PMID- 24938625
OWN - NLM
STAT- MEDLINE
DCOM- 20150511
LR  - 20211021
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 92
IP  - 11
DP  - 2014 Nov
TI  - Pericyte protection by edaravone after tissue plasminogen activator treatment in 
      rat cerebral ischemia.
PG  - 1509-19
LID - 10.1002/jnr.23420 [doi]
AB  - Pericytes play a pivotal role in contraction, mediating inflammation and 
      regulation of blood flow in the brain. In this study, changes of pericytes in the 
      neurovascular unit (NVU) were examined in relation to the effects of exogenous 
      tissue plasminogen activator (tPA) and a free radical scavenger, edaravone. 
      Immunohistochemistry and Western blot analyses showed that the overlap between 
      platelet-derived growth factor receptor beta-positive pericytes and 
      N-acetylglucosamine oligomers (NAGO)-positive endothelial cells increased 
      significantly at 4 days after 90 min of transient middle cerebral artery 
      occlusion (tMCAO). The number of pericytes and the overlap with NAGO decreased 
      with tPA but recovered with edaravone 4 days after tMCAO with proliferation. 
      Thus, tPA treatment damaged pericytes, resulting in the detachment from 
      astrocytes and a decrease in glial cell line-derived neurotrophic factor 
      secretion. However, treatment with edaravone greatly improved tPA-induced damage 
      to pericytes. The present study demonstrates that exogenous tPA strongly damages 
      pericytes and destroys the integrity of the NVU, but edaravone treatment can 
      greatly ameliorate such damage after acute cerebral ischemia in rats.
CI  - Copyright (c) 2014 The Authors. Journal of Neuroscience Research Published by Wiley 
      Periodicals, Inc.
FAU - Deguchi, Kentaro
AU  - Deguchi K
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Liu, Ning
AU  - Liu N
FAU - Liu, Wentao
AU  - Liu W
FAU - Omote, Yoshio
AU  - Omote Y
FAU - Kono, Syoichiro
AU  - Kono S
FAU - Yunoki, Taijun
AU  - Yunoki T
FAU - Deguchi, Shoko
AU  - Deguchi S
FAU - Yamashita, Toru
AU  - Yamashita T
FAU - Ikeda, Yoshio
AU  - Ikeda Y
FAU - Abe, Koji
AU  - Abe K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140617
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Ki 6896)
RN  - 0 (Quinolines)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor beta)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - S798V6YJRP (Edaravone)
RN  - T3CHA1B51H (Antipyrine)
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine/metabolism
MH  - Animals
MH  - Antipyrine/*analogs & derivatives/pharmacology
MH  - Astrocytes/drug effects/metabolism
MH  - Brain Ischemia/*drug therapy/*pathology
MH  - Cell Proliferation/drug effects
MH  - Disease Models, Animal
MH  - Edaravone
MH  - Free Radical Scavengers/*pharmacology
MH  - Glial Cell Line-Derived Neurotrophic Factor/metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Male
MH  - Pericytes/*drug effects
MH  - Quinolines/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Platelet-Derived Growth Factor beta/metabolism
MH  - Reperfusion
MH  - Time Factors
MH  - Tissue Plasminogen Activator/*adverse effects
PMC - PMC4263311
OTO - NOTNLM
OT  - cerebral ischemia
OT  - edaravone
OT  - neurovascular unit
OT  - pericyte
OT  - tPA
EDAT- 2014/06/19 06:00
MHDA- 2015/05/12 06:00
CRDT- 2014/06/19 06:00
PHST- 2014/02/01 00:00 [received]
PHST- 2014/04/16 00:00 [revised]
PHST- 2014/04/27 00:00 [accepted]
PHST- 2014/06/19 06:00 [entrez]
PHST- 2014/06/19 06:00 [pubmed]
PHST- 2015/05/12 06:00 [medline]
AID - 10.1002/jnr.23420 [doi]
PST - ppublish
SO  - J Neurosci Res. 2014 Nov;92(11):1509-19. doi: 10.1002/jnr.23420. Epub 2014 Jun 
      17.