PMID- 24939876
OWN - NLM
STAT- MEDLINE
DCOM- 20140902
LR  - 20220311
IS  - 1549-5477 (Electronic)
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 28
IP  - 13
DP  - 2014 Jul 1
TI  - Evolutionally dynamic L1 regulation in embryonic stem cells.
PG  - 1397-409
LID - 10.1101/gad.241661.114 [doi]
AB  - Mobile elements are important evolutionary forces that challenge genomic 
      integrity. Long interspersed element-1 (L1, also known as LINE-1) is the only 
      autonomous transposon still active in the human genome. It displays an unusual 
      pattern of evolution, with, at any given time, a single active L1 lineage 
      amplifying to thousands of copies before getting replaced by a new lineage, 
      likely under pressure of host restriction factors, which act notably by silencing 
      L1 expression during early embryogenesis. Here, we demonstrate that in human 
      embryonic stem (hES) cells, KAP1 (KRAB [Kruppel-associated box domain]-associated 
      protein 1), the master cofactor of KRAB-containing zinc finger proteins 
      (KRAB-ZFPs) previously implicated in the restriction of endogenous retroviruses, 
      represses a discrete subset of L1 lineages predicted to have entered the 
      ancestral genome between 26.8 million and 7.6 million years ago. In mice, we 
      documented a similar chronologically conditioned pattern, albeit with a much 
      contracted time scale. We could further identify an L1-binding KRAB-ZFP, 
      suggesting that this rapidly evolving protein family is more globally responsible 
      for L1 recognition. KAP1 knockdown in hES cells induced the expression of 
      KAP1-bound L1 elements, but their younger, human-specific counterparts (L1Hs) 
      were unaffected. Instead, they were stimulated by depleting DNA 
      methyltransferases, consistent with recent evidence demonstrating that the 
      PIWI-piRNA (PIWI-interacting RNA) pathway regulates L1Hs in hES cells. 
      Altogether, these data indicate that the early embryonic control of L1 is an 
      evolutionarily dynamic process and support a model in which newly emerged 
      lineages are first suppressed by DNA methylation-inducing small RNA-based 
      mechanisms before KAP1-recruiting protein repressors are selected.
CI  - (c) 2014 Castro-Diaz et al.; Published by Cold Spring Harbor Laboratory Press.
FAU - Castro-Diaz, Nathaly
AU  - Castro-Diaz N
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
FAU - Ecco, Gabriela
AU  - Ecco G
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
FAU - Coluccio, Andrea
AU  - Coluccio A
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
FAU - Kapopoulou, Adamandia
AU  - Kapopoulou A
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
FAU - Yazdanpanah, Benyamin
AU  - Yazdanpanah B
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
FAU - Friedli, Marc
AU  - Friedli M
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
FAU - Duc, Julien
AU  - Duc J
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
FAU - Jang, Suk Min
AU  - Jang SM
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
FAU - Turelli, Priscilla
AU  - Turelli P
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
FAU - Trono, Didier
AU  - Trono D
AD  - School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 
      Lausanne, Switzerland.
LA  - eng
SI  - GEO/GSE57989
SI  - GEO/GSE58323
GR  - 268721/ERC_/European Research Council/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140617
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Repressor Proteins)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.3.2.27 (TRIM28 protein, human)
RN  - EC 2.3.2.27 (Tripartite Motif-Containing Protein 28)
SB  - IM
CIN - Genes Dev. 2014 Jul 1;28(13):1381-3. PMID: 24990961
MH  - Animals
MH  - DNA (Cytosine-5-)-Methyltransferases/metabolism
MH  - DNA Methylation
MH  - Embryonic Stem Cells
MH  - Evolution, Molecular
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Long Interspersed Nucleotide Elements/*genetics
MH  - Mice
MH  - Repressor Proteins/genetics
MH  - Tripartite Motif-Containing Protein 28
PMC - PMC4083085
OTO - NOTNLM
OT  - DNA methylation
OT  - KAP1
OT  - KRAB-ZFPs
OT  - LINE1
OT  - embryonic stem cells
OT  - evolution
EDAT- 2014/06/19 06:00
MHDA- 2014/09/03 06:00
PMCR- 2015/01/01
CRDT- 2014/06/19 06:00
PHST- 2014/06/19 06:00 [entrez]
PHST- 2014/06/19 06:00 [pubmed]
PHST- 2014/09/03 06:00 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - gad.241661.114 [pii]
AID - 10.1101/gad.241661.114 [doi]
PST - ppublish
SO  - Genes Dev. 2014 Jul 1;28(13):1397-409. doi: 10.1101/gad.241661.114. Epub 2014 Jun 
      17.