PMID- 24935341
OWN - NLM
STAT- MEDLINE
DCOM- 20150226
LR  - 20211203
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 46
IP  - 5
DP  - 2014 Jun
TI  - Effect of inhibiting the signal of mammalian target of rapamycin on memory T 
      cells.
PG  - 1642-8
LID - S0041-1345(14)00149-3 [pii]
LID - 10.1016/j.transproceed.2013.10.063 [doi]
AB  - BACKGROUND: Mammalian target of rapamycin (mTOR) signaling is crucial to the 
      activation and proliferation of T cells. Memory T cells can significantly hinder 
      the induction of transplant tolerance. Resent research demonstrates that mTOR 
      signaling regulates the survival and function of memory T cells. MATERIALS AND 
      METHODS: Naive T cells were adoptively transferred to Rag(-/-) mice to generate 
      similar memory T cells that undergo homeostatic proliferation. These memory T 
      cells were then used to examine the effect of mTOR inhibition on the function of 
      memory T cells. The effect of inhibiting mTOR signaling on the apoptosis of 
      memory T cells was also examined. RESULTS: Quantitative reverse-transcription 
      polymerase chain reaction analysis showed that the expression of mTOR signaling 
      was substantially lower in memory T cells. The levels of interferon-gamma, 
      interleukin (IL)-2, IL-4, and IL-10 decreased after mTOR inhibition; the 
      expression of Bcl-2 increased in memory CD8(+) T cells and decreased in memory 
      CD4(+) T cells; and Bax increased in memory CD4(+) T cells and decreased in 
      memory CD8(+) T cells. Memory CD4(+) T cells were more sensitive to apoptotic 
      cell death in this model after mTOR inhibition. Memory CD8(+) T cells were not 
      affected by mTOR inhibition. CONCLUSIONS: mTOR was crucial to homeostatic 
      proliferation-induced memory T cells. The critical mechanisms of mTOR signaling 
      inhibition are suppressed the functions of memory T cells and promoted the 
      apoptosis of memory CD4(+) T cells.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Chen, S
AU  - Chen S
AD  - Medical School of Southeast University, Department of Urology, Affiliated Zhongda 
      Hospital of Southeast University, Nan Jing, China.
FAU - Liu, D
AU  - Liu D
AD  - Medical School of Southeast University, Department of Urology, Affiliated Zhongda 
      Hospital of Southeast University, Nan Jing, China.
FAU - Wu, J
AU  - Wu J
AD  - Department of Urology, Affiliated Nanjing First Hospital, Nanjing Medical 
      University, Nan Jing, China.
FAU - Xu, B
AU  - Xu B
AD  - Medical School of Southeast University, Department of Urology, Affiliated Zhongda 
      Hospital of Southeast University, Nan Jing, China.
FAU - Lu, K
AU  - Lu K
AD  - Medical School of Southeast University, Department of Urology, Affiliated Zhongda 
      Hospital of Southeast University, Nan Jing, China.
FAU - Zhu, W
AU  - Zhu W
AD  - Medical School of Southeast University, Department of Urology, Affiliated Zhongda 
      Hospital of Southeast University, Nan Jing, China.
FAU - Chen, M
AU  - Chen M
AD  - Medical School of Southeast University, Department of Urology, Affiliated Zhongda 
      Hospital of Southeast University, Nan Jing, China. Electronic address: 
      mingchen6308@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (DNA Primers)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers
MH  - Flow Cytometry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/*antagonists & inhibitors/metabolism
EDAT- 2014/06/18 06:00
MHDA- 2015/02/27 06:00
CRDT- 2014/06/18 06:00
PHST- 2013/08/29 00:00 [received]
PHST- 2013/10/28 00:00 [accepted]
PHST- 2014/06/18 06:00 [entrez]
PHST- 2014/06/18 06:00 [pubmed]
PHST- 2015/02/27 06:00 [medline]
AID - S0041-1345(14)00149-3 [pii]
AID - 10.1016/j.transproceed.2013.10.063 [doi]
PST - ppublish
SO  - Transplant Proc. 2014 Jun;46(5):1642-8. doi: 10.1016/j.transproceed.2013.10.063.