PMID- 24939730 OWN - NLM STAT- MEDLINE DCOM- 20150330 LR - 20211108 IS - 1460-2407 (Electronic) IS - 1360-9947 (Linking) VI - 20 IP - 9 DP - 2014 Sep TI - Menstrual blood-derived stromal stem cells from women with and without endometriosis reveal different phenotypic and functional characteristics. PG - 905-18 LID - 10.1093/molehr/gau044 [doi] AB - Retrograde flow of menstrual blood cells during menstruation is considered as the dominant theory for the development of endometriosis. Moreover, current evidence suggests that endometrial-derived stem cells are key players in the pathogenesis of endometriosis. In particular, endometrial stromal stem cells have been suggested to be involved in the pathogenesis of this disease. Here, we aimed to use menstrual blood, as a novel source of endometrial stem cells, to investigate whether stromal stem cells from endometriosis (E-MenSCs) and non-endometriosis (NE-MenSCs) women differed regarding their morphology, CD marker expression pattern, proliferation, invasion and adhesion capacities and their ability to express certain immunomodulatory molecules. E-MenSCs were morphologically different from NE-MenSCs and showed higher expression of CD9, CD10 and CD29. Furthermore, E-MenSCs had higher proliferation and invasion potentials compared with NE-MenSCs. The amount of indoleamine 2,3-dioxygenase-1 (IDO1) and cyclooxygenase-2 (COX-2) in E-MenSCs co-cultured with allogenic peripheral blood mononuclear cells (PBMCs) was shown to be higher both at the gene and protein levels, and higher IDO1 activity was detected in the endometriosis group. However, NE-MenSCs revealed increased concentrations of forkhead transcription factor-3 (FOXP3) when compared with E-MenSCs. Nonetheless, interferon (IFN)-gamma, Interleukin (IL)-10 and monocyte chemoattractant protein-1 (MCP-1) levels were higher in the supernatant of E-MenSCs-PBMC co-cultures. Here, we showed that there are inherent differences between E-MenSCs and NE-MenSCs. These findings propose the key role MenSCs could play in the pathogenesis of endometriosis and further support the retrograde and stem cell theories of endometriosis. Hence, considering its renewable and easily available nature, menstrual blood could be viewed as a reliable and inexpensive material for studies addressing the cellular and molecular aspects of endometriosis. CI - (c) The Author 2014. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oup.com. FAU - Nikoo, Shohreh AU - Nikoo S AD - Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, PO Box 19615-1177, Tehran, Iran. FAU - Ebtekar, Massoumeh AU - Ebtekar M AD - Department of Immunology, Faculty of Medicine, Tarbiat Modares University, PO Box 14117-13116, Tehran, Iran zarnani@aviccena.ac.ir ebtekarm@modares.ac.ir. FAU - Jeddi-Tehrani, Mahmood AU - Jeddi-Tehrani M AD - Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. FAU - Shervin, Adel AU - Shervin A AD - Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, PO Box 19615-1177, Tehran, Iran. FAU - Bozorgmehr, Mahmood AU - Bozorgmehr M AD - Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. FAU - Vafaei, Sedigheh AU - Vafaei S AD - Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. FAU - Kazemnejad, Somayeh AU - Kazemnejad S AD - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. FAU - Zarnani, Amir-Hassan AU - Zarnani AH AD - Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, PO Box 19615-1177, Tehran, Iran Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran zarnani@aviccena.ac.ir ebtekarm@modares.ac.ir. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140616 PL - England TA - Mol Hum Reprod JT - Molecular human reproduction JID - 9513710 RN - 0 (Biomarkers) RN - 0 (Cytokines) RN - 0 (IDO1 protein, human) RN - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase) RN - EC 1.14.99.1 (Cyclooxygenase 2) RN - EC 1.14.99.1 (PTGS2 protein, human) SB - IM MH - Adult MH - Adult Stem Cells/enzymology/metabolism/*pathology MH - Biomarkers/metabolism MH - Cell Communication MH - Cell Line, Tumor MH - Cell Movement MH - Cell Proliferation MH - Cells, Cultured MH - Coculture Techniques MH - Cyclooxygenase 2/genetics/metabolism MH - Cytokines/metabolism MH - Endometriosis/immunology/metabolism/*pathology/physiopathology MH - Endometrium/cytology/metabolism/*pathology MH - Female MH - Humans MH - Indoleamine-Pyrrole 2,3,-Dioxygenase/genetics/metabolism MH - Iran MH - Leukocytes, Mononuclear/immunology/metabolism/pathology MH - *Menstruation MH - Middle Aged MH - Severity of Illness Index MH - Stromal Cells/enzymology/metabolism/*pathology MH - Up-Regulation MH - Young Adult OTO - NOTNLM OT - IDO1 OT - endometriosis OT - menstrual blood OT - stromal stem cells OT - surface marker expression EDAT- 2014/06/19 06:00 MHDA- 2015/03/31 06:00 CRDT- 2014/06/19 06:00 PHST- 2014/06/19 06:00 [entrez] PHST- 2014/06/19 06:00 [pubmed] PHST- 2015/03/31 06:00 [medline] AID - gau044 [pii] AID - 10.1093/molehr/gau044 [doi] PST - ppublish SO - Mol Hum Reprod. 2014 Sep;20(9):905-18. doi: 10.1093/molehr/gau044. Epub 2014 Jun 16.