PMID- 2493649
OWN - NLM
STAT- MEDLINE
DCOM- 19890413
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 86
IP  - 5
DP  - 1989 Mar
TI  - Formation and action of 8-hydroxy-11,12-epoxy-5,9,14-icosatrienoic acid in 
      Aplysia: a possible second messenger in neurons.
PG  - 1721-5
AB  - In Aplysia neural tissue, the release and metabolism of arachidonic acid are 
      stimulated by histamine or by activation of the identified L32 nerve cell circuit 
      of the abdominal ganglion. Previously we found that histamine and intracellular 
      stimulation of L32 cells, which are putatively histaminergic neurons, cause the 
      production of 12-hydroxy-5,8,10,14-icosatetraenoic acid (12-HETE), a product of 
      the 12-lipoxygenase pathway formed through 
      12-hydroperoxy-5,8,10,14-icosatetraenoic acid (12-HPETE). 12-HPETE, but not 
      12(S)-HETE, mimics the dual-action response of L14 ink motor neurons to histamine 
      and stimulation of L32. 12-HPETE can also be further metabolized to 
      8-hydroxy-11,12-epoxy-5,9,14-icosatrienoic acid (8-HEpETE) which was identified 
      by HPLC, enzymatic hydrolysis, and GC/MS. Production of 8-HEpETE is specific, as 
      its positional isomer 10-hydroxy-11,12-epoxy-5,8,14-icosatrienoic acid is not 
      formed after physiologic stimulation. 8-HEpETE can elicit the late component 
      (hyperpolarization) of the dual-action response in L14 cells, suggesting that it 
      may be a second messenger in Aplysia.
FAU - Piomelli, D
AU  - Piomelli D
AD  - Howard Hughes Medical Institute, Columbia University, New York, NY 10032.
FAU - Shapiro, E
AU  - Shapiro E
FAU - Zipkin, R
AU  - Zipkin R
FAU - Schwartz, J H
AU  - Schwartz JH
FAU - Feinmark, S J
AU  - Feinmark SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 85589-24-8 (8-hydroxy-11,12-epoxyeicosa-5,9,14-trienoic acid)
RN  - EC 1.13.11.12 (Lipoxygenase)
RN  - FC398RK06S (8,11,14-Eicosatrienoic Acid)
SB  - IM
MH  - 8,11,14-Eicosatrienoic Acid/analogs & derivatives/biosynthesis/*physiology
MH  - Animals
MH  - Aplysia
MH  - Electric Stimulation
MH  - Fatty Acids, Unsaturated/*physiology
MH  - Ganglia/physiology
MH  - Gas Chromatography-Mass Spectrometry
MH  - Isomerism
MH  - Lipoxygenase/metabolism
MH  - Neurons/*physiology
MH  - *Second Messenger Systems
PMC - PMC286773
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
PMCR- 1989/09/01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
PHST- 1989/09/01 00:00 [pmc-release]
AID - 10.1073/pnas.86.5.1721 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1989 Mar;86(5):1721-5. doi: 10.1073/pnas.86.5.1721.