PMID- 24939607
OWN - NLM
STAT- MEDLINE
DCOM- 20150407
LR  - 20211021
IS  - 1868-601X (Electronic)
IS  - 1868-4483 (Linking)
VI  - 5
IP  - 5
DP  - 2014 Oct
TI  - Gellan sulfate core platinum coil with tenascin-C promotes intra-aneurysmal 
      organization in rats.
PG  - 595-603
LID - 10.1007/s12975-014-0352-z [doi]
AB  - The aims of this study were to develop a new coil, gellan sulfate core platinum 
      coil (GSCC), that delivers tenascin-C (TNC) to an aneurysm (GSCC-TNC) and to 
      evaluate the effects on intra-aneurysmal organization. We performed in vitro 
      adsorption tests of TNC to gellan sulfate (GS). GSCC-TNC was produced by 
      immersing GSCC in TNC solution under the following conditions (TNC concentration 
      10, 50, or 100 mug/mL; TNC immersion time 15, 30, or 60 min) by monitoring 
      intra-aneurysmal organization in a rat blind-ended aneurysm model. In addition, 
      20 rats randomly underwent implantation of a platinum coil or the GSCC-TNC 
      produced under optimum conditions into an aneurysm, whose organization effects 
      were compared in a blind fashion at 2 weeks post-surgery. GS demonstrated a high 
      affinity to TNC in a dose-dependent fashion (affinity constant = 1.79 x 10(10) 
      (M(-1))). GSCC immersed in 10 mug/mL of TNC solution for 30 and 60 min induced 
      similar and better organization of aneurysmal cavity compared with that for 
      15 min (the ratio of the organized areas in an aneurysmal cavity-15 min, 
      27.2 +/- 11.8 %; 30 min, 75.6 +/- 11.9 %; 60 min, 82.6 +/- 19.7 %, respectively) with 
      the preservation of the aneurysmal wall structure, while higher TNC 
      concentrations caused the destruction of the aneurysmal wall. GSCC-TNC produced 
      under 10 mug/mL of TNC solution for 30 min showed a significantly better 
      organization of aneurysms compared with bare platinum coils in rats. A newly 
      developed coil, GSCC-TNC, may be effective for improving intra-aneurysmal 
      organization after coil embolization.
FAU - Hamada, Kazuhide
AU  - Hamada K
AD  - Department of Neurosurgery, Mie University Graduate School of Medicine, 2-174 
      Edobashi, Tsu, Mie, 514-8507, Japan.
FAU - Miura, Yoichi
AU  - Miura Y
FAU - Toma, Naoki
AU  - Toma N
FAU - Miyamoto, Keiichi
AU  - Miyamoto K
FAU - Imanaka-Yoshida, Kyoko
AU  - Imanaka-Yoshida K
FAU - Matsushima, Satoshi
AU  - Matsushima S
FAU - Yoshida, Toshimichi
AU  - Yoshida T
FAU - Taki, Waro
AU  - Taki W
FAU - Suzuki, Hidenori
AU  - Suzuki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140619
PL  - United States
TA  - Transl Stroke Res
JT  - Translational stroke research
JID - 101517297
RN  - 0 (Polysaccharides)
RN  - 0 (Sulfuric Acid Esters)
RN  - 0 (Tenascin)
RN  - 0 (gellan sulfate)
RN  - 49DFR088MY (Platinum)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Embolization, Therapeutic/*instrumentation
MH  - Intracranial Aneurysm/pathology/*therapy
MH  - Male
MH  - Platinum
MH  - Polysaccharides
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sulfuric Acid Esters
MH  - Tenascin/*administration & dosage/pharmacokinetics
EDAT- 2014/06/19 06:00
MHDA- 2015/04/08 06:00
CRDT- 2014/06/19 06:00
PHST- 2014/03/26 00:00 [received]
PHST- 2014/06/04 00:00 [accepted]
PHST- 2014/05/11 00:00 [revised]
PHST- 2014/06/19 06:00 [entrez]
PHST- 2014/06/19 06:00 [pubmed]
PHST- 2015/04/08 06:00 [medline]
AID - 10.1007/s12975-014-0352-z [doi]
PST - ppublish
SO  - Transl Stroke Res. 2014 Oct;5(5):595-603. doi: 10.1007/s12975-014-0352-z. Epub 
      2014 Jun 19.