PMID- 24931626
OWN - NLM
STAT- MEDLINE
DCOM- 20150309
LR  - 20140714
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 39
IP  - 2
DP  - 2014 Aug
TI  - In vitro antibacterial analysis of phenoloxidase reaction products from the sea 
      cucumber Apostichopus japonicus.
PG  - 458-63
LID - S1050-4648(14)00201-0 [pii]
LID - 10.1016/j.fsi.2014.06.002 [doi]
AB  - Three phenoloxidases (POs) of Apostichopus japonicus, AjPOs (AjPO1, AjPO2 and 
      AjPO3), were partially purified from the coelomocytes with an electrophoretic 
      method, and then employed for the in vitro antibacterial analysis. Using 
      L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate, AjPO1 and AjPO2-derived 
      compounds inhibited the growth of Vibrio splendidus and Staphylococcus aureus, 
      while AjPO3-derived compounds only inhibited the growth of V. splendidus. When 
      dopamine was used as a substrate, AjPO1 and AjPO3-derived compounds inhibited the 
      growth of V. splendidus and Vibrio harveyi, while AjPO2-derived compounds only 
      inhibited the growth of V. splendidus. Moreover, AjPO1-derived compounds showed 
      stronger inhibition in V. harveyi than AjPO3-derived compounds did. However, all 
      of the three AjPO reaction products showed no inhibitions on the growth of 
      Pseudoalteromonas nigrifaciens, Shewanella baltica, Micrococcus lysodeikticus, 
      Streptococcus dysgalactiae and Nocardiopsis sp. with L-DOPA or dopamine as a 
      substrate. Scanning electron microscope (SEM) observation of V. harveyi treated 
      by AjPOs and dopamine showed that AjPO1-derived compounds resulted in massive 
      bacteriolysis, AjPO2-derived compounds caused no obvious alteration on bacterial 
      morphology, and AjPO3-derived compounds increased the ratio of spheroidal 
      bacteria. All these results suggested that AjPO reaction products derived by 
      L-DOPA and dopamine had different but limited antibacterial spectrum, and the 
      different antibacterial effects observed among three AjPOs resulted from the 
      different reaction products generated by AjPOs with the same substrate.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Jiang, Jingwei
AU  - Jiang J
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
FAU - Zhou, Zunchun
AU  - Zhou Z
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China. 
      Electronic address: zunchunz@hotmail.com.
FAU - Dong, Ying
AU  - Dong Y
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
FAU - Cong, Cong
AU  - Cong C
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
FAU - Guan, Xiaoyan
AU  - Guan X
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
FAU - Wang, Bai
AU  - Wang B
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
FAU - Chen, Zhong
AU  - Chen Z
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
FAU - Jiang, Bei
AU  - Jiang B
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
FAU - Yang, Aifu
AU  - Yang A
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
FAU - Gao, Shan
AU  - Gao S
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
FAU - Sun, Hongjuan
AU  - Sun H
AD  - Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and 
      Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140612
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Anti-Bacterial Agents)
RN  - 46627O600J (Levodopa)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*metabolism/pharmacology
MH  - Bacteriolysis/*drug effects
MH  - In Vitro Techniques
MH  - Levodopa/*metabolism
MH  - Microscopy, Electron, Scanning
MH  - Monophenol Monooxygenase/immunology/isolation & purification/*metabolism
MH  - Sea Cucumbers/*enzymology/*immunology
MH  - Species Specificity
MH  - Staphylococcus aureus/drug effects/growth & development
MH  - Vibrio/drug effects/growth & development/ultrastructure
OTO - NOTNLM
OT  - Antibacterial effects
OT  - Dopamine
OT  - L-3,4-dihydroxyphenylalanine
OT  - Phenoloxidases
OT  - Sea cucumber (Apostichopus japonicus)
EDAT- 2014/06/17 06:00
MHDA- 2015/03/10 06:00
CRDT- 2014/06/17 06:00
PHST- 2014/03/05 00:00 [received]
PHST- 2014/06/01 00:00 [revised]
PHST- 2014/06/05 00:00 [accepted]
PHST- 2014/06/17 06:00 [entrez]
PHST- 2014/06/17 06:00 [pubmed]
PHST- 2015/03/10 06:00 [medline]
AID - S1050-4648(14)00201-0 [pii]
AID - 10.1016/j.fsi.2014.06.002 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2014 Aug;39(2):458-63. doi: 10.1016/j.fsi.2014.06.002. 
      Epub 2014 Jun 12.