PMID- 24939016
OWN - NLM
STAT- MEDLINE
DCOM- 20150220
LR  - 20211021
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 15
IP  - 1
DP  - 2014 Jun 17
TI  - Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 
      indicates effects on cell cycle regulation and lipid metabolism.
PG  - 481
LID - 10.1186/1471-2164-15-481 [doi]
LID - 481
AB  - BACKGROUND: Polychlorinated biphenyls (PCBs) are persistent organic pollutants 
      (POPs) with harmful effects in animals and humans. Although PCB 153 is one of the 
      most abundant among PCBs detected in animal tissues, its mechanism of toxicity is 
      not well understood. Only few studies have been conducted to explore genes and 
      pathways affected by PCB 153 by using high throughput transcriptomics approaches. 
      To obtain better insights into toxicity mechanisms, we treated juvenile Atlantic 
      cod (Gadus morhua) with PCB 153 (0.5, 2 and 8 mg/kg body weight) for 2 weeks and 
      performed gene expression analysis in the liver using oligonucleotide arrays. 
      RESULTS: Whole-genome gene expression analysis detected about 160 differentially 
      regulated genes. Functional enrichment, interactome, network and gene set 
      enrichment analysis of the differentially regulated genes suggested that pathways 
      associated with cell cycle, lipid metabolism, immune response, apoptosis and 
      stress response were among the top significantly enriched. Particularly, genes 
      coding for proteins in DNA replication/cell cycle pathways and enzymes of lipid 
      biosynthesis were up-regulated suggesting increased cell proliferation and 
      lipogenesis, respectively. CONCLUSIONS: PCB 153 appears to activate cell 
      proliferation and lipogenic genes in cod liver. Transcriptional up-regulation of 
      marker genes for lipid biosynthesis resembles lipogenic effects previously 
      reported for persistent organic pollutants (POPs) and other environmental 
      chemicals. Our results provide new insights into mechanisms of PCB 153 induced 
      toxicity.
FAU - Yadetie, Fekadu
AU  - Yadetie F
AD  - Department of Molecular Biology, University of Bergen, Bergen, Norway. 
      Fekadu.Yadetie@mbi.uib.no.
FAU - Karlsen, Odd Andre
AU  - Karlsen OA
FAU - Eide, Marta
AU  - Eide M
FAU - Hogstrand, Christer
AU  - Hogstrand C
FAU - Goksoyr, Anders
AU  - Goksoyr A
LA  - eng
SI  - GEO/GSE43733
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140617
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - ZRU0C9E32O (2,4,5,2',4',5'-hexachlorobiphenyl)
SB  - IM
MH  - Animals
MH  - Cell Cycle/*drug effects
MH  - Cluster Analysis
MH  - Computational Biology
MH  - Gadus morhua/*genetics/*metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Gene Regulatory Networks
MH  - Lipid Metabolism/*drug effects
MH  - Liver/*drug effects/*metabolism
MH  - Molecular Sequence Annotation
MH  - Polychlorinated Biphenyls/*pharmacology
MH  - Signal Transduction
MH  - *Transcriptome
PMC - PMC4078240
EDAT- 2014/06/19 06:00
MHDA- 2015/02/24 06:00
PMCR- 2014/06/17
CRDT- 2014/06/19 06:00
PHST- 2014/04/01 00:00 [received]
PHST- 2014/06/11 00:00 [accepted]
PHST- 2014/06/19 06:00 [entrez]
PHST- 2014/06/19 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2014/06/17 00:00 [pmc-release]
AID - 1471-2164-15-481 [pii]
AID - 6174 [pii]
AID - 10.1186/1471-2164-15-481 [doi]
PST - epublish
SO  - BMC Genomics. 2014 Jun 17;15(1):481. doi: 10.1186/1471-2164-15-481.