- 2014/06/17 06:00
MHDA- 2015/04/07 06:00
CRDT- 2014/06/17 06:00
PHST- 2014/06/17 06:00 [entrez]
PHST- 2014/06/17 06:00 [pubmed]
PHST- 2015/04/07 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2014;127(12):2350-6.