PMID- 24939571
OWN - NLM
STAT- MEDLINE
DCOM- 20150420
LR  - 20150204
IS  - 1468-3288 (Electronic)
IS  - 0017-5749 (Linking)
VI  - 64
IP  - 3
DP  - 2015 Mar
TI  - The HLA-DQ2 genotype selects for early intestinal microbiota composition in 
      infants at high risk of developing coeliac disease.
PG  - 406-17
LID - 10.1136/gutjnl-2014-306931 [doi]
AB  - OBJECTIVE: Intestinal dysbiosis has been associated with coeliac disease (CD), 
      but whether the alterations are cause or consequence of the disease is unknown. 
      This study investigated whether the human leukocyte antigen (HLA)-DQ2 genotype is 
      an independent factor influencing the early gut microbiota composition of healthy 
      infants at family risk of CD. DESIGN: As part of a larger prospective study, a 
      subset (n=22) of exclusively breastfed and vaginally delivered infants with 
      either high genetic risk (HLA-DQ2 carriers) or low genetic risk (non-HLA-DQ2/8 
      carriers) of developing CD were selected from a cohort of healthy infants with at 
      least one first-degree relative with CD. Infant faecal microbiota was analysed by 
      16S rRNA gene pyrosequencing and real time quantitative PCR. RESULTS: Infants 
      with a high genetic risk had significantly higher proportions of Firmicutes and 
      Proteobacteria and lower proportions of Actinobacteria compared with low-risk 
      infants. At genus level, high-risk infants had significantly less Bifidobacterium 
      and unclassified Bifidobacteriaceae proportions and more Corynebacterium, 
      Gemella, Clostridium sensu stricto, unclassified Clostridiaceae, unclassified 
      Enterobacteriaceae and Raoultella proportions. Quantitative real time PCR also 
      revealed lower numbers of Bifidobacterium species in infants with high genetic 
      risk than in those with low genetic risk. In high-risk infants negative 
      correlations were identified between Bifidobacterium species and several genera 
      of Proteobacteria (Escherichia/Shigella) and Firmicutes (Clostridium). 
      CONCLUSIONS: The genotype of infants at family risk of developing CD, carrying 
      the HLA-DQ2 haplotypes, influences the early gut microbiota composition. This 
      finding suggests that a specific disease-biased host genotype may also select for 
      the first gut colonisers and could contribute to determining disease risk.
CI  - Published by the BMJ Publishing Group Limited. For permission to use (where not 
      already granted under a licence) please go to 
      http://group.bmj.com/group/rights-licensing/permissions.
FAU - Olivares, M
AU  - Olivares M
AD  - Instituto de Agroquimica y Tecnologia de Alimentos, Consejo Superior de 
      Investigaciones Cientificas (IATA-CSIC), Valencia, Spain.
FAU - Neef, A
AU  - Neef A
AD  - Instituto de Agroquimica y Tecnologia de Alimentos, Consejo Superior de 
      Investigaciones Cientificas (IATA-CSIC), Valencia, Spain.
FAU - Castillejo, G
AU  - Castillejo G
AD  - Hospital Universitario Sant Joan de Reus, Tarragona, Spain.
FAU - Palma, G De
AU  - Palma GD
AD  - Instituto de Agroquimica y Tecnologia de Alimentos, Consejo Superior de 
      Investigaciones Cientificas (IATA-CSIC), Valencia, Spain.
FAU - Varea, V
AU  - Varea V
AD  - Gastroenterologia, Nutricion y Hepatologia Pediatrica, Hospital Universitario 
      Sant Joan de Deu and Unidad de Gastroenterologia Pediatrica del Institut Dexeus, 
      Barcelona, Spain.
FAU - Capilla, A
AU  - Capilla A
AD  - Centro de Investigacion Principe Felipe (CIPF) and IBV-CSIC Associated Unit, 
      CIBER de Enfermedades Raras (CIBERER), Valencia, Spain.
FAU - Palau, F
AU  - Palau F
AD  - Centro de Investigacion Principe Felipe (CIPF) and IBV-CSIC Associated Unit, 
      CIBER de Enfermedades Raras (CIBERER), Valencia, Spain.
FAU - Nova, E
AU  - Nova E
AD  - Department Metabolismo y Nutricion, ICTAN-CSIC, Madrid, Spain.
FAU - Marcos, A
AU  - Marcos A
AD  - Department Metabolismo y Nutricion, ICTAN-CSIC, Madrid, Spain.
FAU - Polanco, I
AU  - Polanco I
AD  - Servicio de Gastroenterologia y Nutricion Pediatrica, Hospital Universitario La 
      Paz, Madrid, Spain.
FAU - Ribes-Koninckx, C
AU  - Ribes-Koninckx C
AD  - Unidad de Gastroenterologia Pediatrica, Hospital Universitario La Fe, Valencia, 
      Spain.
FAU - Ortigosa, L
AU  - Ortigosa L
AD  - Unidad de Gastroenterologia, Hepatologia y Nutricion Pediatrica, Hospital 
      Universitario Nuestra Senora de Candelaria, Santa Cruz de Tenerife, Canarias, 
      Spain.
FAU - Izquierdo, L
AU  - Izquierdo L
AD  - Instituto de Agroquimica y Tecnologia de Alimentos, Consejo Superior de 
      Investigaciones Cientificas (IATA-CSIC), Valencia, Spain.
FAU - Sanz, Y
AU  - Sanz Y
AD  - Instituto de Agroquimica y Tecnologia de Alimentos, Consejo Superior de 
      Investigaciones Cientificas (IATA-CSIC), Valencia, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140617
PL  - England
TA  - Gut
JT  - Gut
JID - 2985108R
RN  - 0 (Genetic Markers)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ2 antigen)
SB  - IM
MH  - Celiac Disease/*genetics/microbiology
MH  - Clostridium/genetics
MH  - Feces/microbiology
MH  - Female
MH  - Genetic Markers/genetics
MH  - Genotype
MH  - HLA-DQ Antigens/*genetics
MH  - Haplotypes/genetics
MH  - Humans
MH  - Infant
MH  - Intestines/*microbiology
MH  - Male
MH  - Microbiota/*genetics
MH  - Prospective Studies
MH  - Real-Time Polymerase Chain Reaction
MH  - Risk Factors
OTO - NOTNLM
OT  - Bifidobacteria
OT  - Celiac Disease
OT  - Intestinal Bacteria
EDAT- 2014/06/19 06:00
MHDA- 2015/04/22 06:00
CRDT- 2014/06/19 06:00
PHST- 2014/06/19 06:00 [entrez]
PHST- 2014/06/19 06:00 [pubmed]
PHST- 2015/04/22 06:00 [medline]
AID - gutjnl-2014-306931 [pii]
AID - 10.1136/gutjnl-2014-306931 [doi]
PST - ppublish
SO  - Gut. 2015 Mar;64(3):406-17. doi: 10.1136/gutjnl-2014-306931. Epub 2014 Jun 17.