PMID- 24940817
OWN - NLM
STAT- MEDLINE
DCOM- 20150306
LR  - 20191210
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Linking)
VI  - 17
IP  - 7
DP  - 2014 Jul
TI  - Anti-tumor effects of flavonoids from the ethnic medicine Docynia delavayi 
      (Franch.) Schneid. and its possible mechanism.
PG  - 787-94
LID - 10.1089/jmf.2013.2886 [doi]
AB  - This study investigated the active components and the anti-tumor efficacy and 
      mechanisms of the flavonoids from Docynia delavayi (Franch.) Schneid. (DDS). MTT 
      assay was used to examine the growth inhibitory effects of the four flavonoids, 
      including chrysin, quercetin, naringenin, and avicularin that were isolated from 
      the rhizome of DDS, on human hematomas cell (HepG2), esophageal carcinoma cell 
      (EC109), human cervical adenocarcinoma cell (Hela), human colon adenocarcinoma 
      cell (SW480), and African green monkey kidney cell (Vero cells). The anti-tumor 
      mechanism of chrysin on HepG2 was further investigated by the methods of 
      fluorescence staining, flow cytometry, and immunoblotting. The results showed 
      that the inhibitory activity of chrysin was much stronger than the other three 
      flavonoids on HepG2, EC109, Hela, and SW480 cells for 48 h treatment in vitro. 
      Moreover, no inhibiting effect of chrysin on the proliferation of normal cells 
      (Vero cells) was observed. Further study revealed that chrysin caused HepG2 cell 
      shrinkage, membrane blebbing, and apoptotic body formation, all of which were 
      typical characteristics of apoptosis programmed cell death. Flow cytometric 
      analysis demonstrated that chrysin increased the sub G0/G1 population, which 
      indicated the increased cell apoptosis, thus preventing cells from entering the S 
      phase as the population in G2/M or S phase declined; whereas in G0/G1 phase, it 
      increased. In addition, immunoblot results showed that chrysin significantly 
      increased the expression levels of caspase-3 and Bax proteins, and it decreased 
      the expression level of B-cell lymphoma/leukemia-2 (Bcl-2) protein. These 
      findings indicate that chrysin is the major flavonoid present in DDS, and it 
      induces HepG2 cell death via apoptosis, probably through the participation of 
      caspase-3, Bax, and Bcl-2 proteins.
FAU - Deng, Xukun
AU  - Deng X
AD  - 1 School of Pharmacy, South-Central University for Nationalities , Wuhan, 
      People's Republic of China .
FAU - Zhao, Xiangpei
AU  - Zhao X
FAU - Lan, Zhou
AU  - Lan Z
FAU - Jiang, Jie
AU  - Jiang J
FAU - Yin, Wu
AU  - Yin W
FAU - Chen, Lvyi
AU  - Chen L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140618
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Antineoplastic Agents)
RN  - 0 (BAX protein, human)
RN  - 0 (Flavonoids)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 3CN01F5ZJ5 (chrysin)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Caspase 3/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Chlorocebus aethiops
MH  - Down-Regulation
MH  - Flavonoids/*pharmacology
MH  - HeLa Cells
MH  - Hep G2 Cells
MH  - Humans
MH  - Rosaceae/*chemistry
MH  - Up-Regulation
MH  - Vero Cells
MH  - bcl-2-Associated X Protein/genetics/metabolism
OTO - NOTNLM
OT  - Docynia delavayi (Franch.) Schneid.
OT  - HepG2
OT  - anti-tumor
OT  - apoptosis
OT  - chrysin
OT  - flavonoids
EDAT- 2014/06/19 06:00
MHDA- 2015/03/07 06:00
CRDT- 2014/06/19 06:00
PHST- 2014/06/19 06:00 [entrez]
PHST- 2014/06/19 06:00 [pubmed]
PHST- 2015/03/07 06:00 [medline]
AID - 10.1089/jmf.2013.2886 [doi]
PST - ppublish
SO  - J Med Food. 2014 Jul;17(7):787-94. doi: 10.1089/jmf.2013.2886. Epub 2014 Jun 18.