PMID- 24940559
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140618
LR  - 20211021
IS  - 2164-7844 (Print)
IS  - 2164-7860 (Electronic)
IS  - 2164-7844 (Linking)
VI  - 3
IP  - 3
DP  - 2014 Jun 1
TI  - An in vitro culture system that supports robust expansion and maintenance of in 
      vivo engraftment capabilities for myogenic progenitor cells from adult mice.
PG  - 79-87
LID - 10.1089/biores.2014.0007 [doi]
AB  - Muscle cell therapy and tissue engineering require large numbers of functional 
      muscle precursor/progenitor cells (MPCs), making the in vitro expansion of MPCs a 
      critical step for these applications. The cells must maintain their myogenic 
      properties upon robust expansion, especially for cellular therapy applications, 
      in order to achieve efficacious treatment. A major obstacle associated with MPCs 
      expansion is the loss of "stemness," or regenerative capacity, of freshly 
      isolated cells, presumably due to the absence of the native cellular niches. In 
      the current study, we developed an in vitro system that allowed for long-term 
      culture and massive expansion of murine MPCs (mMPCs) with the preservation of 
      myogenic regeneration capabilities. Long term in vitro expanded mMPC expressed 
      the myogenic stem cell markers Pax3 and Pax7 and formed spontaneously contracting 
      myotubes. Furthermore, expanded mMPC injected into the tibialis anterior muscle 
      of nude mice engrafted and formed myofibers. Collectively, the method developed 
      in this study can be potentially adapted for the expansion of human MPCs to high 
      enough numbers for treatment of muscle injuries in human patients.
FAU - Wang, Zhan
AU  - Wang Z
AD  - Wake Forest Institute for Regenerative Medicine , Winston-Salem, North Carolina.
FAU - Cheung, Daniel
AU  - Cheung D
AD  - Oregon State University , School of Chemical, Biological, and Environmental 
      Engineering, Corvallis, Oregon.
FAU - Zhou, Yu
AU  - Zhou Y
AD  - Wake Forest Institute for Regenerative Medicine , Winston-Salem, North Carolina.
FAU - Han, Changjie
AU  - Han C
AD  - Wake Forest Institute for Regenerative Medicine , Winston-Salem, North Carolina.
FAU - Fennelly, Colin
AU  - Fennelly C
AD  - Wake Forest Institute for Regenerative Medicine , Winston-Salem, North Carolina.
FAU - Criswell, Tracy
AU  - Criswell T
AD  - Wake Forest Institute for Regenerative Medicine , Winston-Salem, North Carolina. 
      ; Wake Forest School of Medicine , Winston-Salem, North Carolina.
FAU - Soker, Shay
AU  - Soker S
AD  - Wake Forest Institute for Regenerative Medicine , Winston-Salem, North Carolina. 
      ; Wake Forest School of Medicine , Winston-Salem, North Carolina.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biores Open Access
JT  - BioResearch open access
JID - 101579333
PMC - PMC4048971
OTO - NOTNLM
OT  - cell culture
OT  - cell transplantation
OT  - cellular therapy
OT  - muscle stem cell
EDAT- 2014/06/19 06:00
MHDA- 2014/06/19 06:01
PMCR- 2014/06/01
CRDT- 2014/06/19 06:00
PHST- 2014/06/19 06:00 [entrez]
PHST- 2014/06/19 06:00 [pubmed]
PHST- 2014/06/19 06:01 [medline]
PHST- 2014/06/01 00:00 [pmc-release]
AID - 10.1089/biores.2014.0007 [pii]
AID - 10.1089/biores.2014.0007 [doi]
PST - ppublish
SO  - Biores Open Access. 2014 Jun 1;3(3):79-87. doi: 10.1089/biores.2014.0007.